Protein Electrophoresis Gel Stains Sigma-Aldrich
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EZBlue™ Gel Staining Reagent
NACRES: NA.32
Кат. номер G1041-500ML G1041-3.8L General descriptionConvenient, sensitive, and safe, EZBlue™ Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.ApplicationEZBlue® Gel Staining Reagent has been used as a staining reagent in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).Features and Benefits- Premixed solution eliminates the time and effort required to prepare the stain
- Increased sensitivity ensures that low abundance proteins can be detected (as little as 5 ng)
- Rapid reaction significantly reduces the amount of time required to stain and rinse
- No solvent waste so you save time and money by eliminating hazardous material disposal
Other NotesA ready-to-use Brilliant Blue G-250 based protein stain for "one step" ultrasensitive detection on polyacrylamide gels and PVDF membranes.Legal InformationEZBlue is a trademark of Sigma-Aldrich Co. LLC
Sigma-Aldrich is a registered trademark of Sigma-Aldrich Co. LLCПараметрыapplication(s) protein staining: suitable Quality Level 200 storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS08 signalword Warning hcodes H290 - H315 - H319 - H371 pcodes P234 - P260 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P311 Personal Protective Equipment Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter RIDADR UN 3264 8 / PGII WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Fixing solution
Кат. номер F7264-500ML F7264-1L ApplicationAn effective protein fixing solution for pre-stain fixing, suitable for SDS-PAGE, non-denaturing PAGE and IEF. Proteins require fixing into the gel for analytical purposes so that they do not leach out following electrophoresis.Packaging1 L in poly bottleOther Notes60% (w/v) trichloroacetic acid, 17.5% (w/v) 5-sulfosalicylic acid.ПараметрыQuality Level 200 concentration 5 x (solution) application(s) protein staining: suitable
Safety Informationpictograms GHS05,GHS07,GHS09 signalword Danger hcodes H314 - H335 - H410 pcodes P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P391 Target Organs Respiratory system RIDADR UN 2564 8 / PGII WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Naphthol Blue Black
Empirical Formula (Hill Notation): C22H14N6Na2O9S2 CAS Number: 1064-48-8 Molecular Weight: 616.49 Colour Index Number: 20470 Beilstein/REAXYS Number: 5374263 EC Number: 213-903-1 MDL number: MFCD00004017 PubChem Substance ID: 24897642 NACRES: NA.32
Кат. номер N3393-25G N3393-100G ApplicationNaphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.Packaging25, 100 g in glass bottleBiochem/physiol ActionsNaphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.ПараметрыQuality Level 200 product line BioReagent composition Dye content, ~85% application(s) protein staining: suitable impurities ≤10% water suitability suitable for electrophoresis SMILES string [Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O InChI 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;; InChI key AOMZHDJXSYHPKS-DROYEMJCSA-L
Safety InformationPersonal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
ProteoSilver™ Plus Silver Stain Kit PROTSIL2-1KT
NACRES: NA.32 General descriptionConveniently packaged, extremely stable, and highly sensitive, ProteoSilver™ Plus Silver Stain Kit is an ideal product for any protein scientist. Similar to ProteoSilver (PROTSIL1), this kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver Plus leads to superior detection of low abundance proteins.
ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.ApplicationProteoSilver® Silver Stain Kit has been used for staining of the gels for visualizing proteins. It has also been used for destaining of the protein of interest using destainer provided in the kit for mass spectroscopic analysis.Features and Benefits- Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
- Optimized protocols help establish conditions for best results
- High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
- MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
- Room Temperature Stability allows easy and convenient storage
Legal InformationProteoSilver is a trademark of Sigma-Aldrich Co. LLC
Sigma-Aldrich is a registered trademark of Sigma-Aldrich Co. LLCПараметрыusage 1 kit sufficient for 25 mini-gels (10 x 10 cm) Quality Level 200 application(s) protein staining: suitable storage temp. 20-25°C
Safety Informationpictograms GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H301 + H311 + H331 - H314 - H317 - H335 - H341 - H350 - H371 - H410 pcodes P201 - P273 - P280 - P301 + P310 + P330 - P303 + P361 + P353 - P305 + P351 + P338 + P310 Target Organs Eyes, Respiratory system Supp Hazards EUH032 RIDADR UN 3316 9 WGK Germany WGK 3 Flash Point F 149.0 °F - closed cup Flash Point C 65 °C - closed cup Узнать цену -
ProteoSilver™ Silver Stain Kit PROTSIL1-1KT
General descriptionConveniently packaged, extremely stable, and highly sensitive, ProteoSilver is an ideal product for any protein scientist. The kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver leads to superior detection of low abundance proteins.ApplicationProteoSilver™ Silver Stain Kit has been used for staining of the gels for visualizing proteins.Features and Benefits- Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
- Optimized protocols help establish conditions for best results
- High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
- MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
- Room Temperature Stability allows easy and convenient storage
Legal InformationProteoSilver is a trademark of Sigma-Aldrich Co. LLCПараметрыusage 1 kit sufficient for 25 mini-gels (10 x 10 cm) Quality Level 200 application(s) protein staining: suitable
Safety Informationpictograms GHS02,GHS03,GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H226 - H272 - H290 - H301 + H311 + H331 - H314 - H317 - H335 - H341 - H350 - H371 - H410 pcodes P201 - P210 - P280 - P301 + P310 + P330 - P303 + P361 + P353 - P305 + P351 + P338 + P310 Target Organs Eyes, Respiratory system RIDADR UN 3316 9 WGK Germany WGK 3 Flash Point F 132.8 °F - closed cup Flash Point C 56 °C - closed cup Узнать цену -
READYBLUE™ PROTEIN GEL STAIN
Кат. номер RSB-30ML RSB-1L General descriptionReadyBlue™ Protein Gel Stain is a rapid and sensitive colloidal Coomassie stain for polyacrylamide gels. The stain is provided as a ready-to-use solution, allowing for a fast and simplified protocol. No prior fixing step is necessary.- Colorimetric or IR fluorescence (800 nm) imaging
- Reusable up to 3 times
- No organic solvents or phosphoric acid
- Compatible with mass spectrometry
- Room temperature storage—its ready when you are
ApplicationProtein gel stainStorage and StabilityStore ReadyBlue™ Protein Gel Stain at room temperature. Protect From Light.Legal InformationREADYBLUE is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 100 quality MQ100 application(s) protein staining: suitable Узнать цену -
Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels RPROB-1KT
NACRES: NA.32 ApplicationR-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.Other NotesCompatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.Параметрыusage sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels) Quality Level 200 application(s) protein staining: suitable storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 pcodes P305 + P351 + P338 RIDADR NONH for all modes of transport Узнать цену
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