Transfection Sigma-Aldrich
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-Tri-Calcium phosphate
Empirical Formula (Hill Notation): Ca3O8P2 Linear Formula: Ca3(PO4)2 CAS Number: 7758-87-4 Molecular Weight: 310.18 EC Number: 231-840-8 MDL number: MFCD00015984 PubChem Substance ID: 329757609 NACRES: NA.52
Кат. номер 50553-10G-F 50553-VAR-F 50553-1G-F General descriptionα-tri-Calcium phosphate (α-TCP) can be made by heating the low-temperature polymorph β-TCP. It can also be made by thermal crystallization of amorphous precursors with the appropriate composition above the transformation temperature. α-TCP is biocompatible as β-TCP. It is highly soluble and hydrolyses quickly to calcium-deficient hydroxyapatite. This makes α-TCP a beneficial constituent for formulating self-setting osteotransductive bone cements and biodegradable bioceramics and composites for bone repairing.
α-tri-Calcium is a tasteless powder containing excess of calcium oxide. It is commonly used to introduce DNA and transfect eukaryotic cells for molecular biology experiments.ApplicationFor analysis of bone cement; as standard.
-tri-Calcium phosphate has been used to transfect 293T and 231 cells with the PiggyBac LSS-mOrange- aspartate-glutamate-valine-aspartate (DEVD)-mKate2 transposon vector and in reporter verification studies.
Suitable for- transient and stable transfection of wide range of cell types
- drug delivery systems
ПараметрыQuality Level 100 grade for molecular biology form powder application(s) transfection: suitable Featured Industry Cleaning Products
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Personal Carestorage temp. room temp SMILES string [Ca++].[Ca++].[Ca++].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O InChI 1S/3Ca.2H3O4P/c;;;2*1-5(2,3)4/h;;;2*(H3,1,2,3,4)/q3*+2;;/p-6 InChI key QORWJWZARLRLPR-UHFFFAOYSA-H
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Calcium Phosphate Transfection Kit
EC Number: 233-140-8 NACRES: NA.85
Кат. номер CAPHOS-1KT CAPHOS-1KT-PW General descriptionCalcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.ApplicationCalcium Phosphate Transfection Kit has been used:- to enable transfection
- to transfect Hek293T cells
- to transfect H29D cells
Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47DFeatures and Benefits- Suitable for transient and stable transfection
- Reproducible for a wide range of cell types
- Widely referenced
- Inexpensive
ComponentsThe Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)PrincipleThe procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.Other NotesThe protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.ПараметрыQuality Level 200 grade for molecular biology form solution usage kit sufficient for 160 transfections (6 cm dishes), kit sufficient for 400 transfections (3.5 cm dishes), kit sufficient for 80 transfections (10 cm dishes) application(s) transfection: suitable shipped in dry ice storage temp. −20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H319 pcodes P305 + P351 + P338 RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
DAPI
Empirical Formula (Hill Notation): C16H15N5 · 2HCl CAS Number: 28718-90-3 Molecular Weight: 350.25 Beilstein/REAXYS Number: 4894417 EC Number: 249-186-7 MDL number: MFCD00012681 PubChem Substance ID: 24894305 NACRES: NA.52
Кат. номер D9542-50MG D9542-1MG D9542-10MG D9542-5MG D9542-50MG-PW D9542-1MG-PW D9542-10MG-PW D9542-5MG-PW General descriptionDAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.ApplicationSuitable for- DNA staining in agarose gels
- analysis of changes in DNA during apoptosis
- detection of mycoplasma
- photofootprinting of DNA
- immunofluorescent staining of cells
DAPI has been used:-- in rapid monitoring of microbial contamination
- in chromosomal banding technique
- in detection of apoptotic cells
- in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
- to stain mature pollen grains(0.5 mg/ml)
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.Packaging1, 5, 10, 50 mg in glass bottleBiochem/physiol ActionsCell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.CautionProtect from light.ПараметрыQuality Level 300 grade for molecular biology assay 98% (HPLC and TLC) form powder application(s) transfection: suitable solubility H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.), PBS: insoluble (extinction coefficient) 30 at 263 nm in H2O at 1 mM fluorescence ex 340 nm; em 488 nm (nur DAPI), ex 364 nm; em 454 nm (DAPI-DNA-Komplex) suitability suitable for fluorescence storage temp. 2-8°C SMILES string Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N InChI 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H InChI key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H317 - H335 pcodes P261 - P264 - P271 - P272 - P280 - P302 + P352 Target Organs Respiratory system Personal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
DAPI ready made solution
Empirical Formula (Hill Notation): C16H15N5 · 2HCl CAS Number: 28718-90-3 Molecular Weight: 350.25 Beilstein/REAXYS Number: 4894417 MDL number: MFCD00012681
Кат. номер MBD0015-10ML MBD0015-5ML MBD0015-50ML MBD0015-1ML General descriptionDAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.ApplicationDAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1µg/mL working concentration (typical working concentration used in most applications is 0.1µg/mL-10µg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.Biochem/physiol ActionsCell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.Features and Benefits- Ready-to-use liquid, cell permeable dye.
- Can be used with live cells and fixed cells.
- Effective for fixed-cell staining and quantitation of DNA content.
- Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.
Physical formDAPI readymade solution is provided at 1mg/mL concentration in waterПараметрыform liquid storage condition protect from light concentration 1 mg/mL application(s) transfection: suitable fluorescence ex 340 nm; em 488 nm (nur DAPI), ex 364 nm; em 454 nm (DAPI-DNA-Komplex) shipped in dry ice storage temp. 20°C SMILES string Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N InChI 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H InChI key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Safety Informationpictograms GHS07 signalword Warning hcodes H317 pcodes P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
DAPI ready made solution with Antifade MBD0020-10ML
Empirical Formula (Hill Notation): C16H15N5 · 2HCl CAS Number: 28718-90-3 Molecular Weight: 350.25 Beilstein/REAXYS Number: 4894417 MDL number: MFCD00012681 General descriptionDAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively. Most of the fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the composition of the molecule and the time of light exposure. DAPI Ready Made Solution with Antifade is a formulation that suppress photobleaching and prolong fluorescent signals.ApplicationDAPI Ready Made Solution with Antifade is a glycerol based ready to use solution provided at 1μg/mL concentration in a dropper vial that can be applied directly to cells or tissue samples on microscope slides or coverslips. It is recommended to warm up at room temperature and mix by swirling the dropper vial before use. Store DAPI Ready Made Solution with antifade at -20 C protected from light.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.Biochem/physiol ActionsCell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.Features and Benefits- Protects dyes from fading during imaging.
- Ready-to-use liquid.
- Easy to use Dropper vial application.
- Stained sample are stable for longer time period.
- Maintains fluorescence signal-little to no quenching.
Physical formLiquid - DAPI readymade with antifade solution is provided at 1μg/mL concentration in glycerol-PBS based solution.Параметрыform liquid storage condition protect from light application(s) transfection: suitable fluorescence ex 340 nm; em 488 nm (nur DAPI), ex 364 nm; em 454 nm (DAPI-DNA-Komplex) shipped in dry ice storage temp. 20°C SMILES string Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N InChI 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H InChI key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Safety Informationhcodes H412 pcodes P273 - P501 WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Escort™ III Transfection Reagent
NACRES: NA.25
Кат. номер L3037-1ML L3037-1ML-PW L3037-SAMPLE-PW L3037-SAMPLE General descriptionEscort™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.ApplicationSuitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 µl Escort™ III and 2 µg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort™ III:
A549
C2C12 myotubes
Cardiomyocytes (rat)
COS-7
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
HepG2
HeLa
Jurkat
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
NIH3T3
PC-12
Retinal Neurons (rat)
Tracheobronchial cells (sheep)Features and Benefits- Suitable for stable and transient transfection
- Optimized for a wide variety of primary cells
- Low toxicity
- Compatible with both serum and serum-free transfection protocols
- Ideal for PC-12 cells
ComponentsEscort™ III formulation:
1 mg/mL total lipid in water
Note the identity of the lipids used in Escort™ III is confidential.CautionDo not freeze.PrincipleA stable complex is formed when Escort™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.Legal InformationEscort is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 grade for molecular biology form liquid (aqueous solution) usage 1 mL sufficient for 250-1000 transfections concentration 1 mg/mL application(s) transfection: suitable storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Escort™ IV Transfection Reagent
NACRES: NA.25
Кат. номер L3287-1ML L3287-SAMPLE-PW L3287-1ML-PW L3287-SAMPLE General descriptionEscort™ IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.ApplicationSuitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 µL Escort™ IV and 2 µg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort™ IV, see the Transfection Reagent Selection Guide.Features and Benefits- Suitable for stable and transient transfection
- Optimized for a wide variety of cell lines
- Low toxicity
- Compatible with both serum and serum-free transfection protocols
- Ideal for Sf9, Sf21 and S2 insect cells
ComponentsEscort™ IV formulation:
1 mg/mL total lipid in water
Note the identity of the lipids used in Escort™ IV is confidential.CautionDo not freeze.PrincipleA stable complex is formed when Escort™ IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.Legal InformationEscort is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 100 grade for molecular biology form liquid (aqueous solution) usage 1 mL sufficient for 160-500 transfections concentration 1 mg/mL application(s) transfection: suitable storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
NeuroPorter™ Transfection Kit
NACRES: NA.25
Кат. номер NPT01-1KT NPT01-1KT-PW General descriptionNeuroporter™ Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons, glial cells, and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter™ Transfection Kit was designed for difficult-to-transfect primary neurons, addressing past problems such as poor cell viability, low transfection efficiency and neuro-degeneration.ApplicationSuitable for transient and stable transfection of nucleic acids into primary neurons and cultured neuronal cell lines. Use approximately 15-120 µl Neuroporter™ Transfection Reagent and 6-8 µg DNA (in provided unique DNA Dilution buffer when required) per 6 cm cell culture plate. The following cells have been successfully transfected using the Neuroporter™ Transfection Kit:- C6 glioma (human)
- Cortical neurons (rat primary)
- Dorsal Root Ganglion (DRG) cells (rat)
- NT2 neurons(human precursor cells)
- NT neurons (human differentiated cells)
- Subventricular Zone (SVZ) cells (mouse)
- White matter cells (mouse)
Features and Benefits- Optimized for primary neurons, glial cells, and cultured neural cell lines
- Very low toxicity with no neuro-degeneration or dendrite withdrawal
- Efficient DNA delivery primary neurons, glial cells, and cultured neural cell lines
- Fast and easy to use compared to other methods
- Compatible with both serum and serum-free transfection protocols
Components1 vial Neuroporter™ Transfection Reagent, dried lipid film (T2823)
1.5 mL Hydration Buffer H9036
7.5 mL DNA Diluent D1941CautionDo not freeze.PrincipleA stable, non-covalent complex is formed when the Neuroporter™ Transfection Reagent is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.Legal InformationNeuroPorter is a trademark of Gene Therapy Systems, Inc.ПараметрыQuality Level 200 grade for molecular biology form dried film usage 1 kit sufficient for 75-200 transfections availability available only in USA, Canada and EU application(s) transfection: suitable storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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