Microbial Culture Media Sigma-Aldrich

2x YT medium has been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.
Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

250 g in poly bottle

2xYT powder provides:

Standard formulation
An inexpecnsive alternative to liquid

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Quality Level	200,
grade	for molecular biology
sterility	non-sterile
form	powder
pH range	6.8 - 7.2 (3.1% solution)
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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2xYT Medium Y2627-1KG

2xYT EZMix™ powder provides:

Granulated, dust-free format for safer handling and faster mixing
Easy scale-up using larger package size
Standard formulation

Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

EZMix is a trademark of Sigma-Aldrich Co. LLC

grade	for molecular biology
description	nutrient rich, quick dissolve, dust free formulation
sterility	non-sterile
form	powder
composition	Inert binder (EZMix only), 0.6 g/L , NaCl, 5 g/L , Tryptone (pancreatic digest of casein), 16 g/L , Yeast extract, 10 g/L
Featured Industry	Agriculture Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

Formulation for the growth and expansion of the Lyme desease spirochete, Borrelia burgdorferi
BSK-H medium is a standardized complex medium designed to support the growth of the Lyme disease spirochete, Borrelia burgdorferi. To standardize the procedure for isolating and culturing Lyme disease spirochetes, Pollack, RJ et. al. modified the composition of the medium generally used for this purpose (BSK-II) and developed a system for its distribution. This medium contains no gelatin or agarose, and various components are used in proportions that differ from those in BSK-II. Each of the major proteinacious components was screened by substitution in samples of the complete product. The final medium was evaluated for the capacity to grow related spirochetes including Borrelia burgdorferi N40, Guilford, and JD-1 as well as strains of Borrelia hermsii (HS-1) and of Borrelia coriaceae (CO53). Standardized medium, supplemented with prescreened rabbit serum, facilitates comparison of research results between laboratories and may eventually permit definitive clinical diagnosis of Lyme disease based on demonstration of the pathogen. The standardized medium is designated BSK-H.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BSK-H Medium

1. BSK-H Medium is supplied as a sterile-filtered liquid. After thawing, mix well by inverting the bottle prior to use. If the thawed medium will not be used within a few days it is recommended that the medium be refrozen in working aliquots to avoid repeated free-thaw cycles.
2. Prior to use, we recommend that the medium should be supplemented aseptically with prescreened, sterile-filtered serum to a final concentration of 6%. (See note below)
3. Other supplements may be added aseptically as desired. The nature of the supplement may affect storage conditions and shelf-life of the medium.
NOTE: For best results, serum used to supplement the medium should be prescreened for its ability to support the growth of Borrelia burgdorferi. Prescreened, sterilefiltered Rabbit Serum [Product No. R7136] is available from Sigma.

Recommended products

For best results, serum used to supplement the medium should be prescreened for its ability to support the growth of Borrelia burgdorferi. Prescreened, sterile filtered Rabbit Serum [Product No. R7136] is available from Sigma.

Quality Level	400,, 500,
sterility	sterile; sterile-filtered
form	liquid
suitability	suitable for Borrelia burgdorferi (Qualified)
Featured Industry	Food and Beverages
components	NaHCO₃: 2.2 g/L HEPES: 6.0 g/L phenol red: 0.02124 g/L glucose: 6.0 g/L (Dextro)
shipped in	dry ice
storage temp.	20°C

Complete BSK-H Medium was used to culture B. burgdorferi to study the pathology of lyme borreliosis. It may be used in serum supplemented formulation for the growth and expansion of the Lyme desease spirochete, Borrelia burgdorferi.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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BSK-H Medium, Complete

Biochem/physiol Actions

BSK-H medium is a standardized complex medium designed to support the growth of the Lyme disease spirochete, Borrelia burgdorferi. To standardize the procedure for isolating and culturing Lyme disease spirochetes, Pollack, RJ et. al. modified the composition of the medium generally used for this purpose (BSK-II) and developed a system for its distribution. This medium contains no gelatin or agarose, and various components are used in proportions that differ from those in BSK-II. Each of the major proteinacious components was screened by substitution in samples of the complete product. The final medium was evaluated for the capacity to grow related spirochetes including Borrelia burgdorferi N40, Guilford, and JD-1 as well as strains of Borrelia hermsii (HS-1) and of Borrelia coriaceae (CO53). Standardized medium, supplemented with prescreened rabbit serum, facilitates comparison of research results between laboratories and may eventually permit definitive clinical diagnosis of Lyme disease based on demonstration of the pathogen. The standardized medium is designated BSK-H.
Barbour-Stonner-Kelly (BSK) medium is a modified medium for the culture of Borrelia strains that cause lyme borreliosis. BSK medium is a cheaper alternative for the selective culture of B. burgdorferi s.l. strains but not B. afzelii strain.

BSK-H Medium is supplied as a sterile-filtered liquid. After thawing, mix well by inverting the bottle prior to use. If the thawed medium will not be used within a few days it is recommended that the medium be refrozen in working aliquots to avoid repeated free-thaw cycles. Other supplements may be added aseptically as desired. The nature of the supplement may affect storage conditions and shelf-life of the medium.

Quality Level	400,
sterility	sterile-filtered
form	liquid
suitability	suitable for Borrelia burgdorferi
Featured Industry	Food and Beverages
components	phenol red: 0.01997 g/L NaHCO₃: 2.068 g/L HEPES: 5.64 g/L glucose: 5.64 g/L (Dextro)
shipped in	dry ice
storage temp.	20°C

Hannahans Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. The 0.4% dextrose composition provides the E. coli carbon and energy needed to mend perforations and for replication.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Hanahans Broth (SOB Medium)

Hanahans Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.

Hannahans Broth (SOB) powder provides:

Standard formulation
An inexpensive alternative to liquid

Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Other Notes

Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.

Quality Level	100,
grade	for molecular biology
description	recommended for cultivation of recombinant E. coli.
sterility	non-sterile
form	powder
composition	KCl, 0.186 g/L , MgSO₄, 2.4 g/L , NaCl, 0.5 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L
pH range	6.8 - 7.2 (2.8% solution)
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

Dehydrated Luria-Bertani (LB) broth is called the Lennox LB, which has amino acids as carbon source.
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Personal Protective Equipment	Eyeshields,, Gloves,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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LB Broth (Lennox)

LB Broth (Lennox) has been used for culturing Escherichia coli and bacterial artificial chromosomes (BACs).
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

100, 500 tablets in poly bottle

Lennox LB tablets provide:

Convenient small package to eliminate weighing
Fast preparation – no stirring required

10 g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents

Add each 1.1g tablet in 48.3mL water (no stirring required). Autoclave for 20 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Quality Level	200,
grade	for molecular biology
sterility	non-sterile
form	tablet
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

Lurias low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low salt formulation.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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LB Broth (Luria low salt)

LB Broth (Luria low salt) has been used as a diluting agent for the preparation of dilution series of free levofloxacin (LVF) and LVF encircled by nanohydrogel (LVF-NHs).
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Lurias low salt LB powder provides:

Standard formulation
A budget-friendly alternative to liquid
Easy scale-up using larger package sizes

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Quality Level	200,
grade	for molecular biology
sterility	non-sterile
composition	NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
pH range	6.8 - 7.2 (1.55% solution)
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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LB Broth with agar (Lennox)

Lennox LB powder with agar provides:

A budget-friendly alternative to pre-poured plates
Easy scale-up using larger package sizes
Standard formulation

1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.

Quality Level	200
grade	for molecular biology
sterility	non-sterile
composition	Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

LB Broth with agar (Luria low salt) has been used for culturing E. coli DH10B cells and in the isolation of diazotrophic bacteria.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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LB Broth with agar (Luria low salt)

Lurias low salt LB powder with agar provides:

Easy scale-up using larger package sizes
A budget-friendly alternative to pre-poured plates
Standard formulation

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

Quality Level	200,
grade	for molecular biology
sterility	non-sterile
form	powder
composition	Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
pH range	6.8 - 7.2 (3.05% solution)
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

Millers LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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LB Broth with agar (Miller)

eCl@ss:	42040102
NACRES:	NA.85

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
LB Broth with agar (Miller) has been used for the generation of single colony for Pseudomonas putida KT2440, plating E. coli and for the generation of pure colonies of C14 and MC8M6 of bacterial strains.

250 g in poly bottle
1 kg in poly bottle

Millers LB powder with agar provides:

Easy scale-up using larger package sizes
A budget-friendly alternative to pre-poured plates
Standard formulation

1. Suspend 40 g in 1L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into sterile petri dishes.
To prepare the medium of Luria and Burrows: Add 1 g glucose to medium and proceed with preparation instructions above.
To prepare the medium of Luria, Adams and Ting (also known as LC agar): Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Add 40g powder to 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired).

Quality Level	200,
grade	for molecular biology
sterility	non-sterile
form	powder
composition	Agar, 15 g/L , NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
pH range	6.8 - 7.2 (4% solution)
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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M9, Minimal Salts, 5X

M9, Minimal Salts, 5X has been used:

in a selective agar medium for the isolation of sulphate reducing bacteria
in GMML minimal media to propagate phage-resistant DH10B cells
in M9 minimal medium which is used for cell motility assay

M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F-containing bacteria strains for M13 propagation.

1 kg in poly bottle

M9 powder provides:

Standard formulation
A budget-friendly alternative to liquid

1. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121°C.
This convenient 5x concentrate can be stored and diluted as needed to prepare 5 L of 1x M9 minimal salts.
For M9 minimal medium:
1. Aseptically dilute 200 ml of M9 minimal salts, 5x concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.

Make a 5x concentrated stock solution by stirring to suspend 56.4g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).

Quality Level	200
grade	for molecular biology
sterility	non-sterile
form	powder
composition	KH₂PO₄, 15 g/L , NaCl, 2.5 g/L , Na₂HPO₄, 33.9 g/L , NH₄Cl, 5 g/L
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

NZ Amine is a growth medium used for the cultivation of lambda bacteriophage. This nutrient-rich broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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NZ Amine® Broth

Suitable for non-selective cultivation of lambda bacteriophage.

NZ Amine powder provides:

Standard formulation
Convenient package sizes
Easy scale-up using larger package sizes
A budget-friendly alternative to liquid

1. Suspend 15.94 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Stir to suspend 16 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

N-Z-Amine is a registered trademark of Kerry Group

Quality Level	100,
grade	for molecular biology
sterility	non-sterile
form	powder
composition	MgSO₄, 1 g/L , NaCl, 5 g/L , Tryptone, 10 g/L
pH range	6.3 - 7.2 (1.6% solution)
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

NZCYM is a microbial growth medium used for the cultivation of E. coli and lambda bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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NZCYM Broth

Suitable for non-selective cultivation of lambda bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

250 g in poly bottle
1 kg in poly bottle

NZCYM powder provides:

Standard formulation
Convenient package sizes
A budget-friendly alternative to liquid

Stir to suspend 22 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Quality Level	200,
grade	for molecular biology
sterility	non-sterile
form	powder
composition	Casamino Acids, 1 g/L , MgSO₄, 15 g/L , NaCL, 5 g/L , Tryptone, 10 g/L , Yeast extract, 5 g/L
pH range	6.8 - 7.2 (2.2% solution)
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for -galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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S-Gal®/LB Agar Blend

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® (sodium salt) is water-soluble, autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
S-Gal®/LB Agar Blend has been used for the identification of recombinant bacterial colonies.

More intense color contrast than X-gal
Water-soluble and autoclavable for easiest use
Convenient, pre-mixed media

Ingredients (g/L)
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03

Principle

When S-Gal® is cleaved by -galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.

Suspend contents of one packet in 500 ml distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124°C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52°C.

Other Notes

The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.

S-GAL is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
grade	for molecular biology
sterility	non-sterile
form	powder
suitability	suitable for -galactosidase test, nonselective for Escherichia coli, nonselective for coliforms
Featured Industry	Food and Beverages
storage temp.	room temp

SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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SOC Medium

SOC Medium is a rich media used primarily in the recovery step of Escherichia coli competent cell transformations. Use of SOC maximizes the transformation efficiency of competent cells.

SOC liquid provides:

Filter-sterilized, ready-to-use format
Convenient package sizes
Standard formulation

1. Suspend 31.3 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Quality Level	500
grade	for molecular biology
sterility	0.2 µm filtered
form	liquid
composition	Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO₄, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L
Featured Industry	Agriculture Food and Beverages
storage temp.	2-8°C
suitability	nonselective for Escherichia coli, nonselective for coliforms

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Terrific Broth

Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:

as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)

1 kg in poly bottle

Terrific Broth EZMix™ powder provides:

Granulated, dust-free format for safer handling and faster mixing
A budget-friendly alternative to liquid
Modified formulation (without glycerol)
Easy scale-up using larger package sizes

*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4

Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

EZMix is a trademark of Sigma-Aldrich Co. LLC

Quality Level	200,
grade	for molecular biology
description	TB; quick dissolve, dust-free formulation
sterility	non-sterile
form	powder
Featured Industry	Agriculture Food and Beverages
storage temp.	room temp
suitability	nonselective for Escherichia coli, nonselective for coliforms

Yeast Nitrogen Base is considered ideal for culturing wild type yeast. The presence of amino acid is known to prevent selectable marker utilization. Hence, this media is formulated without amino acids and is useful for cloning and manipulation of yeast artificial chromosome.
Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Yeast Nitrogen Base Without Amino Acids

Yeast Nitrogen Base Without Amino Acids has been used:

in synthetic complete supplement mixture (SC)-Leu media used for Y. lipolytica culturing and characterization
as a component of selective liquid media for uracil auxothrophic growth in yeast
to evaluate the transformation efficiencies of S. cerevisiae strains, under different nutrient conditions

Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.

Component, amount (µg/L unless indicated)
Nitrogen Sources:
Ammonium sulfate, 5.0 g/L
Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400
Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400
Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

1. Prepare a 10x stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10x stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.

Quality Level	200
grade	for molecular biology
sterility	non-sterile
form	powder
application(s)	microbe id \| utilization test: suitable
pH range	5.2 - 5.6 (0.67% solution)
Featured Industry	Food and Beverages
storage temp.	room temp
suitability	nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp., yeasts

250 g in poly bottle
1 kg in poly bottle

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US)
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Yeast Synthetic Drop-out Medium Supplements

MDL number: MFCD01095679

NACRES: ND.02

Кат. номер

Y1501-1G-PW

Y1501-20G-PW

Y1501-20G

Y1501-1G-KC

Y1501-1G

Packaging
20 g in poly bottle
Application
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Components
The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids are present at a concentration of 76 mg/L except for leucine, which is present at 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
Quantity
1.92 g will supplement 1 liter of medium.
Параметры

Quality Level 100,

Featured Industry Food and Beverages

Safety Information

Personal Protective Equipment Eyeshields,, Gloves,, type N95 (US),

RIDADR NONH for all modes of transport

WGK Germany WGK 1

Flash Point F Not applicable

Flash Point C Not applicable

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YPD Agar

eCl@ss:	42040102
NACRES:	NA.75

A solid medium for the propagation of yeast.

1. Suspend 65 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.

Quality Level	200,
composition	Agar, 15 g/L , Bacteriological peptone, 20 g/L , Glucose, 20 g/L , Yeast extract, 10 g/L
Featured Industry	Food and Beverages
suitability	nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp.