Hygromycin B from Streptomyces hygroscopicus

Empirical Formula (Hill Notation):	C20H37N3O13
CAS Number:	31282-04-9
Molecular Weight:	527.52
EC Number:	250-545-5
MDL number:	MFCD06795479
PubChem Substance ID:	329815270
NACRES:	NA.72

Кат. номер

H9773-100MG

H9773-250MG

H9773-50MG

H9773-PH

H9773-250MG-PW

H9773-50MG-PW

H9773-100MG-PW

Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
Chemical structure: aminoglycoside
Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 µg/mL, specifically at 100 µg/mL for prokaryotes, 200 µg/mL for lower eukaryotes and 150-400 µg/mL for higher eukaryotes.
Hygromycin B from Streptomyces hygroscopicus is used for gene cloning and ectopic expression. It is also used in MS medium for the in vitro screening of transgenic plants.
Hygromycin B is used in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. It is also adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans. It can be inactivated by Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) through phosphorylation. The amino function of this antibiotic may be involved in a general acid-base catalysis performed by the ribozyme, acting as proton acceptors/donors.
Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.
Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.
Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.
This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

biological source	Streptomyces hygroscopicus
Quality Level	200,
product line	BioReagent
assay	60% (HPLC)
form	lyophilized powder
purified by	ion-exchange chromatography
application(s)	cell culture | plant: suitable
color	faintly brown to brown, white to beige
A	(1) 7.1, (2) 8.8
solubility	H2O: soluble 50 mg/mL, ethanol: soluble, methanol: soluble
Featured Industry	Agriculture
Mode of action	protein synthesis | interferes
antibiotic activity spectrum	fungi
storage temp.	2-8°C
SMILES string	CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
InChI	1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
InChI key	GRRNUXAQVGOGFE-XKIAHZFYSA-N

pictograms	GHS06
signalword	Danger
hcodes	H300 + H310 + H330
pcodes	P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
RIDADR	UN3462 - class 6.1 - PG 1 - Toxins, extracted from living sources, solid, n.o.s., HI: all (not BR)
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable