Immunodetection Reagents & Substrates Sigma-Aldrich
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CDP-Star® Chemiluminescent Substrate
Empirical Formula (Hill Notation): C18H19Cl2O7Na2P CAS Number: 160081-62-9 Molecular Weight: 495.20 MDL number: MFCD02094611 PubChem Substance ID: 329774818 NACRES: NA.83
Кат. номер C0712-100ML C0712-100ML-KC ApplicationCDP-Star® is a sensitive, chemiluminescent substrate for alkaline phosphatase that allows for the rapid, reproducible detection of alkaline phosphatase-labeled molecules in Northern, Southern, and Western blotting applications. Detection of alkaline phosphatase-labeled molecules with CDP-Star is extremely sensitive as a result of low background luminescence coupled with high intensity and prolonged light output from the enzyme catalysis. Following exposure to alkaline phosphatase-labeled molecules, maximum light emission occurs at approximately 60 minutes and continues for up to 24 hours, allowing for multiple film exposures and/or the sensitive detection of targets present in small amounts. For convenience, CDP-Star is supplied as a 0.25 mM, ready-to-use aqueous solution (i.e. no dilution is necessary). CDP-Star functions on both neutral and positively-charged nylon, giving the reagent added application versatility.Features and BenefitsCDP-Star® is supplied as a 0.25 mM ready-to-use aqueous solution for use in a variety of membrane-based applications. CDP-Star functions on both neutrally-charged and positively-charged nylon, giving the reagent added application versatility.CautionStore at 2-8 °C protected from light.Legal InformationCDP-Star® is a trademark of Tropix, Inc. Bedford, MA, USA and covered under U.S. Patent 5,326,882.
CDP-Star is a registered trademark of Tropix, Inc.ПараметрыQuality Level 200 assay 75% (HPLC) form liquid usage sufficient for 20 blots detection of alkaline phosphatase-labeled molecules (10 x 10 cm) concentration 0.25 mM in H2O storage temp. 2-8°C SMILES string Cl[C@]1(C2)C[C@H]3C4(OOC4(C5=CC(OP([O-])([O-])=O)=C(Cl)C=C5)OC)[C@@H]2C[C@@H](C1)C3 InChI 1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;2*+1/p-2/t10-,11-,12+,16-,17?,18?;; InChI key UKWLRLAKGMZXJC-VCCHNUGISA-L
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Chemiluminescent Peroxidase Substrate for ELISA
MDL number: MFCD08705424 NACRES: NA.83
Кат. номер CPS260-1KT-PW CPS260-1KT ApplicationTwo component liquid substrate system with superior sensitivity, signal-to-noise, and linearity.ПараметрыQuality Level 100, usage sufficient for 60 mL substrate storage temp. 2-8°C
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Chemiluminescent Peroxidase Substrate-1
MDL number: MFCD03456389
Кат. номер CPS160-1KT-PW CPS160-1KT ApplicationTwo component liquid peroxidase substrate system for Western blot detection. Picomole detection with superior signal-to-noise ratio. Component ratios can be adjusted for optimal signal duration, sensitivity and background.Параметрыusage sufficient for 60 mL substrate storage temp. 2-8°C
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Chemiluminescent Peroxidase Substrate-3 CPS350-1KT
MDL number: MFCD08705424 NACRES: NA.83 ApplicationChemiluminescent Peroxidase Substrate-3 has been used for the quantification of aptamer binding. It has also been used in endopeptidase assay to determine the enzymatic activity of botulinum neurotoxins (BoNTs).
CPS3 formulations are economically priced and outperform the most commonly used competitors′ products. This two component system provides low nanogram detection with superior signal-to-noise ratio. In addition, the components are stable after mixing, adding to the flexibility of usage.Параметрыform solution Quality Level 200, usage sufficient for 50 mL substrate storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Chemiluminescent Peroxidase Substrate-3 CPS3100-1KT
MDL number: MFCD08705424 NACRES: NA.83 ApplicationCPS3 formulations are economically priced and outperform the most commonly used competitors′ products. This two component system provides low nanogram detection with superior signal-to-noise ratio. In addition, the components are stable after mixing, adding to the flexibility of usage.Параметрыform solution Quality Level 100, usage sufficient for 100 mL substrate storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Chemiluminescent Peroxidase Substrate-3 CPS3500-1KT
MDL number: MFCD08705424 NACRES: NA.83 ApplicationCPS3 formulations are economically priced and outperform the most commonly used competitors′ products. This two component system provides low nanogram detection with superior signal-to-noise ratio. In addition, the components are stable after mixing, adding to the flexibility of usage.Параметрыform solution Quality Level 100, usage sufficient for 500 mL substrate storage temp. 2-8°C
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Gelatin blocking buffer G7663-1L
NACRES: NA.25 General descriptionGelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).Packaging1 L in poly bottleApplicationSuitable for use as a non-specific blocking agent for Western blots.ReconstitutionDissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.ПараметрыQuality Level 100 grade for molecular biology form powder blend storage temp. room temp
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Phosphate buffered saline
MDL number: MFCD00131855 NACRES: NA.25
Кат. номер P4739-10PAK P4739-10VL ApplicationPhosphate buffered saline has been used as a blocking buffer in dot blot or Western blot applications.ПараметрыQuality Level 100 form powder (dry milled) composition nonfat dry milk , potassium chloride, 76.5% , potassium phosphate monobasic anhydrous, 0.3% , sodium chloride, 12.2% , sodium phosphate dibasic, 10.7% pH 7.3
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
SIGMAFAST™ 3,3-Diaminobenzidine tablets
CAS Number: 91-95-2 MDL number: MFCD00007725 PubChem Substance ID: 24893872 NACRES: NA.83
Кат. номер D4418-5SET D4418-50SET ApplicationDeveloped for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packetsReconstitutionEach tablet set dissolved in 15 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.Legal InformationSIGMAFAST is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 form tablet solubility water: 1 tablet/5 mL storage temp. 20°C SMILES string Nc1ccc(cc1N)-c2ccc(N)c(N)c2 InChI 1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2 InChI key HSTOKWSFWGCZMH-UHFFFAOYSA-N
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H302 - H315 - H319 - H335 - H341 - H350 pcodes P202 - P261 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313 Target Organs Respiratory system RIDADR UN 3316 9 WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
TMB Enhanced One Component HRP Membrane Substrate
NACRES: NA.83
Кат. номер T9455-100ML-PW T9455-1L T9455-100ML T9455-1L-PW General descriptionTMB is a substrate for horseradish peroxidase. The substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. This substrate is not recommended for microwell or immunohistochemical applications.CautionStable at least one year at 2-8 °C.Physical formReady-to-use.ПараметрыQuality Level 200 form solution storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Western Blocker™ Solution
NACRES: NA.25
Кат. номер W0138-400ML W0138-400ML-KC W0138-PH W0138-400ML-PW W0138-500ML General descriptionWestern Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.ApplicationWestern Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.Features and Benefits- Does not inhibit weak HRP signals, as many milk-based blockers do.
- Can be used both as a blocking component and as a probing component.
- Supplied ready-to-use; no preparative step is required.
Legal InformationWestern Blocker is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 shelf life 2 yr storage temp. 2-8°C
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