Stem Cells Sigma-Aldrich

Medium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.

Quality Level	100
biological source	human dermis (fibroblast)
reprogramming method	episomal
description	age (15-19)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi010-B 66540030-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank, is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-81(+)POU5F1 (OCT-4)(+)TRA 1-60(+)DNMT3B(+)SOX2 (+)SSEA-3(+)NANOG(+)SSEA-4(+)
Differentiation potency
Ectoderm: Neuron
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed:Nestin(+)Tuj1(+)vGLUT1(+)VIMENTIN(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:SMA(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 18
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C

biological source	human dermis (fibroblast)
Quality Level	100,
reprogramming method	episomal
description	age (15-19)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi010-C 66540023-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-81 (+)TRA 1-60 (+)DNMT3B (+)POU5F1 (OCT-4) (+) NANOG (+)SOX2 (+)SSEA-3 (+) SSEA-4 (+)
Differentiation potency
Ectoderm: Neuron
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed:Nestin(+)Tuj1(+)vGLUT1(+)VIMENTIN (+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:SMA(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 17
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C

Quality Level	100
biological source	human dermis (fibroblast)
reprogramming method	episomal
description	age (15-19)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi010-C-10 66541074-1VL

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: P11
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: MODY
Type of modification: Isogenic
Gene: HNF1A
Chromosome location: 12q24.31
Nucleotide sequence HGSV: NM_000545.6:c.(c.872dup)
Protein sequence HGSV: NP_000536.5:p.(P291fsinsC)
Zygosity: Heterozygous
Target locus modification: Mutated
Description: An additional cytocine has been introduced by CRISPR-Cas9 into a poly-C tract in Exon 4 of HNF1A resulting in a out of frame mutation. It is likely that the other allele has also been targeted, since western blotting did not show any HNF1A protein in Pancreatic progenitor cells compared to the control line BIONi010-C.

Note: EAUA and CLIP must be completed before order fulfillment

Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage Number banked (pre-EBiSC): 12
No. Vials banked (pre-EBiSC): 4

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi010-C-11 66541075-1VL

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: MODY
Type of modification: Isogenic
Gene: HNF1A
Chromosome location: 12q24.31
Nucleotide sequence HGSV: NM_000545.6:c.(c.872dup)
Protein sequence HGSV: NP_000536.5:p.(P291fsinsC)
Zygosity: Homozygous
Target locus modification: Mutated
Description: An additional cytocine has been introduced by CRISPR-Cas9 into a poly-C tract in both alleles of Exon 4 of HNF1A resulting in a out of frame mutation. Western blotting did not show any HNF1A protein in Pancreatic progenitor cells compared to the control line BIONi010-C.

Note: EAUA and CLIP must be completed before order fulfillment

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi010-C-12 66541076-1VL

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative

Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: MODY
Type of modification: Isogenic
Gene: HNF4A
Chromosome location: 20q13.12
Nucleotide sequence HGSV: NM_175914.4:c.925C>T
Protein sequence HGSV: NP_787110.2:p.(p.Arg309Cys)
Zygosity: Homozygous
Target locus modification: Mutated
Description: A Cytocine to Tyrosine mutation was introduced into both alleles of exon 8 in HNF4A resulting in a mutation previously shown to cause MODY1 in patients.

Note: EAUA and CLIP must be completed before order fulfillment

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi010-C-13 66540561-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Note: EAUA and CLIP must be completed before order fulfillment

Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 18%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 33
No. Vials banked (pre-EBiSC): 20

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi010-C-15 66540570-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Note: EAUA and CLIP must be completed before order fulfillment

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi010-C-17 66540632-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Morphology:
TREM2 KO cl. 24-54 D1 thaw aft_ekU7aDe.tif - Phase contrast image 1 day after thawing
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 ecto D5 10x.tiff
Protocol or reference:
BIONi010-C-17 ecto D5.tif - Flow cytometry of in vitro differentiation to ectoderm
Endoderm:
Endoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 endo D5 10x.tiff
Protocol or reference:
BIONi010-C-17 endo D5.tif - Flow cytometry of in vitro differentiation to endoderm
Mesoderm:
Mesoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 meso D5 10x.tiff
Protocol or reference:
BIONi010-C-17 meso D5.tif - Flow cytometry of in vitro differentiation to mesoderm
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 35
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Image / Result

Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Gene knock-out
Gene: TREM2
Chromosome location: KO of exon 1 of TREM2 Transcript variant 1 NM_018965
Delivery method: CRISPR-associated (CRISPR/Cas) System

Note: EAUA and CLIP must be completed before order fulfillment

Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 35
No. Vials banked (pre-EBiSC): 30

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi010-C-18 66540637-1VL

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Morphology:
TREM2 KO cl. 24-54 D1 thaw aft_c8jzWGO.tif - Morphology 1 day after thawing
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Morphology:
BIONi10-C18 ecto d5 10x.tiff - Morphology of in vitro diff to ectoderm
Protocol or reference:
BIONi10-C18 ecto d5.tiff - Flow Cytometry of in vitro differentiation to ectoderm
Endoderm:
Endoderm
In vitro directed differentiation
Morphology:
BIONi10-C18 endo d5 10x.tiff - Morphology of in vitro diff to endoderm
Protocol or reference:
BIONi10-C18 endo d5.tiff - Flow Cytometry of in vitro differentiation to endoderm
Mesoderm:
Mesoderm
In vitro directed differentiation
Morphology:
BIONi10-C18 meso d5 10x.tiff - Morphology of in vitro diff to mesoderm
Protocol or reference:
BIONi10-C18 meso d5.tiff - Flow Cytometry of in vitro differentiation to mesoderm
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 35
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Image / Result

Note: EAUA and CLIP must be completed before order fulfillment

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi010-C-3 66540269-1VL

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Other: from the same donor: BIONi010-A, BIONi010-B
Characterization
Differentiation potency
Ectoderm: HES5 (+),NeuroD1 (+),PAX6 (+),Endoderm: CXCR4 (+),GSC (+),PITX1 (+),Mesoderm: DCN(+),NCAM1 (+),VIM(+)
Microbiology / Virus Screening
HIV 1:Negative
HIV 2:Negative
Hepatitis B:Negative
Hepatitis C: Negative
Mycoplasma:Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 31
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Gene knock-out
Gene: APOE
Chromosome location: 19q13.32
Delivery method: CRISPR-associated (CRISPR/Cas) System
Description: The ApoE gene is knocked out in this line; deletion of the ApoE gene by insertion of a frame shift in exon 1. The target locus has been mutated.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: -
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 18%
Temperature: 37°C

EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.

biological source	human dermis (fibroblast)
Quality Level	100,
reprogramming method	episomal
description	age (15-19)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi010-C-4 66540366-1VL

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Other:From the same donor: BIONi010-A, BIONi010-B
Characterization
Differentiation potency:Ectoderm: HES5 (+),NeuroD1 (+),PAX6 (+),Endoderm: CXCR4 (+),FOXA2 (+),GATA6 (+),GSC (+),PITX1 (+),SOX17 (+),Mesoderm: DCN (+),NCAM1 (+),VIM (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C:Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 28
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Isogenic
Chromosome location: 19q13.32
Nucleotide sequence HGSV: rs7412 (C/C); rs429358 (C/C)
Protein sequence HGSV: 112Arg, 158Arg
Zygosity: Homozygous
Target locus modification: The bases at both SNPs rs7412 and rs429358 were changed to a C

Note: EAUA and CLIP must be completed before order fulfillment

Medium: -
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 18%
Temperature: 37°C

EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.

biological source	human dermis (fibroblast)
reprogramming method	episomal
description	age (15-19)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi010-C-5 66540367-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
Marker Expressed
HES5 Yes
NEUROD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
CXCR4 Yes
FOXA2 Yes
GATA6 Yes
GSC Yes
SOX17 Yes
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
Marker Expressed
DCN Yes
Vimentin Yes
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 27
Cell line karyotype: 46 XY
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Isogenic
Description: Bioneer to update this information when characterization has been completed. CD33 gene mutated; modified sequence CCTGCTGTGGGCAGgtgagtggctcgggggagcttgaccagaggttgatcttctct

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 18%
Temperature: 37°C

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi010-C-7 66540369-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Other:From the same donor: BIONi010-A, BIONi010-B
Characterization
Differentiation potency
Ectoderm: HES5(+),NeuroD1(+),PAX6 (+),Endoderm: CXCR4(+),FOXA2 (+),GATA6 (+),GSC (+)PITX1(+)SOX17 (+),Mesoderm: DCN(+)NCAM1 (+)VIM(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2:Negative
Hepatitis B: Negative
Hepatitis C:Negative
Mycoplasma:Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 27
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease: Alzheimers disease
Type of modification: Isogenic
Gene: TREM2
Chromosome location: 6p21.1
Nucleotide sequence HGSV: rs75932628(T)
Protein sequence HGSV: R47H
Zygosity: Homozygous
Target locus modification: Mutated

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 18%
Temperature: 37°C

biological source	human dermis (fibroblast)
reprogramming method	episomal
description	age (15-19)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi010-C-8 66540370-1VL

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Other:From the same donor:BIONi010-A, BIONi010-B
Characterization
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Isogenic
Chromosome location: 6p21.1
Protein sequence HGSV: NM_018965 T66M
Zygosity: Homozygous
Target locus modification: Mutated

Note: EAUA and CLIP must be completed before order fulfillment

biological source	human dermis (fibroblast)
reprogramming method	episomal
description	age (15-19)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi010-C-9 66540371-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Morphology:
03-30-2017 BionI010-C9.tif
Differentiation potency
Endoderm:
Cxcr4
Endoderm
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 25
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Gene knock-out
Chromosome location: 19q13.41
Delivery method: CRISPR-associated (CRISPR/Cas) System
Description: One base in exon one as well as 578 bases downstream (including whole exon 2) have been deleted. The basepair deletion leads to a frame shift. The larger deletion might not be necessary for a functional KO.

Note: EAUA and CLIP must be completed before order fulfillment

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi011-A 66540262-1VL

Derivation
No Information available
Characterization
No Information available
Genotyping
No Information available

Note: EAUA and CLIP must be completed before order fulfillment

No Information available

EBiSC is a trademark of Fraunhofer-Gesellschaft

biological source	human dermis (fibroblast)
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi012-A 66540263-1VL

Derivation
No Information available
Characterization
No Information available
Genotyping
No Information available

Note: EAUA and CLIP must be completed before order fulfillment

No Information available

EBiSC is a trademark of Fraunhofer-Gesellschaft

biological source	human dermis (fibroblast)
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi013-A 66540264-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control.
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: GABRB3(+)NANOG (+)DMNT3B(+)GDF3 (+)TRA 1-81 (+)SSEA-4 (+)TDG1(+)SSEA-1(-)POU5F1 (OCT-4)(+)POU5F1 (OCT-4) (+)SOX2(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed: Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi014-A 66540265-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control.
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: TDG1(+)TRA 1-81(+)POU5F1 (OCT-4)(+)SOX2(+)GDF3 (+)DMNT3B (+)GABRB3(+)POU5F1 (OCT-4)(+)NANOG(+)SSEA-4 (+)SSEA-1(-)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6 (+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46, XY normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
reprogramming method	episomal
description	age (25-29)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi015-A 66540266-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control.
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)TDG1 (+)SSEA-1(-)SSEA-4(+)TRA 1-81(+)SOX2 (+)DMNT3B(+GDF3(+)GABRB3(+)NANOG(+)POU5F1 (OCT-4) (+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17(+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi016-A 66540267-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control.
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: GDF3(+)NANOG(+)POU5F1 (OCT-4) (+)GABRB3(+)SSEA-1(-)SSEA-4 (+)TRA 1-81(+)DMNT3B (+)SOX2(+)TDG1(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin (+)PAX6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)SOX17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)NCAM1(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Cell line karyotype: 46,XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi017-A 66540360-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)TDG1(+)POU5F1 (OCT-4)(+)SSEA-1 (-)TRA 1-81 (+)SOX2 (+)GABRB3(+)GDF3(+) DMNT3B(+)NANOG(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin (+)Pax6 (+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184 (+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi018-A 66540361-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Primary cell developmental stage: Adult
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control.
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: GABRB3 (+)DMNT3B (+)POU5F1 (OCT-4) (+)GDF3 (+)NANOG (+)TDG1 (+) SSEA-4 (+)SSEA-4 (+)SSEA-1 (-)SOX2 (+)TRA 1-81 (+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin (+)Pax6 (+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184 (+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34 (+)CD56 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi019-A 66540362-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control.
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-1(-)SOX2(+)POU5F1 (OCT-4)(+)SSEA-4(+)TDG1(+)NANOG(+)TRA 1-81(+)DMNT3B (+)GABRB3(+)GDF3(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:estin (+)Pax6 (+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi020-A 66540363-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: DMNT3B(+)POU5F1 (OCT-4)(+)SOX2 (+)GDF3(+)SSEA-4 (+)TDG1(+)TRA 1-81(+)SSEA-1(-)GABRB3(+)NANOG(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 16
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi021-A 66540364-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-81(+)GABRB3(+)SSEA-4(+)DMNT3B(+)POU5F1 (OCT-4)(+)NANOG(+)SSEA-1(-)TDG1 (+)GDF3(+)SOX2(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi022-A 66540365-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GABRB3 Yes
SOX2 Yes
TDG1 Yes
SSEA-1 No
POU5F1 (OCT-4) Yes
SSEA-4 Yes
TRA 1-81 Yes
GDF3 Yes
NANOG Yes
DMNT3B Yes
GABRB3 Yes
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Pax6 Yes
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
CD184 Yes
Sox17 Yes
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
CD34 Yes
CD56 Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi023-A 66540372-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Location of primary tissue procurement: Adipose tissue
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GDF3 Yes
TDG1 Yes
GABRB3 Yes
NANOG Yes
DMNT3B Yes
SSEA-4 Yes
TRA 1-81 Yes
SOX2 Yes
SSEA-1 No
POU5F1 (OCT-4) Yes - -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Pax6 Yes
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
CD184 Yes
Sox17 Yes
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
CD34 Yes
CD56 Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 14
Cell line karyotype: 46, XY, normal male karyotype
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi024-A 66540373-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Location of primary tissue procurement: Adipose tissue
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4 Yes
SSEA-1 No
GDF3 Yes
POU5F1 (OCT-4) Yes - -
SOX2 Yes
GABRB3 Yes
DMNT3B Yes
NANOG Yes
TRA 1-81 Yes
TDG1 Yes
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Pax6 Yes
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
CD184 Yes
Sox17 Yes
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
CD34 Yes
CD56 Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Cell line karyotype: 46, XY, normal male karyotype
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi025-A 66540374-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Location of primary tissue procurement: Adipose tissue
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4) Yes - -
GABRB3 Yes
GDF3 Yes
NANOG Yes
SOX2 Yes
DMNT3B Yes
SSEA-4 Yes
TRA 1-81 Yes
SSEA-1 No
TDG1 Yes
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Pax6 Yes
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
CD184 Yes
Sox17 Yes
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
CD34 Yes
CD56 Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46,XY, normal male karyotype
Karyotyping method: G-Banding
Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi026-A 66540399-1VL

Note: EAUA and CLIP must be completed before order fulfillment

EBiSC is a trademark of Fraunhofer-Gesellschaft

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi027-A 66540400-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-81(+)SSEA-1(-)GABRB3(+)DMNT3B(+)SSEA-4(+)TDG1(+)POU5F1 (OCT-4)(+)SOX2(+)NANOG(+)GDF3(+)
Differentiation potency
Ectoderm:
Endoderm:
Endoderm:In vitro directed differentiation
Marker Expressed:CD184(+)Sox17(+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 14
Cell line karyotype: 46, XY normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi028-A 66540401-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SOX2(+)TRA 1-81(+)SSEA-1(-)TDG1(+)POU5F1 (OCT-4)(+)NANOG(+)DMNT3B(+)GDF3(+)GABRB3(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34 (+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi029-A 66540402-1VL

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi030-A 66540403-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)GABRB3(+)TRA 1-81(+)SOX2(+)POU5F1 (OCT-4)(+)SSEA-1(-)NANOG (+)GDF3(+)TDG1(+)DMNT3B (+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17(+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 14
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi031-A 66540404-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: DMNT3B(+)SSEA-4(+)GDF3(+)SSEA-1(-)POU5F1 (OCT-4)(+)NANOG(+)TDG1(+)TRA 1-81(+)SOX2(+)GABRB3(+)
Morphology:
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 14
Cell line karyotype: 46,XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi032-A 66540405-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: Unknown
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: GDF3(+)GABRB3(+)DMNT3B(+)TDG1 (+)SSEA-1(-)SSEA-4(+)TRA 1-81(+)SOX2(+)POU5F1 (OCT-4)(+)NANOG(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 13
Cell line karyotype: 46,XY, normal male karyotype
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi033-A 66540423-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)SSEA-1(-)GDF3(+)SOX2(+)DMNT3B(+)GABRB3(+)NANOG(+)POU5F1 (OCT-4)(+)TRA 1-81 (+) TDG1(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17(+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 14
Cell line karyotype: 46,XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi034-A 66540424-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: DMNT3B(+)GDF3(+)GABRB3(+)NANOG(+)SSEA-1(-)SSEA-4(+)POU5F1 (OCT-4)(+)TRA 1-81(+)SOX2(+)TDG1(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6 (+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184(+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34 (+)CD56(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 14
Cell line karyotype: 46,XY, normal male karyotype
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi035-A 66540425-1VL

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: TDG1(+)SSEA-1(-)TRA 1-81(+)POU5F1 (OCT-4)(+)NANOG(+)SSEA-4(+)SOX2(+)DMNT3B(+)GABRB3(+)GDF3(+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin(+)Pax6(+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184 (+)Sox17(+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34(+)CD56 (+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 19
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: Essential E8®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

biological source	human adipose-derived MSC
Quality Level	100,
reprogramming method	episomal
description	age (20-24)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi037-A 66540580-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Derivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2010
Passage number reprogrammed: 18
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Morphology:
07-25-2017 16423 apoe 33 .tif
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 25
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Image / Result

Note: EAUA and CLIP must be completed before order fulfillment

biological source	human dermis (fibroblast)
reprogramming method	episomal
description	age (75-79)
Торговая марка	EBiSC^™
gender	female
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	none
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi037-A-1 66540581-1VL

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: refer to BIONi037-A
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Morphology:
02-06-2017 bioni037-a-1 apoe ko 16423.tif
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Cell line karyotype: 23
Karyotyping method: G-Banding
Image / Result

Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Gene knock-out
Gene: Apolipoprotein E
Chromosome location: 19q13.32
Delivery method: CRISPR-associated (CRISPR/Cas) System
Description: WB analyses reveals a knockout of the APOE gene

Note: EAUA and CLIP must be completed before order fulfillment

Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
Culture medium: Essential E8®
No. Vials banked (pre-EBiSC): 30

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	female
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi037-A-2 66540604-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Morphology:
03-08-2017 16423 apoe 22.tif
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Isogenic
Chromosome location: 19q13.32
Nucleotide sequence HGSV: rs7412 (T/T); rs429358 (T/T)
Protein sequence HGSV: 112Cys, 158Cys
Zygosity: Homozygous
Target locus modification: The bases at both SNPs rs7412 and rs429358 were changed to a T

Note: EAUA and CLIP must be completed before order fulfillment

Surface coating: Matrigel®/Geltrex®
Culture medium: Essential E8®

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	female
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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BIONi037-A-3 66540605-1VL

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Isogenic
Chromosome location: 19q13.32
Nucleotide sequence HGSV: rs7412 (C/C); rs429358 (C/T)
Zygosity: Heterozygous
Target locus modification: The bases SNPs rs7412 have been changed to a C and at rs429358 to a T and a C

Note: EAUA and CLIP must be completed before order fulfillment

Culture medium: Essential E8®
No. Vials banked (pre-EBiSC): 30

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	female
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BIONi037-A-4 66540606-1VL

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntingtons Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative

Genotyping
Karyotyping
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Isogenic
Gene: Apolipoprotein E
Chromosome location: 19q13.32
Nucleotide sequence HGSV: rs7412 (C/C); rs429358 (C/C)
Zygosity: Homozygous
Target locus modification: The bases at both SNPs rs7412 and rs429358 were changed to a C

Note: EAUA and CLIP must be completed before order fulfillment

Culture medium: Essential E8®
No. Vials banked (pre-EBiSC): 30

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft

biological source	human
reprogramming method	episomal
description	age (N/A)
Торговая марка	EBiSC^™
gender	female
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	Alzheimers disease
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

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CENSOi001-B 66540599-1VL

Depositor
Censo Biotechnologies

Derivation
Primary cell type: Fibroblast
Tissue collection year: 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: None
Vector Free Reprogramming
Types of factor(s) used: mRNA
mRNA: NANOG
LIN28
MYC
KLF4
SOX2
POU5F1
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes
POU5F1 (OCT-4) Yes
SSEA-4 Yes
SSEA-1 No
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
HES5 Yes
NeuroD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
CXCR4 Yes
GATA6 Yes
SOX17 Yes
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
MIXL1 Yes
NCAM1 Yes
VIM Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 12
Cell line karyotype: 46,XY
Karyotyping method: G-Banding
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C

biological source	human dermis (fibroblast)
reprogramming method	mRNA
description	age (5-9)
Торговая марка	EBiSC^™
gender	male
growth mode	adherent (pluripotent)
application(s)	cell culture \| stem cell: suitable
relevant disease(s)	muscular dystrophy (Duchenne)
shipped in	dry ice
storage temp.	196°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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CENSOi002-B 66540597-1VL

Depositor
Censo Biotechnologies

Derivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: None
Vector Free Reprogramming
Types of factor(s) used: mRNA
mRNA: NANOG
LIN28
MYC
KLF4
SOX2
POU5F1
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes
SSEA-1 No
POU5F1 (OCT-4) Yes
SSEA-4 Yes
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
HES5 Yes
NeuroD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
CXCR4 Yes
GATA6 Yes
SOX17 Yes
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
MIXL1 Yes
NCAM1 Yes
VIM Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 9
Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected
Karyotyping method: KaryoLite BoBs
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Note: EAUA and CLIP must be completed before order fulfillment

Medium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C