Protease Profiler™ Kit PP0500-1KT

NACRES:

NA.56

For detailed characterization of proteins of interest, increase your protease selection with the Protease Profiler. The Protease Profiler provides four proven alternative proteases in addition to Proteomics Grade Trypsin, an enzyme solublization reagent, and an enzyme reaction buffer. Each component has been stringently purified and qualified for MALDI-MS to ensure optimal performance. Characterize with greater flexibility, perform double enzymatic digestions, and explore alternative cleavage sites with the Protease Profiler.

• Double Enzymatic Digestion - An initial digestion may result in peptide fragments greater than 5 kDa, often above the optimum detection range for most MS systems/instruments. A second digestion using a protease with different specificity results in cleavage of the large peptides, generating smaller peptides suitable for MS.
• Explore Alternative Proteases - Trypsin may be incompatible with some protein samples. Find the protease best suited to your protein of interest and improve the quality of your data.
• Perform Either Solution or Gel-based Digests - Optimized protocols and reagents for both digest conditions are provided, enabling flexibility in your analysis.

The Protease Profiler™ Kit can be used for different enzymatic digestions on the proteins of interest.
Storage and Stability
The entire Protease Profiler™ Kit retains activity for at least 1 year when stored at –20 °C. Alternatively, some of the components (Trypsin, Lys-C, Asp-N, Trypsin Solubilization Reagent, and Enzyme Reaction Buffer) may be stored at 2–8 °C. The storage temperature indicated on the labels of these products is 2–8 °C. Storage at –20 °C will not compromise the performance of these products.
Protease Profiler is a trademark of Sigma-Aldrich Co. LLC

quality	Proteases for Mass Spectrometry and Proteomics
Quality Level	200,
storage temp.	20°C

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H302 - H315 - H317 - H319 - H334 - H335
pcodes	P261 - P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable