Recombinant Trypsin

NACRES:

NA.26

Кат. номер

EMS0006-4X100UG

EMS0006-PH

EMS0006-100UG

Trypsin is a major proteolytic enzyme, synthesized as a preproenzyme by pancreas and is stored as proenzyme trypsinogen in secretory granules. Trypsin belongs to the family of serine proteases that are characterized by the catalytic triad His57, Asp102 and Ser195. Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. Trypsin is a pancreatic serine endoprotease which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. The enzyme also exhibits esterase and amidase activities. Trypsin has an average molecular mass of 23.29 kDa and a pH optimum near 8.0. This product is prepared from recombinant trypsin, porcine sequence. It is naturally devoid of chymotryptic activity. This high-quality trypsin is suitable for proteomics use.
Biochem/physiol Actions
Trypsin plays an important role in the digestion of consumed protein and contributes to the activation of other proteolytic enzymes like chemotrypsin and elastase.

Quality Level	200
recombinant	expressed in Pichia pastoris
assay	10,000 unit/mg protein
form	lyophilized powder
suitability	suitable for ","suitable for mass spectrometry
UniProt accession no.	P00761,
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS08,GHS09
signalword	Danger
hcodes	H290 - H314 - H317 - H332 - H334 - H335 - H411
pcodes	P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Target Organs	Respiratory system
Supp Hazards	EUH071
RIDADR	UN 1789 8 / PGIII
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable