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Bovine Aortic Endothelial Cells: BAOEC (Cryovial) B304-05
NACRES: NA.81 General descriptionBovine large artery endothelial cells (BAOEC) provide an excellent model system to study all aspects of cardiovascular function and disease, such as critical signaling pathways and mechanisms relevant to proper function of endothelia, including angiogenesis, permeability, flow adaptation, NO production, diabetes-related complications, etc., and search for beneficial modulators and delivery systems for therapeutic use, and to design scaffolds, surfaces and materials for tissue engineering and 3D modeling.Cell Line OriginAortaApplicationcardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testingComponentsBovine Endothelial Cell Growth Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Bovine Endothelial Cell Growth Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the BAOEC Culture Protocol.Параметрыbiological source bovine aorta (USDA-inspected cattle) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 60 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Canine Aortic Endothelial Cells: CnAOEC (Cryovial) CN304-05
NACRES: NA.81 General descriptionCnAOEC provide a useful model to study cardiovascular diseases and test potential therapeutic agents, not only important from a veterinary perspective, but also having implications for human health. In a study aiming to adapt an immunomagnetic isolation protocol used in human patients to isolate circulating endothelial cells in dogs, CnAOEC cells were shown to be positive for von Willebrand factor, CD146 and stained with Ulex europaeus agglutinin 1 (Wills, 2009). To investigate the mechanism of N-terminal portion of pro C-type natriuretic peptide (NT-pCNP), a sepsis biomarker in humans and dogs, CnAOEC were treated with a variety of agents and it was found that lipopolysaccharide, TNF-α and IL-1β, but not IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A, stimulated NT-pCNP production (Osterbur, 2012, 2013). CnAOEC were also used to demonstrate vasoprotective activity of pomegranate and soy isoflavons in order to prevent endothelial disfunction in dogs which commonly leads to cardiovascular disease (Baumgartner-Parzer, 2012). Finally, CnAOEC were utilized as a control to compare the growth of normal canine endothelial cells and aggressive canine hemangiosarcoma cells in a study investigating signaling pathways underlying hemangiosarcoma oncogenesis (Murai, 2012), and as a VEGFR2-positive control in a study aimed at understanding the reasons for reduced numbers of circulating progenitor cells in cardiovascular disease (Boilson, 2010).Cell Line OriginAortaApplicationcardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testingComponentsCanine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Canine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the CnAOEC Culture Protocol.Параметрыbiological source canine aorta packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 78 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Canine Osteoblasts: CnOb (Cryovial) CN406-05
NACRES: NA.81 General descriptionCanine osteoblasts provide an excellent model system for studies relevant to the skeletal system, including mechanisms of cancerogenesis.
Canine osteoblasts have been used to define a biomarker profile of canine osteosarcoma (Milovancev, 2013), elucidate the roles of oncogenic STAT3 (Fossey, 2009, 2011) and canonical Wnt signaling (Piskun, 2013) pathways, and to develop personalized osteosarcoma treatments by testing Src inhibitor dasatinib (Davis, 2013), procaspase-3 activating compound PAC-1 (Book, 2012), HSP90 inhibitor STA-1474 (McCleese, 2009) and STAT3 inhibitor LLL12 (Couto, 2012, 2013).
Characterization: Positive for bone mineralization.Cell Line OriginBoneApplicationmodel for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatmentsComponentsCanine Osteoblast Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Canine Osteoblast Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the CnOb Culture Protocol.Параметрыbiological source canine bone (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 78 morphology osteoblast application(s) cell culture | mammalian: suitable relevant disease(s) arthritis; osteoporosis shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Canine Skeletal Muscle Cells: CnSkMC (Cryovial) CN150-05
NACRES: NA.81 General descriptionCanine Skeletal Muscle Cells (CnSkMC) provide a useful system to study many aspects of muscular function and disease and can serve as an intermediate model of human diseases. Skeletal Muscle Cells also play an instrumental role in the glucose metabolism and diabetes.Cell Line OriginMuscleApplicationlipid and glucose metabolism, exercise, insulin action, cell signalingComponentsCanine Skeletal Muscle Cell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Canine Skeletal Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 15 doublings
Subculture RoutinePlease refer to the CnSkMC Culture Protocol.Параметрыbiological source canine skeletal muscle (normal limb) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 78 morphology Skeletal muscle application(s) cell culture | mammalian: suitable relevant disease(s) muscular dystrophy shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Hair Follicle Dermal Papilla Cells: HFDPC, adult 602-05A
NACRES: NA.81 General descriptionHuman Hair Follicle Dermal Papilla Cells (HFDPC) are mesenchymal cells isolated from the hair papilla of normal human scalp hair follicles. Hair papilla, in the adult hair follicle, plays a crucial role in the dermal-epidermal interactions that control hair production and events of the hair growth cycle. HFDPC can therefore be used for studying cellular mechanisms organizing the hair follicle, and for the development and evaluation of hair growth products.
HFDPC have been used to demonstrate that IL-1 affects dermal papilla activity by decreasing androgen receptor expression and stimulating secretion of KFG, VEGF, IL-8 and GM-CSF (Boivin, 2006). They were also used to investigate how gene expression and cell proliferation are affected by testosterone analogues (Reiter, 2008), potential hair growth enhancing agents (Sasajima, 2008) and microbial metabolites (Takigawa, 2012). Using HFDPC, along with Human Epidermal Keratinocytes and Human Bronchial Epithelial Cells, scientists also studied mechanisms of cellular senescence, and develop ed methodology to extend cellular life span and immortalize the cells by inhibiting p16INK4a and introducing hTERT (Haga, 2007). Finally, HFDPC have been used to discover the link between the androgen-induced alopecia and circadian rhythms, mediated by a “clock gene” transcription factor BMAL1 (Watabe, 2013).Cell Line OriginScalpApplicationdermatalogical interactions, hair production, induction and maintenance of hair growth, hair follicle organization, development and evaluation of hair growth products, effects and secretion of growth factors and cytokines, gene expression, cell proliferation, cell senescence, cell lifespan extension, in vitro screening of androgen blocking agents, reservoir of multi-potent stem cells, skin reconstruction, wound healing, signal transduction pathways, skin composites and substitutes, hair follicle neogenesisComponentsCell Basal Medium containing 20% FBS & 5% DMSOPreparation Note- 2nd passage, >500,000 cells in Cell Basal Medium containing 20% FBS & 5% DMSO
- Can be cultured at least 6 doublings
Subculture RoutinePlease refer to the HFDPC Culture Protocol.Параметрыbiological source human scalp (hair follicle papilla) packaging pkg of 500,000 cells growth mode Adherent morphology mesenchymal application(s) cell culture | mammalian: suitable relevant disease(s) Alzheimer′s disease shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Aortic Endothelial Cells: HAOEC, adult 304-05A
NACRES: NA.81 General descriptionHuman Aortic Endothelial Cells (HAOEC) provide an excellent model system to study all aspects of cardiovascular function and disease, and they have been utilized in dozens of research publications to study diabetes-associated complications related to cardiovascular function, investigate mechanisms of immune response and graft rejection, study endothelial dysfunction caused by air pollution, oxidative stress and inflammation, and develop 3d endothelialized engineered tissues, as well as new technologies based on novel material surfaces and drugs in order to reduce risks associated with vascular implants.
Select HAOEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).
HAOEC from Cell Applications, Inc. have been used to:- demonstrate that increased glucose flux leads to endothelial dysfunction in diabetes via activating Egr1-mediated proinflammatory and prothrombotic responses (Vedantham, 2013);
- study apoptosis, oxidative stress and inflammation associated with atherosclerosis (Fu, 2014) and demonstrate the beneficial effects of anthocyanin on endothelial cells damaged by exposure to oxidized sterols (Wang, 2012);
- demonstrate that upregulation of thioredoxin via AMPK-FOXO3 pathway protects endothelial cells from oxidative stress and may prevent cardiovascular diseases in patients with metabolic syndrome and diabetes (Li, 2009a,b; Hou, 2010) and further elucidate the involvement of AMPK cascade in mediating beneficial cardiovascular effects of green tea (Reiter, 2010);
- test anti-inflammatory and vasodilating properties of a synthetic rutaecarpine derivative (Lee, 2013);
- show that glycated albumin, associated with diabetic complications, decreases endothelial miR-146a expression which leads to increased IL-6 production, and that angiotensin protects endothelial cells by preventing miR-146a downregulation (Wang, 2013);
- demonstrate that air pollutants can directly affect ZO-1 function leading to increased endothelial permeability, inflammatory cell transmigration and initiation of atherosclerosis (Li, 2010);
- discover the involvement of stress signaling JNK and p38 pathways in pathological suppression of thrombomodulin, a vascular protective molecule, downregulated in many thrombotic and vascular diseases (Rong, 2010);
- link uremic toxins (in particular, PAA) in patients with chronic liver disease to increased ROS production and stimulation of TNF-a in endothelial cells leading to atherosclerosis and vascular calcification (Morita, 2011);
- demonstrate that in diabetes, advanced glycation end products lead to ROS generation in endothelia via sustained NF-kB activation, contributing to progression of atherosclerosis (Morita, 2013);
- discover that CD40 ligand promotes monocyte adhesion to endothelial cells via PKCa, NF-kB and VCAM-1 signaling cascade, explaining the role of CD40L in atherogenesis (Wu, 2013);
- show that monocytes activated by endothelial cells, produce CD80 signaling that leads to allogenic immune response, indicating the need for specific therapy to prevent monocyte activation during allograft transplantation (Wang, 2008);
- identify tetraspanin CD82 as the recognition sensor responsible for rejection of xenotransplants (Saleh, 2013);
- develop 3d endothelialized engineered tissues (Sakai, 2012), as well as new technology based on novel material surfaces and drugs (such as paclitaxel, sirolimus, vitamin C, C6-ceramide and 17?-estradiol) to inhibit smooth muscle cell proliferation at the same time allowing endothelial cells adhesion and proliferation in order to reduce risk associated with vascular implants (Wang, 2009, 2011; Kanie, 2012; Deshpande, 2013; Kakade, 2013; Lamichane, 2013);
Additionally, HAOEC, along with human subclavian artery (HScAEC), carotid artery (HCtAEC), coronary artery (HCAEC) and brachiocephalic artery (HBcAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.Cell Line OriginAortaApplicationcardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testingComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HAOEC Culture Protocol.ПараметрыQuality Level 100 biological source human aorta packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Human Aortic Endothelial Cells: HAOEC: Pre-Screened for Angiogenesis & VEGF signaling, adult S304-05A
NACRES: NA.81 General descriptionEndothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development.
Matched smooth muscle cells obtained from the same arteries are available as well.The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.
VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.
Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells express the following important biomarkers:- Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes;
- eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
- Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
- Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.
Cell Line OriginBlood VesselsApplicationcardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testingComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HAOEC Culture Protocol.ПараметрыQuality Level 100 biological source human blood vessels packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Human Aortic Smooth Muscle Cells: HAOSMC, adult 354-05A
NACRES: NA.81 General descriptionHuman Aortic Smooth Muscle Cells (HAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
HAOSMC have been utilized in dozens of research studies, for example, to:elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Liu, 2009; Tan, 2009; Seymour, 2010; Chen, 2011; Hirase, 2013; Stein, 2013):- Understand hyperglycemia-related risk factors for atherosclerosis in diabetes patients (Maier, 2010), as well as effects of ethanol on vascular calcification (Oros, 2012)
- Investigate extracellular elastic matrix deposition and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Gacchina, 2010, 2011; Venkataraman, 2012; Phillippi, 2013)
- Study mechanisms and effects of mechanoregulation on proliferation and function of smooth muscle cells (Jiang, 2009; Venkataraman, 2013, 2014), and develop advanced stent technology, including novel surface materials, and drug and gene delivery systems to prevent restenosis and other occlusive vasculopathies (Brito, 2010; Deshpande, 2013; Kakade, 2013; Lamichhane, 2013)
Characterization: Positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginAortaApplicationaortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulationComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HAOSMC Culture Protocol.Параметрыbiological source human aorta (normal, tunica intima and tunica media, fibrous plaque-free) Quality Level 100 packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology smooth muscle application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Aortic Smooth Muscle Cells: HAOSMC, fetal 354-05F
NACRES: NA.81 General descriptionHuman Aortic Smooth Muscle Cells (HAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
HAOSMC have been utilized in dozens of research studies, for example, to:elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Liu, 2009; Tan, 2009; Seymour, 2010; Chen, 2011; Hirase, 2013; Stein, 2013);- understand hyperglycemia-related risk factors for atherosclerosis in diabetes patients (Maier, 2010), as well as effects of ethanol on vascular calcification (Oros, 2012);
- investigate extracellular elastic matrix deposition and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Gacchina, 2010, 2011; Venkataraman, 2012; Phillippi, 2013);
- study mechanisms and effects of mechanoregulation on proliferation and function of smooth muscle cells (Jiang, 2009; Venkataraman, 2013, 2014), and develop advanced stent technology, including novel surface materials, and drug and gene delivery systems to prevent restenosis and other occlusive vasculopathies (Brito, 2010; Deshpande, 2013; Kakade, 2013; Lamichhane, 2013).
Characterization: Positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginAortaApplicationaortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulationComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HAOSMC Culture Protocol.Параметрыbiological source human aorta (normal, tunica intima and tunica media, fibrous plaque-free) Quality Level 100 packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology smooth muscle application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Brachiocephalic Artery Endothelial Cells: HBcAEC, adult 3012-05A
NACRES: NA.81 General descriptionHBcAEC, along with human aortic (HAOEC), carotid artery (HCtAEC), coronary artery (HCAEC) and subclavian artery (HScAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.Cell Line OriginArteryApplicationoxygenated blood supply, endothelial permeability, blood albumin leakage, endothelial integrityComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Subculture RoutinePlease refer to the HBcAEC Culture Protocol.ПараметрыQuality Level 100 biological source human brachiocephalic artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Human Brachiocephalic Artery Smooth Muscle Cells: HBcASMC, adult 3512-05A
NACRES: NA.81 General descriptionHuman Brachiocephalic Artery Smooth Muscle Cells (HBcASMC) provide a useful in vitro system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis. Characterization: positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginArteryApplicationcardiovascluar function, oxygenated blood supply to arm, head, neck, blood pressure, artery flexibilityComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Subculture RoutinePlease refer to the HBcASMC Culture Protocol.ПараметрыQuality Level 100 biological source human brachiocephalic artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology smooth muscle application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Bronchial Epithelial Cells: HBEpC, adult 502-05A
NACRES: NA.81 General descriptionHBEpC provide an excellent model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking.
When grown on inserts and provided with the liquid/air interface, HBEpC can differentiate into a pseudostriated epithelium and serve as a more physiological 3D tissue model for in vitro studies.Cell Line OriginBronchiApplicationepithelial function, tissue repair, cell signaling, lung injury treatment, mechanical and oxidative stress tests, cell differentiation, 3D tissue model, cytokine and growth factor production, apoptosis, cell adhesion molecules, cell permeability, pro-inflammatory signaling, viral fusion mechanisms, gene expression, library screening, mucin secretionComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HBEpC Culture Protocol.ПараметрыQuality Level 100 biological source human bronchi (normal surface epithelium) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology epithelial application(s) cell culture | mammalian: suitable relevant disease(s) asthma; cardiovascular diseases; autoimmune diseases shipped in dry ice storage temp. −196°C
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Human Cardiac Fibroblasts: HCF, adult 306-05A
NACRES: NA.81 General descriptionCardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.
HCF has been utilized in a number of research publications, for example to:- Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by large-conductance Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists (Wang, 2006, 2007); and show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites (Dubey, 2005)
- Show that in response to mechanical stretch, cardiac fibroblasts release TGF-b which causes trombomodulin downregulation, increasing the risk of thromboembolic events (Kapur, 2007) and also induces cardiac fibroblast differentiation into myofibroblasts via increased Smad2 and ERK1/2 phosphorylation, that could be stimulated by endothelin-1 and inhibited by Ac-SDKP (Peng, 2010)
- Demonstrate that activation of G protein-coupled receptor kinase-2 (GRK2) prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype (DSouza, 2011); identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology (Ma, 2011) and investigate the roles of scleraxis (Bagchi, 2012) and AMPKa1 (Noppe, 2014) in scar formation following myocardial infarction
- Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance (Liu, 2011)
HCF were also shown to express delayed rectifier IK, Ito, Ca2+-activated K+ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels (Yue, 2013).Cell Line OriginHeartApplicationhuman heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokinesComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 8 doublings
Subculture RoutinePlease refer to the HCF Culture Protocol.Параметрыbiological source human heart (normal adult) Quality Level 100 packaging pkg of 500,000 cells growth mode adherent karyotype 2n = 46 morphology fibroblast application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Cardiac Fibroblasts: HCF, fetal 306-05F
NACRES: NA.81 General descriptionCardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.
HCF has been utilized in a number of research publications, for example to:- Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by large-conductance Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists (Wang, 2006, 2007); and show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites (Dubey, 2005)
- Show that in response to mechanical stretch, cardiac fibroblasts release TGF- which causes trombomodulin downregulation, increasing the risk of thromboembolic events (Kapur, 2007) and also induces cardiac fibroblast differentiation into myofibroblasts via increased Smad2 and ERK1/2 phosphorylation, that could be stimulated by endothelin-1 and inhibited by Ac-SDKP (Peng, 2010)
- Demonstrate that activation of G protein-coupled receptor kinase-2 (GRK2) prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype (DSouza, 2011); identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology (Ma, 2011) and investigate the roles of scleraxis (Bagchi, 2012) and AMPK1 (Noppe, 2014) in scar formation following myocardial infarction
- Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance (Liu, 2011)
HCF were also shown to express delayed rectifier IK, Ito, Ca2+-activated K+ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels (Yue, 2013).Cell Line OriginHeartApplicationhuman heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokinesComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 8 doublings
Subculture RoutinePlease refer to the HCF Culture Protocol.ПараметрыQuality Level 100 biological source human heart (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology fibroblast application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Carotid Artery Endothelial Cells: HCtAEC, adult 3014-05A
General descriptionHCtAEC has been used to elucidate molecular mechanisms of cerebral aneurism caused by haemodynamic (sheer) stress and inflammation, which were shown to act through PGE2-EP2 and NF-B signaling (Aoki, 2011). Demonstrating a link between inflammation and neovascularization, serum amyloid A, a biomarker of inflammation, increased expression of TNF, F3 factor, NF-B and VEGF leading to activated migration, wound healing and tube formation responses in HCtAEC; these pro-angiogenic activities could be prevented by pretreatment with the multi-angiokinase receptor inhibitor BIBF1120 (Cai, 2013). Additionally, CD40-CD154 signaling between endothelial cells and T cells leads to a stress response in endothelial cells via activation of NF-B and MAPK/SAPK pathways and induces down-regulation of APLN, while at the same time activating viral immune surveillance system involving TLR3, IFIH1, RIG-I, and RNASEL (Pluvinet, 2008).
Also, HCtAEC, along with human aortic (HAOEC), brachiocephalic artery (HBcAEC), coronary artery (HCAEC) and subclavian artery (HScAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.Cell Line OriginArteryApplicationneovascularization, cell migration, wound healing, tube formation, angiogenesis, signal tranduction, monocyte chemotactic factor production, cell adhesionComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HCtAEC Culture Protocol.ПараметрыQuality Level 100 biological source human carotid artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Human Carotid Artery Smooth Muscle Cells: HCtASMC, adult 3514-05A
NACRES: NA.81 General descriptionHuman Carotid Artery Smooth Muscle Cells (HCtASMC) provide a useful in vitro system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.Characterization: positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginArteryApplicationcardiovascluar function, transfer of oxygenated blood to skull, brain, neck, face, effects of drugsComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HCtASMC Culture Protocol.ПараметрыQuality Level 100 biological source human carotid artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 23 application(s) cell culture | mammalian: suitable relevant disease(s) stroke; cardiovascular diseases storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Human Chondrocytes - Osteoarthritis: HC-OA, adult 402OA-05A
NACRES: NA.81 General descriptionHC-OA Human Chondrocytes are derived from human articular cartilage of donors with osteoarthritis (OA). Osteoarthritis is an inflammatory disease characterized by increased degradation of cartilage tissue in the joint due to overproduction of enzymes degrading the extracellular matrix. Despite the initial proliferation and activation of chondrocytes, they are not able to efficiently repair the degrading cartilage. Instead, chondrocytes undergo terminal differentiation and eventually apoptose, leading to mineralization of cartilage in a process resembling bone formation during development. Thus, HC-OA provide a useful model to study changes in chondrocyte biology in response to abnormal environment of the OA joint.
Characterization: Positive for aggrecan after differentiation.
HC-OA have been used to demonstrate that THF-alpha activates transcription of ADAMTS-4, a metalloproteinase implicated in cartilage degradation in OA, via p38-MAPK-dependent mechanism (Xue, 2013).Cell Line OriginCartilageApplicationmodel for studying chondrocyte biology in an osteoarthritis jointComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HC-OA Culture Protocol.Параметрыbiological source human articular cartilage (osteoarthritic) Quality Level 100 packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology chondrocyte application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
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Human Chondrocytes - Rheumatoid Arthritis: HC-RA, adult 402RA-05A
NACRES: NA.81 General descriptionHC-RA Human Chondrocytes are derived from human articular cartilage of donors with rheumatoid arthritis (RA). Rheumatoid arthritis is an inflammatory disease that is characterized by overproliferation of synovial cells and their infiltration into adjacent joint tissues, accompanied by gradual destruction of cartilage and bone erosion. Normally, chondrocytes are responsible for producing and maintaining the extracellular matrix of cartilage, but in RA cartilage, the environment is changed significantly due to inflammation and the invasion of other cells. Therefore, HC-RA can be a useful model to study the effects of those changes on chondrocyte biology. In parallel with normal HC, HC-RA can be used to investigate the differences between normal and pathological joints and evaluate potential treatment strategies.
Characterization: Positive for aggrecan after differentiation.Cell Line OriginCartilageApplicationmodel to study effects of RA on chondrocytes, investigate differences between normal and pathological joints and evaluate potential treatment strategiesComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HC-RA Culture Protocol.Параметрыbiological source human articular cartilage (rheumatoid arthritic) Quality Level 100 packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology chondrocyte application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
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Human Chondrocytes: HC, adult 402-05A
NACRES: NA.81 General descriptionHuman Chondrocytes, HC, are derived from normal human articular cartilage where they produce and maintain the extracellular matrix of cartilage, including type II collagen. Chondrocytes grown in monolayer culture on a solid surface tend to lose their phenotypic markers and became de-differentiated to a fibroblast-like phenotype. This de-differentiation stage can be reversed by culturing them in a semi-solid gel.
Normal Chondrocytes have been adapted as an in vitro model system in multiple studies looking for cellular mechanisms, such as inflammation-related signaling cascades, abnormal proteinase production, chondrocyte apoptosis and differentiation, as well as novel potential treatments for arthritic disease.
Characterization: Positive for aggrecan after differentiation.
Normal Human Chondrocytes (HC) have been used to:- Develop a high throughput assay to screen for genes capable of inducing an OA-like phenotype in chondrocytes in order to identify key pathways implicated in the disease (Daouti, 2005)
- Elucidate the signaling cascade leading to induction of proinflammatory cytokines by chondrocytes in RA joints (Aida, 2006; Wang, 2011a,b) and effects of elevated IL-6 signaling on normal chondrocytes (Namba, 2007)
- Investigate mechanisms and identify inhibitors of increased production of proteinases by chondrocytes stimulated by interleukin-1 (Aida, 2005; Wada, 2006), retinoic acid (Hikichi, 2009), proinflammatory cytokines (Tanigawa, 2011a,b), nitric oxide (Wu, 2007; Yang, 2011) and shear stress (Wang, 2011b, 2012)
- Investigate the causes of metalloproteinases induction in patients with Lyme disease-associated arthritis (Lin, 2001; Behera, 2004, 2005)
- Study the factors affecting chondrocyte apoptosis (Cherng, 2008; Malemud, 2012) and differentiation into osteoclasts (Watanabe, 2009a,b; Honda, 2011)
- Show that hyaluronate (HA) can prevent the aggravated cartilage degradation by blocking the matrix metalloproteinases production from cytokine-activated chondrocytes via MKP-1 induction through CD44 signaling (Hashizume, 2009, 2010)
- Demonstrate cytotoxic effects of anti-human Fas/APO-1/CD95 (Fas) monoclonal antibody ARG098 on RA synoviocytes and infiltrating lymphocytes, but not on normal chondrocytes (Tamburstuen, 2010)
Normal Chondrocytes were also utilized in a functional cluster formation agarose assay that allows chondrocytes to maintain their differentiated state (Quintavalla, 2005) and to investigate integrin-mediated mechanotransduction pathways required for proper chondrocyte function (Whitney, 2012). Additionally, they have used extensively in material studies aimed to improve chondrocyte adhesion to medical implants (Gutwein, 2002; Ellison, 2003; Price, 2004; Savaiano, 2004; Burns, 2009) and scaffolds for cartilage regeneration (Jun, 2002; Kay, 2002; Miller, 2002a,b; Price, 2002, 2003; Rao, 2004; Park, 2005; Khang, 2008). Normal chondrocyte RNA was used as a gold standard control in research on cellular reprogramming into chondrocytes (Ishii, 2012).Cell Line OriginCartilageApplicationproduction and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regenerationComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HC Culture Protocol.ПараметрыQuality Level 100 biological source human articular cartilage packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology chondrocyte application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
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Human Chondrocytes: HC, fetal 402-05F
NACRES: NA.81 General descriptionHuman Chondrocytes, HC, are derived from normal human articular cartilage where they produce and maintain the extracellular matrix of cartilage, including type II collagen. Chondrocytes grown in monolayer culture on a solid surface tend to lose their phenotypic markers and became de-differentiated to a fibroblast-like phenotype. This de-differentiation stage can be reversed by culturing them in a semi-solid gel.
Normal Chondrocytes obtained from Cell Applications, Inc. have been adapted as an in vitro model system in multiple studies looking for cellular mechanisms, such as inflammation-related signaling cascades, abnormal proteinase production, chondrocyte apoptosis and differentiation, as well as novel potential treatments for arthritic disease.
Characterization: Positive for aggrecan after differentiation.
Normal Human Chondrocytes (HC) have been used to:- Develop a high throughput assay to screen for genes capable of inducing an OA-like phenotype in chondrocytes in order to identify key pathways implicated in the disease (Daouti, 2005)
- Elucidate the signaling cascade leading to induction of proinflammatory cytokines by chondrocytes in RA joints (Aida, 2006; Wang, 2011a,b) and effects of elevated IL-6 signaling on normal chondrocytes (Namba, 2007)
- Investigate mechanisms and identify inhibitors of increased production of proteinases by chondrocytes stimulated by interleukin-1 (Aida, 2005; Wada, 2006), retinoic acid (Hikichi, 2009), proinflammatory cytokines (Tanigawa, 2011a,b), nitric oxide (Wu, 2007; Yang, 2011) and shear stress (Wang, 2011b, 2012);
- Investigate the causes of metalloproteinases induction in patients with Lyme disease-associated arthritis (Lin, 2001; Behera, 2004, 2005)
- Study the factors affecting chondrocyte apoptosis (Cherng, 2008; Malemud, 2012) and differentiation into osteoclasts (Watanabe, 2009a,b; Honda, 2011)
- Show that hyaluronate (HA) can prevent the aggravated cartilage degradation by blocking the matrix metalloproteinases production from cytokine-activated chondrocytes via MKP-1 induction through CD44 signaling (Hashizume, 2009, 2010)
- Demonstrate cytotoxic effects of anti-human Fas/APO-1/CD95 (Fas) monoclonal antibody ARG098 on RA synoviocytes and infiltrating lymphocytes, but not on normal chondrocytes (Tamburstuen, 2010)
Normal Chondrocytes were also utilized in a functional cluster formation agarose assay that allows chondrocytes to maintain their differentiated state (Quintavalla, 2005) and to investigate integrin-mediated mechanotransduction pathways required for proper chondrocyte function (Whitney, 2012). Additionally, they have used extensively in material studies aimed to improve chondrocyte adhesion to medical implants (Gutwein, 2002; Ellison, 2003; Price, 2004; Savaiano, 2004; Burns, 2009) and scaffolds for cartilage regeneration (Jun, 2002; Kay, 2002; Miller, 2002a,b; Price, 2002, 2003; Rao, 2004; Park, 2005; Khang, 2008). Normal chondrocyte RNA was used as a gold standard control in research on cellular reprogramming into chondrocytes (Ishii, 2012).Cell Line OriginCartilageApplicationproduction and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regenerationComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HC Culture Protocol.ПараметрыQuality Level 100 biological source human articular cartilage packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology chondrocyte application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
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Human Coronary Artery Endothelial Cells: HCAEC, adult 300-05A
NACRES: NA.81 General descriptionHCAEC provides an excellent model system to study all aspects of cardiovascular function and disease, and they have been utilized in dozens of research publications, for example to:- Understand the mechanism of the anti-inflammatory properties of HDL, and demonstrate for the first time that mature miRNA can control gene expression in a cell where it is neither transcribed nor processed (Tabet, 2014)
- Study mechanisms of angiogenesis, as well as oxidative stress and inflammation related pathways in endothelia (Ji, 2009; Wang, 2011; Quinn, 2011; Hung, 2010; Rajesh, 2010; Riegel, 2011; Lin, 2013; Lloid, 2013Baley-Downs, 2012; Kapur, 2012; Melchior, 2012; Castanares-Zapatero, 2013; Hankins, 2013; Lord, 2013; dela Paz, 2013; Takai, 2013), including gender and race specific differences in patients with peripheral artery disease (Gardner, 2014)
- Elucidate molecular mechanisms of various cardiovascular risk factors, including those associated with diabetes (Vladik, 2011; Kapur, 2011; Dunn, 2013; Leucker, 2013; Liu, 2013, 2014; Morgan, 2014; Torella, 2014)
- Understand the mode of action and cardiovascular protection effects of various natural compounds, vitamins and drug candidates (Candelario, 2013; Ramirez-Sanchez, 2010, 2013; Lee, 2013; Nsimba, 2013; Di Bartolo, 2011; Baotic, 2013; Murphy, 2013; Tan, 2013; Wu, 2012), as well as caloric restriction (Csiszar, 2009, 2013)
- Develop and evaluate scaffolds and hydrogels for cardiac tissue engineering (Singelyn, 2009, 2011; Seif-Naraghi, 2010; Johnson, 2014), and new treatment strategies to prevent stent restenosis (ONeill, 2009; OBrien, 2010; Crowder, 2011, 2012; Eppihimer, 2013; Hiob, 2013)
- Compare effects of BMP-4 on HCAEC and Human Pulmonary Artery Endothelial Cells (HPAEC) and show that only in HCAEC BMP-4 treatment induced ROS, activated NF-kB, ICAM-1 and increased monocyte adhesiveness, explaining why its upregulation leads to atherosclerosis and hypertension in the systemic, but not pulmonary circulation (Csiszar, 2008)
Additionally, HCAEC, along with human aortic (HAOEC), carotid artery (HCtAEC), subclavian artery (HScAEC) and brachiocephalic artery (HBcAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.
Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.Cell Line OriginArteryApplicationcardiovascular function, angiogenesis, vascular biology, regulation of coronary blood flow, cardiac functions, effects of vaso-dilators, coagulation, fibrinolysis, vessel wall tone, cytokine productionComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 15 doublings
Subculture RoutinePlease refer to the HCAEC Culture Protocol,.ПараметрыQuality Level 100, biological source human coronary artery (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Coronary Artery Smooth Muscle Cells: HCASMC, adult 350-05A
NACRES: NA.81 General descriptionHuman Coronary Artery Smooth Muscle Cells (HCASMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
HCASMC has been utilized in a number of research studies, for example, to:- Study signaling pathways regulating smooth muscle differentiation (Zhou, 2010); and chronic inflammation of arterial wall that leads to artherosclerosis (Kiyan, 2014)
- Demonstrate that STAT-1 and STAT-3 regulate VEGF production in smooth muscle cells by having opposing effects on HIF-1 expression (Albasanz-Puig, 2012); study the mechanisms of hypoxia and reoxigenation injuries in by demonstrating increased production of ROS and inflammatory cytokines, and further showing that DHA is not beneficial in this type of injuries (Feng, 2012)
- Investigate the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli (Lange, 2013)
- Suggest the hypermethylation of SOCS3 gene as the connection between TNF- and IGF-1 released in response to mechanical injury during coronary intervention, and the induction of cytokines leading to intimal hyperplasia and restenosis (Dhar, 2013)
- Develop a novel VEGFR/MET-targeted inhibitor with improved antitumor efficacy and decreased toxicity (Fujita, 2013); and investigate novel therapies and drug combinations to achieve optimal target selectivity (Lehar, 2009; Wo-Wong, 2013)
- Develop elastic scaffolds for tissue engineering (Nivison-Smith, 2010, 2012) and novel treatment strategies to prevent stent restenosis by designing new materials (Crowder, 2012), or drug therapies to preferentially inhibit smooth muscle cell growth (ONeill, 2009; Mociornita, 2013)
Characterization: positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginArteryApplicationvascular research, supply of blood to heart muscleComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HCASMC Culture Protocol,.Параметрыbiological source human coronary artery (normal, tunica intima and media) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology smooth muscle application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; stroke; cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Dermal Fibroblasts: HDF, adult 106-05A
NACRES: NA.81 General descriptionDermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)Cell Line OriginSkinApplicationinjury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor responseComponentsFibroblast Basal Medium containing 10% FBS & 10%Preparation Note- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10%
- DMSOCan be cultured at least 16 doublings
Culture MediumFibroblast Growth Medium (116-500), all-in-one ready to use mediaSubculture RoutinePlease refer to the HDF Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin or adult skin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Fibroblast application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Fibroblasts: HDF, fetal 106-05F
NACRES: NA.81 General descriptionDermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)Cell Line OriginSkinApplicationinjury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor responseComponentsFibroblast Basal Medium containing 10% FBS & 10% DMSOPreparation Note- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HDF Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin or adult skin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Fibroblast application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Fibroblasts: HDF, neonatal 106-05N
NACRES: NA.81 General descriptionDermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)Cell Line OriginSkinApplicationinjury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor responseComponentsFibroblast Basal Medium containing 10% FBS & 10% DMSOPreparation Note- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HDF Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin or adult skin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Fibroblast application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC, adult 100-05A
NACRES: NA.81 General descriptionCADMEC/HMVEC provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the microvasculature and capillary systems. HMVEC/CADMEC have been shown to express vWF, CD36 and CD31, are able to uptake DiI-Ac-LDL and are positive for functional activity by cytokine-stimulated leukocyte adherence.
Select HMVEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).Cell Line OriginSkinApplicationEndothelial vessel function, cell signaling, angiogenesisComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the CADMEC/HMVEC Culture Protocol,.Параметрыbiological source human neonatal foreskin or adult skin capillaries (normal) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC, neonatal 100-05N
NACRES: NA.81 General descriptionCADMEC/HMVEC provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the microvasculature and capillary systems. HMVEC/CADMEC have been shown to express vWF, CD36 and CD31, are able to uptake DiI-Ac-LDL and are positive for functional activity by cytokine-stimulated leukocyte adherence.
Select HMVEC lots have been additionally tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx).Cell Line OriginSkinApplicationEndothelial vessel function, cell signaling, angiogenesisComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the CADMEC/HMVEC Culture Protocol,.Параметрыbiological source human neonatal foreskin or adult skin capillaries (normal) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC: Pre-Screened for Angiogenesis & VEGF signaling, adult S100-05A
NACRES: NA.81 General descriptionEndothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development.
The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.
VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.
Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells express the following important biomarkers:- Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes;
- eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
- Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
- Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.
Cell Line OriginBlood VesselsApplicationEndothelial vessel function, cell signaling, angiogenesisComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the CADMEC/HMVEC Culture Protocol,.Параметрыbiological source human blood vessels packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable storage temp. −196°C
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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC: Pre-Screened for Angiogenesis & VEGF signaling, neonatal S100-05N
NACRES: NA.81 General descriptionEndothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development.
The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.
VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.
Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells express the following important biomarkers:- Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes; eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
- Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
- Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.
Cell Line OriginBlood VesselsApplicationEndothelial vessel function, cell signaling, angiogenesisComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the CADMEC/HMVEC Culture Protocol,.ПараметрыQuality Level 100, biological source human blood vessels packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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Human Epidermal Keratinocytes: HEK, adult 102-05A
NACRES: NA.81 General descriptionEpidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skins barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.
HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.
When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.Cell Line OriginSkinApplicationSkin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light responseComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HEK Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin or adult skin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Epidermal application(s) cell culture | mammalian: suitable relevant disease(s) allergies; cancer shipped in dry ice storage temp. −196°C
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Human Epidermal Keratinocytes: HEK, fetal 102-05F
NACRES: NA.81 General descriptionEpidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skins barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.
HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.
When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.Cell Line OriginSkinApplicationSkin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light responseComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HEK Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin or adult skin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Epidermal application(s) cell culture | mammalian: suitable relevant disease(s) allergies; cancer shipped in dry ice storage temp. −196°C
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Human Epidermal Keratinocytes: HEK, neonatal 102-05N
NACRES: NA.81 General descriptionEpidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skins barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.
HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.
When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.Cell Line OriginSkinApplicationSkin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light responseComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HEK Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin or adult skin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Epidermal application(s) cell culture | mammalian: suitable relevant disease(s) allergies; cancer shipped in dry ice storage temp. −196°C
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Human Epidermal Melanocytes: HEM, adult 104-05A
NACRES: NA.81 General descriptionEpidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia.Cell Line OriginSkinApplicationmelanin production, melanocyte proliferation, differentiationComponentsBasal Medium containing 30% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Subculture RoutinePlease refer to the HEM Culture Protocol,.Параметрыbiological source human skin (normal abdominal) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Epidermal application(s) cell culture | mammalian: suitable relevant disease(s) metabolic disorders shipped in dry ice storage temp. −196°C
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Human Epidermal Melanocytes: HEM, neonatal 104-05N
NACRES: NA.81 General descriptionEpidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia.Cell Line OriginSkinApplicationmelanin production, melanocyte proliferation, differentiationComponentsBasal Medium containing 30% FBS & 10% DMSOPreparation Note- 3rd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Subculture RoutinePlease refer to the HEM Culture Protocol,.ПараметрыQuality Level 100, biological source human neonatal foreskin (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Epidermal application(s) cell culture | mammalian: suitable relevant disease(s) metabolic disorders shipped in dry ice storage temp. −196°C
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Human Fibroblast-Like Synoviocytes-Osteoarthritis: HFLS-OA, adult 408OA-05A
NACRES: NA.81 General descriptionHFLS-RA provide an excellent cellular model for studying synoviocyte physiology in relation to development and treatment of rheumatoid arthritis.
HFLS-RA have been used in numerous research studies to:- Study signaling pathways implicated in the development of joint inflammation and rheumatoid arthritis (Abe, 2006; Akasaka, 2005; Bai, 2012; Fan, 2013; Furuhashi, 2008; Hashizume, 2008; Matsuyama, 2010; Tsuji, 1999; Yamaguch, 2012a,b; Yano, 2001)
- Evaluate possible contribution of EBV to nonresolving rheumatoid arthritis inflammation through inducing IL-6 production by synoviocytes (Chiu, 2013)
- Evaluate the anti-inflammatory and antirheumatic activity of various compounds, such as celastrol (Venkatesha, 2011; Xu, 2013), triptolide (Kong, 2013; Lin, 2013), arsenic trioxide (Mei, 2011), Wen Luo Yin (Liu, 2013), bucillamine (Tsuji, 2007), therapeutic lyposomes (Harigai, 2007; Ichihara, 2011; Matsumoto, 2013; Negishi, 2011), anti-IL-6R and anti-CD319 antibodies (Suzuki, 2010; Woo, 2013), as well as inhibitors of NF-B (Baima, 2010; Keith, 2005), methionine aminopeptidase-2 (Bernier, 2004), Tpl2 (Hall, 2007), aryl hydrocarbon receptor (Lahoti, 2013) and p38 MAP kinase (Wada, 2005); and additionally demonstrate the beneficial anti-inflammatory effects of low level light therapy (Yamaura, 2009)
- Investigate the causes of metalloproteinases induction in patients with Lyme disease-associated arthritis (Lin, 2001)
- Develop adenovirus-based siRNA delivery system (Arts, 2003)
Additionally, together with normal HFLS, HFLS-RA were used to:- Identify causing agents (such as uric acid crystals or platelet microparticles) and study the immunopathological mechanisms and signal transduction pathways leading to joint inflammation in rheumatoid arthritis (Chen, 2011a; Hsu, 2012; Mathieu, 2008; Tsuji, 2012), and to demonstrate the role of estrogen signaling in increasing inflammation (Galal, 2008)
- Investigate anti-inflammatory properties of herbal compound Sinomenine suggested for rheumatoid arthritis treatment (Chen, 2011b)
- Study the effects of extracellular matrix composition on cell attachment and migration relevant to T-cell function in inflamed tissues (Evanko, 2012)
In parallel with HFLS isolated from joints of patients with osteoarthritis HFLS-RA were used in a study demonstrating the involvement of capsid proteins of parvorvirus B19 in activating synoviocyte migration and induction of the inflammatory response leading to acute symmetrical polyarthropathy (Lu, 2006).
Finally, all three types of HFLS (normal, RA and OA) were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms (Freimanis, 2010).Cell Line OriginBoneApplicationsynoviocyte physiology in relation to development and treatment of rheumatoid arthritis, phenotype, proinflammatory mediator production, signaling pathways, interleukin production, evaluation of anti-inflammatory and antirheumatic compounds, erosive enzyme production (matrix metalloproteinases), cell proliferation and attractionComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 5 doublings
Subculture RoutinePlease refer to the HFLS-OA Culture Protocol,.Параметрыbiological source human synovial tissues (osteoarthritic) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Fibroblast-like application(s) cell culture | mammalian: suitable relevant disease(s) arthritis; coagulation disorders shipped in dry ice storage temp. −196°C
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Human Fibroblast-Like Synoviocytes: HFLS, adult 408-05A
NACRES: NA.81 General descriptionNormal HFLS provide an excellent cellular model for studying the normal and pathological physiology of synoviocytes and development of joint diseases.
Normal HFLS have been used in numerous research studies to:- Study changes in gene expression in synoviocytes stimulated with TNF (Suggiyama, 2002)
- Demonstrate that TNF-like weak inducer of apoptosis (TWEAK) contributes to joint inflammation by inducing chemokines such as MIP-1 (CCL-4), lymphotactin (XCL-1), IFN--inducible protein 10 (IP-10) (CXCL-10), MCP-1 (CCL-2), and RANTES (CCL-5), as well as the matrix metalloprotease-9, suggesting TWEAK as a new therapeutic target (Perper, 2006)
- Demonstrate the role of miR-124a in arthritis pathogenesis (Nakamachi, 2009)
- Show that C/EBP regulates expression of metalloproteinases and ADAMTS family members in synoviocytes stimulated with IL-1 (Tsushima, 2012); and that HMW-HA suppressed ADAMTS4 mRNA and protein expression via CD44, p38 MAPK and JNK pathways (Kataoka, 2013)
- Evaluate the anti-inflammatory and antirheumatic activity of various compounds, such as NF-B inhibitors (Wen, 2006), AGIX-4207 (Kunsch, 2005), HA–methotrexate conjugates (Homma, 2009) and bucillamine (Oki, 2009)
- Show that reactive arthritis triggered by chlamydial infection is mediated by TLR2 induced IL-6 production in synoviocytes (Konomi, 2009); and investigate the mechanisms of arthritis-like syndrome in patients infected with chikungunya (CHIK) virus (Xu, 2013)
- Suggest that only leukocyte-poor, RBC-free platelet-rich plasma should be used in clinical orthopaedics because leukocytes and RBCs cause synoviocyte death and proinflammatory mediator production (Brown, 2014)
- Identify that cartilage link protein and MAGP2 can be used as specific markers to distinguish chondrocytes and synovial cells (Rapko, 2007, 2010)
- Create, along with human dermal fibroblasts, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail”, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takahashi, 2007)
Additionally, in parallel with HFLS isolated from joints of patients with rheumatoid arthritis, normal HFLS were used to:- Identify causing agents (such as uric acid crystals or platelet microparticles) and study the immunopathological mechanisms and signal transduction pathways leading to joint inflammation in rheumatoid arthritis (Chen, 2011a; Hsu, 2012; Mathieu, 2008; Tsuji, 2012), and to demonstrate the role of estrogen signaling in increasing inflammation (Galal, 2008)
- Investigate anti-inflammatory properties of herbal compound Sinomenine suggested for rheumatoid arthritis treatment (Chen, 2011b)
- Study the effects of extracellular matrix composition on cell attachment and migration relevant to T-cell function in inflamed tissues (Evanko, 2012)
Finally, all three types of HFLS (normal, RA and OA) were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms (Freimanis, 2010).Cell Line OriginBoneApplicationsynoviocyte physiology, joint homeostasis, production of collagen, glycoproteins, lubrican, vimentin and hyaluronic acid, gene expression, signal transduction pathways, enzyme production, evaluation of anti-inflammatory agents, effects of extracellular matrix, cell attachment, migration, integrin study, proinflammatory mediator productionComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 5 doublings
Subculture RoutinePlease refer to the HFLS Culture Protocol,.Параметрыbiological source human synovial tissues (normal) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Fibroblast-like application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
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Human Internal Thoracic Artery Endothelial Cells: HITAEC, adult 308-05A
NACRES: NA.81 General descriptionHITAEC has been used to investigate oral pathogens travel through the body by infecting dendritic cells and inducing them to migrate through endothelial layers by causing upregulation of CXCR4 (Miles, 2014). To identify genes that make Human Internal Thoracic Arteries resistant to atherosclerosis, HITAEC and HCAEC (derived from the athero-prone human coronary arteries) were subjected to microarray analysis (Archaki, 2012). Twenty-nine genes were found to be expressed differently between HITAEC and HCAEC; one of them (TES) was shown to be downregulated in patients with coronary artery disease, resulting in increased monocyte adhesion, endothelial cell motility and transendothelial migration of monocytes.Cell Line OriginArteryApplicationcoronary artery bypass grafting, myocardial revascularization, endothelial cell motility, transendothelial migration, gene expression, contractile responses, receptor stimulation, cell relaxation, supply of oxygenated blood to chest wall and breasts,ComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HITAEC Culture Protocol,.Параметрыbiological source human intrathoratic artery (normal) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Internal Thoracic Artery Smooth Muscle Cells: HITASMC, adult 358-05A
NACRES: NA.81 General descriptionHuman Internal Thoracic Smooth Muscle Cells (HITASMC) are derived from the normal internal thoracic arteries (formerly called internal mammary arteries).HITASMC, along with Human Coronary Artery Smooth Muscle Cells, both from Cell Applications, Inc. have been used to investigate the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli (Lange, 2013).Characterization: positive for smooth muscle cell specific alpha-actin expression.Cell Line OriginArteryApplicationgene expression, response to injury and proliferation stimuli, blood supply to anterior chest wall and breasts, coronary artery bypass grafting, revascularizationComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 8 doublings
Subculture RoutinePlease refer to the HITASMC Culture Protocol,.ПараметрыQuality Level 100, biological source human intrathoratic artery packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology smooth muscle application(s) cell culture | mammalian: suitable relevant disease(s) diabetes; cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Lung Fibroblasts: HLF, adult 506-05A
NACRES: NA.81 General descriptionHLF provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.
HLF have been utilized in a number of research publications, for example to:- Demonstrate differential gene expression patterns between fetal and adult lung fibroblasts, in particular showing that fetal fibroblasts express higher level of TGF- receptor interacting protein 1 (TRIP-1) and consequently are less able to contract collagen than adult lung fibroblasts, leading to better healing of acute injuries in very immature lungs compared to higher levels of fibrosis observed in older patients (Navarro, 2009), and additionally discover that TRIP-1 regulates lung fibroblast/myofibroblast trans-differentiation (Nyp, 2014)
- Show that angiotensin II promotes lung fibrosis by activating expression of IL-4, IL-7, and PDGF-D increasing the extracellular matrix formation, and that this process can be blocked by angiotensin receptor inhibitor candesartan (Okada, 2009)
- Determine that phosphorylation of C/EBP contributes to lung fibrosis (Buck, 2011)
- Demonstrate that increased tissue rigidity activates toll-like receptor 2 and NF-B in lung fibroblasts and induces expression of cytokines, CXCL1-3, IL-8 and TNF- (Zheng, 2011)
- Demonstrate that 2-ethoxyestradiol, a non-estrogenic estradiol metabolite analog, inhibits cell growth and retards the progression of pulmonary fibrosis and hypertension (Tofovic, 2008, 2009)
- Identify the hypoxia-induced ROS/RhoA–ROCK dependent actin reorganization as a mediator of myofibroblast transformation and collagen synthesis in lung fibrosis (Ni, 2013)
Cell Line OriginLungsApplicationpulmonary function, gene expression, healing and repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical developmentComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Subculture RoutinePlease refer to the HLF Culture Protocol,.Параметрыbiological source human lung (normal parenchyma) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology fibroblast application(s) cell culture | mammalian: suitable relevant disease(s) asthma; tuberculosis; cardiovascular diseases; cancer shipped in dry ice storage temp. −196°C
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Human Lung Fibroblasts: HLF, fetal 506-05F
NACRES: NA.81 General descriptionHLF provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.
HLF have been utilized in a number of research publications, for example to:- Demonstrate differential gene expression patterns between fetal and adult lung fibroblasts, in particular showing that fetal fibroblasts express higher level of TGF- receptor interacting protein 1 (TRIP-1) and consequently are less able to contract collagen than adult lung fibroblasts, leading to better healing of acute injuries in very immature lungs compared to higher levels of fibrosis observed in older patients (Navarro, 2009), and additionally discover that TRIP-1 regulates lung fibroblast/myofibroblast trans-differentiation (Nyp, 2014)
- Show that angiotensin II promotes lung fibrosis by activating expression of IL-4, IL-7, and PDGF-D increasing the extracellular matrix formation, and that this process can be blocked by angiotensin receptor inhibitor candesartan (Okada, 2009)
- Determine that phosphorylation of C/EBP contributes to lung fibrosis (Buck, 2011)
- Demonstrate that increased tissue rigidity activates toll-like receptor 2 and NF-B in lung fibroblasts and induces expression of cytokines, CXCL1-3, IL-8 and TNF- (Zheng, 2011)
- Demonstrate that 2-ethoxyestradiol, a non-estrogenic estradiol metabolite analog, inhibits cell growth and retards the progression of pulmonary fibrosis and hypertension (Tofovic, 2008, 2009)
- Identify the hypoxia-induced ROS/RhoA–ROCK dependent actin reorganization as a mediator of myofibroblast transformation and collagen synthesis in lung fibrosis (Ni, 2013)
Cell Line OriginLungsApplicationpulmonary function, gene expression, healing and repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical developmentComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 12 doublings
Subculture RoutinePlease refer to the HLF Culture Protocol,.Параметрыbiological source human lung (normal parenchyma) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology fibroblast application(s) cell culture | mammalian: suitable relevant disease(s) asthma; tuberculosis; cancer; cardiovascular diseases shipped in dry ice storage temp. −196°C
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Human Lung Microvascular Endothelial Cells: HLMVEC, adult 540-05A
NACRES: NA.81 General descriptionThe pulmonary vasculature is of great physiological/pathological significance. It is now recognized to be a tissue composed of metabolically active, functionally responsive cells, that interact with circulating substrates and regulate the composition of systemic arterial blood, affect target organ functions, and contribute to thrombosis, hemostasis and immune reactions, as well as tumor metastasis. Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro.
HLMVEC have been used to elucidate the therapeutic effects of Angiotensin I-converting enzyme (ACE) inhibitors, and the results revealed that they provide an additional benefit to patients by activating bradykinin B1 receptor leading to prolonged nitric oxide (NO) production in endothelial cells and inhibition of PKC (Ignjatovic, 2004; Stanisavljevic, 2006).Cell Line OriginLungsApplicationlung physiology, metabolism, regulate blood composition, hemostasis, immune reactions, therapeutic effects, leukocyte migration,ComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 3rd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 15 doublings
Subculture RoutinePlease refer to the HLMVEC Culture Protocol,.Параметрыbiological source human lung (normal) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology Endothelial application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
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Human Mammary Epithelial Cells: HMEpC, adult 830-05A
NACRES: NA.81 General descriptionMammary Epithelial Cells provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to cancerogenesis.
HMEpC have been utilized in numerous research publications, for example to:- Investigate the role of exosomes secreted by cancer cells in formation of tumor permissive microenvironment through manipulation of normal mammary epithelium (Dutta, 2014)
- Serve as control in a study investigating antitumor properties of cannabinoids (Ligresti, 2006) and stem cell microenvironment (Rostovit, 2008)
- Determine that differential expression of glycoproteins allows to classify human breast cells into normal, benign, malignant, basal, and luminal groups (Yen, 2011, 2013; Timpe, 2013)
- Identify ALDH isoform 5A1 as a potential target for treatment of human breast ductal carcinoma (Kaur, 2012); and determine that combination of an anti-EGFR anti-VEGFR treatment using ZD6474 with phototherapy (UV-B) is more effective in treating breast cancer than either treatment alone (Sarkar, 2013)
- Demonstrate that although SIRT deacetylates p53, it does not play a role in cell survival following DNA damage (Solomon, 2006)
- Demonstrate the important roles of tumor suppressor Maspin by showing that it promotes mammary epithelial differentiation via its interaction with IRF6 (Bailey, 2008); mediates effects of IFN- on vacuolar pH, cathepsin D processing and autophagy, protects extracellular matrix (ECM) from degradation in normal mammary epithelia, and that Maspin loss in metastatic cancer leads to unrestricted ECM degradation, contributing to metastasis (Khalkhali-Ellis, 2007, 2008); and show that loss of EcSOD expression also promotes invasiveness by disrupting ECM (Teoh-Fitzgerald, 2012, 2013)
- Investigate the role of shortened telomeres in initiation of genomic instability, cytokinesis failure and polyploidy (Tusell, 2008; Soler, 2009; Pampalona, 2012); and elucidate the role of Myc in malignancy by studying its ability to transform primary epithelial cells (Thibodeaux, 2009)
- Demonstrate, along with Human dermal Fibroblasts, that resveratrol inhibits mono-ubiquitination of histone H2B (Gao, 2011)
HMEpC, along with Human Prostate Epithelial Cells, have also been used in a study demonstrating that TGF-b1 induces Smad 1/5/8 and Smad 2/3 phosphorylation and BMP signaling results only in Smad 1/5/8 phosphorylation in these primary epithelial cells, while in cancer cells BMP also elicits Smad2/3 activation, contributing to cancerogenesis (Holtzhausen, 2013).
Characterization: the cells have a characteristic morphology consistent with an epithelial origin and are positive for epithelial cell marker cytokeratin 18.Cell Line OriginMammary GlandApplicationantitumor properties, glycoprotein expression, breast cancer treatment approaches, DNA damage, cell differentiation, effects of growth factorsComponentsCell Basal Medium containing 10% FBS & 10% DMSOPreparation Note- 5th passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Subculture RoutinePlease refer to the HMEpC Culture Protocol,.Параметрыbiological source human mammary glands (normal) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology epithelial application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in liquid nitrogen storage temp. −196°C
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Human Marrow Stromal Cells: HMSC, adult 492-05A
NACRES: NA.81 General descriptionBone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, marrow stromal cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages. The potential of HMSCs to maintain multipotency and proliferate extensively in vitro provides new avenues for cell-based therapy in the restoration of damaged or diseased tissue.
HMSC have been utilized to investigate the effects of TGF-1 on differentiation toward different lineages, and show that it inhibits adipogenic differentiation and induces either chondrogenic, osteogenic or smooth muscle cell differentiation, depending the specific culture conditions (Zhao, 2011); to develop methods to assess differentiation potential of stem cells without the differentiation induction (Hirota, 2011); to compare HLA expression profiles of bone marrow-derived and adipose-derived stem cells (Purandare, 2013); and to design heart valve-shaped tri-layered collagen-based constructs for stem cell seeding and differentiation to use in heart valve tissue engineering (Tedder, 2010, 2011).Cell Line OriginBone MarrowApplicationblood production, hematopoietic growth and differentiation, pathways of pluripotent cell differentiation into bone, cartilage and fat, connective tissue production,supportive structure, cell production, cell proliferation, cell based therapy, tissue restoration and repair, differentiation, effects of culture conditinos, HLA expression, cytokine production, immunomodulation, clinical trialsComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HMSC Culture Protocol,.ПараметрыQuality Level 100, biological source human bone marrow packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology stromal application(s) cell culture | mammalian: suitable relevant disease(s) tuberculosis shipped in dry ice storage temp. −196°C
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Human Marrow Stromal Cells: HMSC, fetal 492-05F
NACRES: NA.81 General descriptionBone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, marrow stromal cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages. The potential of HMSCs to maintain multipotency and proliferate extensively in vitro provides new avenues for cell-based therapy in the restoration of damaged or diseased tissue.
HMSC have been utilized to investigate the effects of TGF-1 on differentiation toward different lineages, and show that it inhibits adipogenic differentiation and induces either chondrogenic, osteogenic or smooth muscle cell differentiation, depending the specific culture conditions (Zhao, 2011); to develop methods to assess differentiation potential of stem cells without the differentiation induction (Hirota, 2011); to compare HLA expression profiles of bone marrow-derived and adipose-derived stem cells (Purandare, 2013); and to design heart valve-shaped tri-layered collagen-based constructs for stem cell seeding and differentiation to use in heart valve tissue engineering (Tedder, 2010, 2011).Cell Line OriginBone MarrowApplicationblood production, hematopoietic growth and differentiation, pathways of pluripotent cell differentiation into bone, cartilage and fat, connective tissue production,supportive structure, cell production, cell proliferation, cell based therapy, tissue restoration and repair, differentiation, effects of culture conditinos, HLA expression, cytokine production, immunomodulation, clinical trialsComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HMSC Culture Protocol,.ПараметрыQuality Level 100, biological source human bone marrow packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology stromal application(s) cell culture | mammalian: suitable relevant disease(s) tuberculosis shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Human Mononuclear Cells: HMNC, adult 690PB-100A
NACRES: NA.81 General descriptionThe leukocytes can be divided into two groups: mononuclear and granulocytic cells. The mononuclear cells mainly consist of lymphocytes and monocytes. The lymphocyte population consists of T cells (CD4 and CD8 positive ~75%), B cells and NK cells (~25% combined). In contrast to the granulocytes, these cells have rounded nuclei, some with indentations or folds. The mononuclear cells are involved in various ways with the bodys defense mechanisms.
1. These cells are critical components in the immune system to fight bacterial, viral infections, and function as immune surveillance network to kill tumor cells. The mononuclear cells also secrete a great variety of cytokines to regulate immune response as well as inflammation. Moreover, it has been demonstrated that the mononuclear cells may mediate various biological effects on other tissue and organs. For example, the chemokines produced by monocytes can act on adipocytes and muscles. Human Mononuclear Cells (HMNC) are often extracted from from bone marrow (BM) or peripheral blood (PB) using Ficoll-Hypaque density gradient centrifugation, a hydrophilic polysaccharide that separates layers of blood, with mononuclear cells forming a buffy coat under a layer of plasma. Generally, this buffy coat mainly contains lymphocytes and monocytes, other cell types such as cells are detected in Human Mononuclear Cell (HMNC) preparation.
2. Human Mononuclear Cells (HMNC) are widely used in research and clinical applications every day. The HMNCs are important tool for various studies in the fields of immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, transplant immunology, and high-throughput screening.
3. HIV research uses them because PBMCs include CD4+ cells, which are the cells HIV infects.
4. Moreover, HMNCs have been accepted as a unique material for cancer immunotheurapy using dendritic cells or activated lymphocytes as an alternative or adjuvant to conventional therapies such as surgery, chemotherapy and radiation treatment.
5,6. Endothelial progenitor cells derived from HMNC have been used in neovascularization to improve limb ischemia
7. In cell therapy to repair ischemic and infracted myocardium.
8. Human Mononuclear Cells (HMNC) should provide a useful tool for studying various aspects of pathology and biology of the human mononuclear cells in vitro.
Human Mononuclear Cells (HMNC) are derived from human peripheral blood (HMNC-PB) using Ficoll-Hypaque density gradient centrifugation. They are cryopreserved immediately after isolation.
1. Allison, A.C. et al, Annu. Rev. Immunol. 1:361 (1983); 2. Sales, V.L. et al, Tissue Eng. 13:525 (2007); 3. Juranic, Z. et al, J. Exp. Clin. Cancer Res. 18:317 (1999); 4. Vicenzi E. et al, J. Leukoc. Biol. 68:405 (2000); 5. Steinman, R. M. et al, Int. J. Cancer 94:459 (2001); 6. Schuler, G., Cancer Immunity 3 (Suppl 1):23 (2003); 7. Kalka, C. et al, Proc. Natl. Acad. Sci. USA 97:3422 (2000); 8. Masuda, H. et al, Cardiovasc. Res. 58:390 (2003)Cell Line OriginHuman Peripheral BloodApplicationimmune surveillance and defense, cytokine and chemokine production, immunoregulation, research, clinical applications, vaccine development, transplant immunology, high-throughput screening, HIV research, cancer immunotherapy, infection fighting, study of T, B and NK cells,ComponentsHMNC-PB in DMEM containing 45% FBS & 10% DMSOPreparation Note- Immediately after isolation, 10,000,000 HMNC-PB in DMEM containing 45% FBS & 10% DMSO
- Blood cells do not proliferate in culture
Subculture RoutinePlease refer to the HMNC-PB Culture Protocol,.Параметрыbiological source human peripheral blood packaging pkg of 10,000,000 cells (HMNC-PB) growth mode Suspension karyotype 2n = 46 morphology mononuclear application(s) cell culture | mammalian: suitable relevant disease(s) acquired immunodeficiency syndrome/human immunodeficiency virus (AIDS/HIV); infectious diseases; autoimmune diseases shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Human Osteoblasts-Osteoarthritis: HOb-OA, adult 406OA-05A
NACRES: NA.81 General descriptionHuman Osteoblasts-Osteoarthritis, HOb-OA, are isolated from bone of patients with osteoarthritis (OA) and provide an excellent model system to study the patophisiology of that disease in vitro, including matrix mineralization, effects of inflammatory mediators and bone morphogenic proteins, physiological control of bone remodeling and regulation of bone metabolism.
Characterization: Positive for bone mineralization.Cell Line OriginBoneApplicationmodel system to study the pathophisiology of osteoarthritis, matrix mineralization, effects of inflammatory mediators, bone morphogenic proteins, bone remodeling and metabolismComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HOb-OA Culture Protocol,.Параметрыbiological source human bone (osteoarthritic) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology osteoblast application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Human Osteoblasts-Rheumatoid Arthritis: HOb-RA, adult 406RA-05A
General descriptionHuman Osteoblasts-Rheumatoid Arthritis, HOb-RA, are isolated from bone of patients with rheumatoid arthritis (RA) and provide an excellent model system to study the patophisiology of that disease in vitro, including matrix mineralization, effects of inflammatory mediators and bone morphogenic proteins, physiological control of bone remodeling and regulation of bone metabolism.Characterization: Positive for bone mineralization.Cell Line OriginBoneApplicationmodel system to study the pathophisiology of rheumatoid arthritis, matrix mineralization, effects of inflammatory mediators, bone morphogenic proteins, bone remodeling and metabolismComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HOb-RA Culture Protocol,.Параметрыbiological source human bone (rheumatoid arthritic) Quality Level 100, packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology osteoblast application(s) cell culture | mammalian: suitable relevant disease(s) arthritis shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Human Osteoblasts: HOb, adult 406-05A
NACRES: NA.81 General descriptionHOb are isolated from fetal or adult human bones. Additionally, osteoblasts from osteoarthritis or rheumatoid arthritis donors are available. Human osteoblasts provide an excellent model system for studies relevant to the skeletal system.
Characterization: Positive for bone mineralization.
Human osteoblasts have been used to:- Characterize signaling pathways mediating bone morphogenesis, such as identify human Crossveinless-2 as an inhibitor of BMP (Binnerts, 2009); demonstrate crosstalk between Runx2, Osterix, and NELL-1 (Chen, 2011); identify TWEAK as a cytokine regulating RANTES production, BMP-2-induced differentiation, and RANKL expression in osteoblasts (Ando, 2006)
- Demonstrate that Kobophenol A enhances proliferation of osteoblasts via activation of the p38 pathway (Kwak, 2013) and clarify the relationship between hypertension and osteoporosis by demonstrating that Ang II activates osteoclasts via RANKL induction (Shimizu, 2008, 2012)
- Identify adiponectin as an activator of IL-6, IL-8, VEGF and MMPs in endothelial cells and osteoblasts, implicating it in the development of arthritis (Lee, 2014)
- Show that inhibition of NF-B prevented the progression of bone loss in periodontitis and promoted the wound healing in bone defects through the inhibition of osteoclasts (Shimizu, 2009)
- Study active compounds from Uraria crinita for their potential to stimulate bone formation and regeneration (Mao, 2014)
- Investigate properties of osteosarcoma, including the role of fluid pressure in angiogenesis (Nathan, 2008) and novel oncogenes (Both, 2012); and develop better treatment strategies (Ma, 2011)
- Develop optimal materials, coatings and drug delivery techniques for orthopedic implants and tissue engineering (Frandsen, 2014; Ni, 2012; Pilia, 2013a, b, 2014; Shiels, 2012; Valente, 2012; Zhang, 2010, 2011)
Cell Line OriginBoneApplicationmodel for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatmentsComponentsBasal Medium containing 10% FBS & 10% DMSOPreparation Note- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture RoutinePlease refer to the HOb Culture Protocol,.Параметрыbiological source human bone (normal) packaging pkg of 500,000 cells growth mode Adherent karyotype 2n = 46 morphology osteoblast application(s) cell culture | mammalian: suitable relevant disease(s) arthritis; osteoporosis shipped in dry ice storage temp. −196°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену
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