Subcellular Fractionation Reagents Sigma-Aldrich
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Chloroplast Isolation Kit
NACRES: NA.32
Кат. номер CPISO-1KT CPISO-1KT-PW General descriptionThe Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.ApplicationChloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.Features and Benefits- Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
- Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
- Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
- Convenient format - Saves time and reduces waste
SuitabilityThe Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.ПараметрыQuality Level 200 shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Endoplasmic Reticulum Isolation Kit
Кат. номер ER0100-1KT ER0100-1KT-PW General descriptionThe endoplasmic reticulum (ER) is a network of interconnected tubules, vesicles, and sacs. It plays a role in specialized cellular functions including protein synthesis, sequestering of calcium, production of steroids, storage and production of glycogen, and insertion of membrane proteins.ApplicationThe Endoplasmic Reticulum Isolation Kit contains all the reagents required for ER preparations with various degrees of purity: crude ER (microsomes), Ca2+ precipitated rough ER (RER) enriched microsomes, and density gradient purified rough ER (RER) and smooth ER (SER). The ER isolation process can be monitored by measuring the activity of NADPH cytochrome c reductase using the Cytochrome c Reductase Assay Kit, (Cat. No. CY0100). The enzyme is an ER membrane protein that is commonly used as an ER marker. The isolated ER can be used for studying the cytochrome P450 system and xenobiotic metabolism, for studying lipid metabolism, and for the recovery of ER membrane and lumenal proteins. The kit was tested with rat liver, kidney, and brain; mouse liver; rabbit liver, kidney, spleen, heart, and brain; and with Jurkat and HeLa cell lines.
The Endoplasmic Reticulum Isolation Kit is used for the efficient enrichment of functional endoplasmic reticulum (ER) from mammalian tissue or cell culture. The isolated ER are useful for protein synthesis, degradation and metabolic pathway analysis. Metabolic abnormalities of ER include carnitine acetyltransferase deficiency in children, cystic fibrosis and many more ER protein retention disorders. Suitable for up to 50 g of tissue or ~20 mL of packed tissue.Features and Benefits- Specially formulated extraction reagents for research scale applications - save time and minimize waste
- Includes a calcium chloride solution - for quick and simple precipitation of rough ER without need for ultracentrifugation
- Produces functional, intact organelles - resulting ER are suitable for functional studies, lipid metabolism, and protein profiling
- Compatible with products for structure confirmation - easily confirm intactness with companion kit test for cytochrome C reductase (CY0100)
- Can be used to isolate ER from soft animal tissues and cultured cells
ПараметрыQuality Level 200 storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Golgi Isolation Kit
NACRES: NA.32
Кат. номер GL0010-1KT GL0010-1KT-PW General descriptionThe Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).ApplicationGolgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.Analysis NoteThe Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.ПараметрыQuality Level 200 usage sufficient for 50 g (tissue) shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Lysosome Isolation Kit
EC Number: 233-140-8 NACRES: NA.32
Кат. номер LYSISO1-1KT-PW LYSISO1-1KT General descriptionLysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells. They contain many acid hydrolases that take part in protein degradation in the cell. Lysosomes also contain lipases, nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs, Gaucher, Hunter disease and others. They also contribute to maintaining cellular homeostasis. Malfunctions in this organelle directly impact cell behavior and fate. Lysosomes may also be involved in other cellular processes such as Albinism and aging.ApplicationLysosome Isolation Kit has been used:- in the isolation of cytosol and lysosome
- to obtain enriched light and heavy lysosomal fractions from cultured cells
- in the isolation of lysosomes of mouse bone marrow macrophages (BMMs)
- in lysosome purification
The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.Features and Benefits- Generates functional organelles for metabolism and disease research
- Includes protease inhibitor to manage degradation
- Measures integrity with included Neutral Red dye
- Choose from multiple options to obtain enriched fractions
- Enriched fractions for intact and functional lysosomes from tissues and cells
- Suitable for functional studies including protein degradation
ПараметрыQuality Level 200 usage sufficient for 20 mL (packed cells), sufficient for 25 g (tissue) storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H319 pcodes P264 - P280 - P305 + P351 + P338 - P337 + P313 RIDADR UN 1993 / PGIII Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Mammalian Cell Lysis Kit
NACRES: NA.56
Кат. номер MCL1-1KT MCL1-1KT-PW ApplicationThe product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.Features and Benefits- The kit can be used to prepare cell lysis buffer and other modified buffers
- The kit enables the researcher to change the buffers components to obtain maximum efficiency for a specific protein
ComponentsThe mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions.ПараметрыQuality Level 200 usage 1 kit sufficient for 250 extractions (100 mm tissue culture plates) shipped in dry ice storage temp. −20°C
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H302 - H315 - H318 - H412 pcodes P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 RIDADR NA 1993 / PGIII WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Mitochondria Isolation Kit
NACRES: NA.32
Кат. номер MITOISO2-1KT MITOISO2-1KT-PW ApplicationMitochondria Isolation Kit has been used in the isolation of mitochondria from Ewing′s Sarcoma Family of Tumor (ESFT) cells, blood samples from tuberculosis patients, human neuroblastoma cells and glioma cells, myotubes and HepG2 cells.
The Mitochondria Isolation Kit provides a fast and easy method for the isolation of an enriched mitochondrial fraction from cells. Most of the isolated mitochondria will contain inner and outer membranes. The kit contains extraction buffers for both functional studies and proteome profiling. Also included is a storage buffer for intact mitochondria.ПараметрыQuality Level 200 usage sufficient for 50 applications (2-5 x 107 cells) shipped in dry ice storage temp. 20°C
Safety Informationpictograms GHS07,GHS08,GHS09 signalword Warning hcodes H302 - H351 - H361fd - H411 pcodes P202 - P264 - P270 - P273 - P301 + P312 - P308 + P313 RIDADR UN 3316 9 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Peroxisome Isolation Kit
NACRES: NA.32
Кат. номер PEROX1-1KT PEROX1-1KT-PW General descriptionIsolated peroxisomes are used for studying lipid β-oxidation, amino acid metabolism and biosynthesis of ether-linked glycerolipids and bile acids.ApplicationThe Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.Features and Benefits- Specially formulated extraction reagents for research scale applications - save time and minimize waste
- Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
- Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)
Other NotesUpon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.Legal InformationOptiPrep is a trademark of Alere Technologies ASПараметрыQuality Level 200 shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 pcodes P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 RIDADR UN 1993 / PGIII WGK Germany WGK 2 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
ProteoPrep® Universal Extraction Kit PROTTWO-1KT
NACRES: NA.32 ApplicationThis kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis.- Fraction 1: Soluble/Cytoplasmic Proteins
- Fraction 2: Membrane Proteins
Features and Benefits- Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels.
- Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis.
- Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
- Pre-weighed dry blends - Stable and easy to reconstitute
- Conveniently Packaged - No waste. Use only the amount needed.
Legal InformationProteoPrep is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 form dry powder storage temp. 2-8°C
Safety Informationpictograms GHS02,GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H251 - H301 - H314 - H317 - H335 - H351 - H360FD - H411 pcodes P235 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Respiratory system RIDADR UN 3316 9 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Yeast Mitochondria Isolation Kit
NACRES: NA.32
Кат. номер MITOISO3-1KT MITOISO3-1KT-PW General descriptionMitochondria, the site of most of the energy production in eukaryotic cells, are characterized by a double membrane structure, an outer membrane and folded inner membrane. Isolated mitochondria may be used as tools for in vitro studies such as respiration and energy production studies, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling.ApplicationThe kit contains all the reagents required for yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation. In addition, the kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies and a storage buffer for use with intact mitochondria. Yeast Mitochondria Isolation Kit has been used in the isolation and extraction of mitochondria.Features and Benefits• The kits features include speed, sensitivity, ease of use, and specificity
• The kit contains all the reagents required for a fast and easy yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation
• The kit provides two alternatives for cell membrane disruption. The first one by a detergent and second one by homogenization.
• The kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies
• The kit includes a storage buffer for use with intact mitochondria.
• The reagents are sufficient for 40 procedures using 20 OD culture preparations
• The kit was tested on Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombeПараметрыQuality Level 200 usage sufficient for 40 applications (using 20 OD culture preparations) storage temp. −20°C
Safety Informationpictograms GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H301 + H331 - H312 - H315 - H318 - H334 - H351 - H361fd - H411 pcodes P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P311 - P305 + P351 + P338 RIDADR UN 3316 9 Узнать цену
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