ToyoScreen® Ether-650M Process Development Column 821373

Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.
Clean in place with 0.5 M NaOH or 0.1 M HCl.
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
ToyoScreen is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation

material	aluminum seal, polypropylene and polyethylene frit
product line	ToyoScreen®
analyte chemical class(es)	proteins
packaging	pkg of 6 x 5 mL
Торговая марка	Tosoh
parameter	50% organic solvent, 1-5 mL/min flow rate, 20 mL/min max. flow rate, 3 bar max. pressure
application(s)	LPLC: suitable
L x I.D.	3 cm x 14.6 mm
matrix	hydroxylated methacrylic polymer
matrix active group	ether phase
particle size	65 µm
operating pH	1 - 13
capacity	20 g/L±10 adsorption capacity (lysozyme)
separation technique	hydrophobic interaction (HIC)