Nucleases Sigma-Aldrich
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Deoxyribonuclease I bovine
Enzyme Commission (EC) Number: 3.1.21.1 ( BRENDA | IUBMB ) MDL number: MFCD00130918 NACRES: NA.54
Кат. номер D5319-2MG-PW D5319-500UG D5319-2MG D5319-500UG-PW ApplicationDeoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.
DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study. The enzyme has also been for the preparation and harvest of mice mammary glands.
Used for the removal of DNA from protein samples.
Biochem/physiol Actions
DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5 phosphates and 3 OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.
Features and Benefits- RNA purification by removing DNA
- Prepare DNA for nick translation1
- Footprinting assays to determine DNA-protein interactions2
Unit Definition
One unit will produce a A260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C
Physical form
Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol
Preparation Note
Produced without using any animal cells or animal derived materials.Параметрыrecombinant expressed in Pichia pastoris Quality Level 200, form buffered aqueous glycerol solution specific activity 5,000 units/mg protein mol wt ~39 kDa foreign activity RNAse and protease, free shipped in dry ice storage temp. 20°C
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Deoxyribonuclease I from bovine pancreas
CAS Number: 9003-98-9 Enzyme Commission (EC) Number: 3.1.21.1 ( BRENDA | IUBMB ) EC Number: 232-667-0 MDL number: MFCD00130918
Кат. номер DN25-100MG DN25-10MG DN25-PM DN25-5G DN25-1G General descriptionDNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.- Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
- Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
- DNase I structure resembles the structure of to exonuclease III. It includes two central sheets. Each sheet is composed of six -strands. This complex of sheets is surrounded by extensive loop and -helical regions. This enzyme shares structural similarity to exonuclease III.
Application- Decreases viscosity providing better yields by removing DNA in primary cell isolation:
- Incorporating labelled bases into DNA: DNA nick
- Radioactive labelling
- Bioprocessing applications: DNA removal
- Eliminating genomic DNA from RNA preparations before RT-PCR
- In vitro transcription
- Nick translation
- DNase footprinting
- Actin regulation of actin polymerization in cells, and cell apoptosis
- UV crosslinking of proteins to nucleic acids
- DNase play a role in the regulation of actin polymerization in cells and is involved in apoptosis process
Used for the removal of DNA from protein samples.Packaging10, 100 mg in poly bottle
1, 5 g in poly bottleBiochem/physiol ActionsDNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.Features and BenefitsOur Deoxyribonuclease DNAse I,is essentially RNAse free product, which support the product application.Unit DefinitionOne Kunitz unit will produce a change in A260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III, as substrate.Physical formCrude preparation, contains calcium chloridePreparation Note10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.Analysis NoteProtein determined by biuret.ПараметрыQuality Level 300 form lyophilized powder specific activity 400 Kunitz units/mg protein mol wt ~31 kDa composition Protein, 85% solubility 0.15 M NaCl: soluble 5.0 mg/mL, hazy Featured Industry Diagnostic Assay Manufacturing
Diagnostic Assay Manufacturingforeign activity RNase 0.02% shipped in wet ice storage temp. 20°C
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Deoxyribonuclease II from bovine spleen
CAS Number: 9025-64-3 Enzyme Commission (EC) Number: 3.1.22.1 ( BRENDA | IUBMB ) EC Number: 232-801-8 MDL number: MFCD00130919 NACRES: NA.54
Кат. номер D8764-30KU D8764-300KU D8764-150KU ApplicationDeoxyribonuclease II from bovine spleen has been used:- as a component of digestion mix to isolate immune cells from colon samples
- to digest retinal explants for single-cell suspension preparation
- for isolation of stromal cells from human colon tissue
- in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart
Packaging
30000, 150000, 300000 units in poly bottle
Biochem/physiol Actions
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.
Unit Definition
One Kunitz unit will produce a A260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mMПараметрыtype Type V Quality Level 200, form essentially salt-free, lyophilized powder specific activity ≥1,000 units/mg protein mol wt 38 kDa foreign activity RNase ≤0.1% storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Nuclease micrococcal from Staphylococcus aureus
CAS Number: 9013-53-0 Enzyme Commission (EC) Number: 3.1.31.1 ( BRENDA | IUBMB ) EC Number: 232-748-0 MDL number: MFCD00131731 NACRES: NA.54
Кат. номер N3755-200UN N3755-500UN N3755-50UN-PW N3755-200UN-PW N3755-500UN-PW N3755-50UN ApplicationNuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.Packaging50, 200, 500 units in serum bottleBiochem/physiol ActionsCleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.
Hydrolyzes 5′-phosphodiester bonds of DNA.Unit DefinitionOne unit will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.Other NotesNote: One µmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a A260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.ПараметрыQuality Level 300 form lyophilized powder specific activity 100-300 units/mg protein composition Protein, 40% E1%/280 (Balance primarily sodium citrate) Featured Industry Diagnostic Assay Manufacturing storage temp. 20°C Gene Information Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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Nuclease S1 from Aspergillus oryzae N5661-50KU
CAS Number: 37288-25-8 Enzyme Commission (EC) Number: 3.1.30.1 ( BRENDA | IUBMB ) MDL number: MFCD00131010 NACRES: NA.54 General descriptionThe Nuclease S1 enzyme from Aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides.ApplicationNuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. It has also been used in a study to investigate the DNA damage and repair in a -irradiated rat brain tumor.Biochem/physiol ActionsSI nuclease from Aspergillus oryzae can generate double-stranded DNA breaks in response to DNA nicks or abasic sites.
Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5-phosphomononucleotide and 5-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.Unit DefinitionOne unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C.Physical formSolution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl2, 50% glycerol, 2 mg/ml proteinПараметрыQuality Level 200 form solution concentration 100000 units/mL shipped in wet ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Ribonuclease A from bovine pancreas
CAS Number: 9001-99-4 Enzyme Commission (EC) Number: 3.1.27.5 ( BRENDA | IUBMB ) EC Number: 232-646-6 MDL number: MFCD00132181 NACRES: NA.54
Кат. номер R5503-1G-C R5503-500MG-C R5503-5G-C R5503-10G-C R5503-5G R5503-10G R5503-1G R5503-500MG R5503-100MG General descriptionRNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.Application- RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
- RNase A is also used in RNA sequence analysis and protection assays.
- RNase A has been used as a tool for computer-aided drug design.
- RNase A supports the analysis of RNA sequences.
- RNase A hydrolyze RNA contained in protein samples.
- Purification of DNA is supported by RNase A.
Packaging100, 500 mg in poly bottle
1, 5, 10 g in poly bottleBiochem/physiol ActionsRibonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3 phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.Features and BenefitsOur highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.Preparation NoteSalt fractionated and chromatographically purified.Analysis NoteProtein determined by E.ПараметрыQuality Level 200 type Type I-AS form lyophilized powder specific activity 50-100 Kunitz units/mg protein mol wt ~13,700 impurities salt, essentially free Featured Industry Diagnostic Assay Manufacturing foreign activity protease, essentially free storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P501 Personal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
RNase B Glycoprotein Standard from bovine pancreas
CAS Number: 9001-99-4 EC Number: 232-646-6 MDL number: MFCD00132184 NACRES: NA.32
Кат. номер R1153-500UG-PW R1153-1MG-KC R1153-PH R1153-1MG-PW R1153-1MG R1153-500UG ApplicationRNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B has been used as a source of N-glycans following enzymatic digestions and subsequent purification. It has also been used as a glycoprotein standard.Other NotesBovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn34 at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man5-9GlcNAc2. Due to the heterogeneity in the glycosylation at Asn34, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa.
Quality
RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A.ПараметрыQuality Level 200, grade Proteomics Grade assay ≥90% (SDS-PAGE) form lyophilized powder storage temp. −20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P501 Personal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Salt Active Nuclease
Кат. номер SRE0015-25KU-PW SRE0015-5KU SRE0015-25KU SRE0015-5KU-PW ApplicationSalt active nuclease has been used to remove DNA during protein expression and purification.Biochem/physiol ActionsSalt active nuclease (SAN) is a general, unspecific endonuclease that cleaves double-stranded and single-stranded DNA, and RNA. SAN is active at above neutral pH. Unlike other nucleases, however, it has optimum activity at high concentrations of salt at high pH. These qualities make SAN ideal for removal of DNA from cell extracts and protein samples. The end-products upon complete degradation of DNA consist of a majority of 5 nucleotide
oligos. The enzyme degrades DNA vs. RNA in a ratio of 10:1.
SAN is highly active over the temperature range 10-40 °C. The optimal NaCl-concentration for activity is 0.5 M. However, SAN is active in low salt buffers. 1 M NaCl is not recommended for use of SAN. Mg2+ (>1 mM) is required for activity. The pH working range is above neutral pH, with an optimal range between pH 8.5-9.5
Physical form: Solution in 25 mM Tris-HCl (pH 7.5), 5 mM MgCl2, 0.5 M NaCl, 0.01% Triton, and 50% (v/v) glycerol
Unit definition: One unit (U) is defined as an increase in absorbance at 260 nm of 1 Absorbance Unit in 30 minutes at 37 C, using 50 µg/ml calf thymus DNA in a buffer with 25 mM Tris-HCl (pH 8.5, 25 C), 5 mM MgCl2, and 500 mM NaCl.Unit DefinitionOne unit (U) is defined as an increase in absorbance at 260 nm of 1 absorbance unit (AU) in 30 minutes at 37 °C, using 50 µg/ml calf thymus DNA in a buffer with 25 mM Tris-HCl (pH 8.5, 25 °C), 5 mM MgCl2, and 500 mM NaCl.ПараметрыQuality Level 200 recombinant expressed in Pichia pastoris form buffered aqueous glycerol solution specific activity 250000 U/mg shipped in wet ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Turbonuclease from Serratia marcescens
CAS Number: 9025-65-4 Enzyme Commission (EC) Number: 3.1.30.2 ( BRENDA | IUBMB ) MDL number: MFCD00131010
Кат. номер T4330-50KU T4330-50KU-PW T4330-100KU ApplicationTurbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating.
Used for the removal of nucleic acid from protein samples.Biochem/physiol ActionsTurbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA.
Digests native or heat-denatured DNA and RNA.Unit DefinitionOne unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C.Physical formSupplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaClПараметрыQuality Level 200 recombinant expressed in E. coli form liquid concentration 200,000 units/mL storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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