Nuclei Isolation Kit: Nuclei PURE Prep NUC201-1KT

NACRES:

NA.32

For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.
The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

usage	sufficient for 15 nuclei preparations (~1-10x107 cells or 1g of tissue per preparation)
Quality Level	200
packaging	pkg of 1 kit
storage condition	dry at room temperature
application(s)	cell analysis: suitable
foreign activity	nuclease and protease, free
shipped in	wet ice
storage temp.	2-8°C

Pictograms	GHS05
Signal Word	Danger
Hazard Statements	H318 - H412
Precautionary Statements	P273 - P280 - P305 + P351 + P338 + P310
RIDADR	NONH for all modes of transport
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable