Glycosidases Sigma-Aldrich
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(23) Neuraminidase from Streptococcus pneumoniae
CAS Number: 9001-67-6 Enzyme Commission (EC) Number: 3.2.1.18 ( BRENDA | IUBMB ) MDL number: MFCD01092203 NACRES: NA.32
PackagingКат. номер N7271-.2UN-PW N7271-.2UN
.2 unit in poly bottle
Provided with 5x reaction buffer (250 mM sodium phosphate, pH 6.0).
Biochem/physiol Actions
Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.
Unit Definition
One unit will hydrolyze 1µmole of 4-methylumbelliferyl -D-N-acetylneuraminide per min at pH 5.0 at 37 °C.
Physical form
Solution in 50 mM sodium phosphate, pH 7.5
Preparation Note
Expressed in glycosidase-free hosts.Параметрыform buffered aqueous solution Quality Level 100, concentration 5 units/mL foreign activity -Galactosidase, -mannosidase, -hexosaminidase, -fucosidase, and proteases, none detected shipped in wet ice storage temp. 2-8°C Gene Information Streptococcus pneumoniae R6 ... nanB(933504)
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(23,6) Neuraminidase from Clostridium perfringens (C. welchii)
Enzyme Commission (EC) Number: 3.2.1.18 ( BRENDA | IUBMB ) MDL number: MFCD01092201 NACRES: NA.32
Biochem/physiol ActionsКат. номер N5521-.4UN-PW N5521-.4UN
Releases α(2→3)- and α(2→6)-linked N-acetylneuraminic acid from complex oligosaccharides.
Packaging
Provided with 5x reaction buffer (250 mM sodium phosphate, pH 6.0).
Unit Definition
One unit will hydrolyze 1 µmole of 4-methylumbelliferyl -D-N-acetylneuraminide per min at pH 5.0 at 37 °C
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, and 25 mM NaCl.
Preparation Note
Expressed in glycosidase-free hosts.ПараметрыQuality Level 200, recombinant expressed in E. coli form buffered aqueous solution specific activity 250 units/mg protein mol wt ~41 kDa foreign activity proteases, none detected shipped in wet ice storage temp. 2-8°C
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(23,6,8,9) Neuraminidase from Arthrobacter ureafaciens
CAS Number: 9001-67-6 Enzyme Commission (EC) Number: 3.2.1.18 ( BRENDA | IUBMB ) MDL number: MFCD00131711 NACRES: NA.32
Кат. номер N8271-25UL-PW N8271-25UL N8271-.2UN-PW N8271-.2UN Biochem/physiol ActionsReleases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.PackagingProvided with 5x reaction buffer (250 mM sodium phosphate, pH 6.0).Unit DefinitionOne unit will hydrolyze 1 µmole of 4-methylumbelliferyl -D-N-acetylneuraminide per min at pH 5.0 at 37 °C.Physical formSolution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.Preparation NoteExpressed in glycosidase-free hosts.ПараметрыQuality Level 200 recombinant expressed in E. coli form buffered aqueous solution specific activity 135 units/mg protein mol wt 88 kDa, 95 kDa foreign activity -Galactosidase, -mannosidase, -hexosaminidase, -fucosidase, and proteases, none detected shipped in wet ice storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
-1(2,3,4)-Fucosidase solution from Xanthomonas sp.
CAS Number: 9037-65-4 Enzyme Commission (EC) Number: 3.2.1.51 ( BRENDA | IUBMB ) MDL number: MFCD01632935 NACRES: NA.32
Unit DefinitionКат. номер F1924-1VL F1924-1VL-PW
One unit will hydrolyze 1 μmole fucose from 3-fucosyllactose per min at pH 5.0 at 37 °C.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaClПараметрыform buffered aqueous solution Quality Level 200, specific activity 0.5 units/mg protein packaging vial of 0.004 unit shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
-1(3,4)-Fucosidase solution from Xanthomonas sp.
CAS Number: 9037-65-4 Enzyme Commission (EC) Number: 3.2.1.51 ( BRENDA | IUBMB ) EC Number: 232-914-2 MDL number: MFCD00130491
Biochem/physiol ActionsКат. номер F3023-1VL F3023-1VL-PW
Releases non-reducing, terminal α-1,3-fucose and α-1,4-fucose from complex carbohydrates.
Unit Definition
One unit will release 1.0 μmole of fucose from Lewis X trisaccharide, 4-methylumbelliferyl glycoside per min at pH 5.0 at 37 °C.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaClПараметрыform buffered aqueous solution Quality Level 200, specific activity 2 units/mg protein packaging vial of 0.02 unit concentration 0.5 unit/mL foreign activity -galactosidase, -mannosidase, -hexosaminidase, neuraminidase, -1,2-fucosidase and proteases, none detected shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
-1,6-Fucosidase solution from Elizabethkingia miricola
CAS Number: 9037-65-4 EC Number: 232-914-2 MDL number: MFCD01092200
PackagingКат. номер F6272-.04UN-PW F6272-.04UN
0.04 unit in glass bottle
Biochem/physiol Actions
For reactions with complex carbohydrates, the substrate must be labeled with aminonaphthalenetrisulfonic acid (ANTS).
Unit Definition
One unit will release 1.0 µmole of methylumbelliferone from 4-methylumbelliferyl -L-fucoside per min at pH 5.0 at 37 °C
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaClПараметрыQuality Level 100 recombinant expressed in E. coli form buffered aqueous solution specific activity 1.8 units/mg protein UniProt accession no. P04066,","Q9BTY2, foreign activity -galactosidase, -mannosidase, -hexosaminidase, neuraminidase and proteases, none detected shipped in wet ice storage temp. 2-8°C Gene Information human ... FUCA1(2517), FUCA2(2519)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
-Galactosidase, positionally specific from Escherichia coli
CAS Number: 9025-35-8 Enzyme Commission (EC) Number: 3.2.1.22 ( BRENDA | IUBMB ) MDL number: MFCD00130486
Biochem/physiol ActionsКат. номер G7163-60UN-PW G7163-60UN
Cleaves α(1→3)- and α(1→6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. It is particularly efficient for removing α-linked galactose under conditions where the pH must be neutral or above, for example, with live cells.
Unit Definition
One unit will hydrolyze 1 µmole of p-nitrophenyl -D-galactopyranoside per min at pH 6.5 at 25 °C.
Physical form
This product is a sterile-filtered aqueous buffered solution.ПараметрыQuality Level 200, recombinant expressed in E. coli form buffered aqueous solution specific activity 20 units/mg protein mol wt 80 kDa shipped in wet ice storage temp. 2-8°C Gene Information Escherichia coli CFT073 ... melA(1037886)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
-Glucosidase from Saccharomyces cerevisiae G0660-750UN
CAS Number: 9001-42-7 Enzyme Commission (EC) Number: 3.2.1.20 ( BRENDA | IUBMB ) EC Number: 232-604-7 MDL number: MFCD00081321 NACRES: NA.32 General descriptionWe are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles,.
Packaging
750 units in poly bottleApplicationFor the determination of -amylase and the synthesis of various 1-O-sucrose and 1-O-fructose esters
Biochem/physiol Actions
Hydrolysis of terminal, non-reducing 14-linked D-glucose residues with release of D-glucose.
Unit Definition
One unit will liberate 1.0 µmole of D-glucose from p-nitrophenyl -D-glucoside per min at pH 6.8 at 37 °C.
Physical form
Lyophilized powder containing potassium phosphate buffer salt pH 7.15 and Approx. 70% lactose
Analysis Note
Protein determined by biuret.ПараметрыQuality Level 200 recombinant expressed in proprietary host form lyophilized powder specific activity 100 units/mg protein greener alternative product characteristics Waste Prevention
Design for Energy Efficiency.UniProt accession no. P38158,","P53341, greener alternative category Enabling, shipped in wet ice storage temp. 2-8°C Gene Information bakers yeast ... MAL12(853209), MAL32(852602)
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-Mannosidase from Helix pomatia
CAS Number: 9025-43-8 Enzyme Commission (EC) Number: 3.2.1.25 ( BRENDA | IUBMB ) MDL number: MFCD00130638 NACRES: NA.32
Unit DefinitionКат. номер M9400-5UN M9400-1UN
One unit will hydrolyze 1 µmole of p-nitrophenyl--D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.
Physical form
Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0Параметрыform ammonium sulfate suspension Quality Level 200, quality crude extract mol wt 94 kDa concentration 5-30 units/mL foreign activity -N-acetylglucosaminidase and -mannosidase <1.0% storage temp. 2-8°C
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-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean)
CAS Number: 9012-33-3 Enzyme Commission (EC) Number: 3.2.1.52 ( BRENDA | IUBMB ) EC Number: 232-730-2 MDL number: MFCD00081402 NACRES: NA.54
Кат. номер A2264-25UN A2264-10UN A2264-1VL-KC A2264-5UN A2264-1VL A2264-100UN Application-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl--D-glucosaminides and N-acetyl--Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of -GlcNAc and -GalNAcc. It may be a useful tool to study Alzheimers Disease . Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms .Biochem/physiol ActionsThis enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.Unit DefinitionOne unit will hydrolyze 1.0 µmole of p-nitrophenyl N-acetyl--D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 25 °C.Physical formSuspension in 2.5 M (NH4)2SO4, pH 7.0Analysis NoteAt pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0.ПараметрыQuality Level 200 form ammonium sulfate suspension specific activity 10 units/mg protein foreign activity - and -galactosidase, and -L-fucosidase 0.1%, -mannosidase 0.2% storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
-N-Acetylglucosaminidase from Streptococcus pneumoniae
CAS Number: 9012-33-3 Enzyme Commission (EC) Number: 3.2.1.52 ( BRENDA | IUBMB ) EC Number: 232-730-2 MDL number: MFCD00081402 NACRES: NA.32
Кат. номер A6805-1VL-PW A6805-1VL Application-N-Acetylglucosaminidase from Streptococcus pneumoniae has been used in the removal of O-linked sugars from inositol 1,4,5-trisphosphate (InsP3) receptor type I. It has also been used in coating PVDF strips for amelogenin trityrosyl motif peptide (ATMP) and keratin 5 binding studies.
Biochem/physiol Actions
-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the 1-3 and 1-6 linkages present in mucins. It belongs to the glycoside hydrolase family 85 (GH85). Also called endo--N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides and acts on fucosylated N-glycan core. Its deglycosylation functionality is exploited in studying functions of glycoproteins.
Specific for GlcNAc; not significantly active with GalNAc
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.
Unit Definition
One unit will hydrolyze 1.0 µmole of p-nitrophenyl N-acetyl--D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 5 at 37 °C.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaClПараметрыQuality Level 100, recombinant expressed in E. coli form buffered aqueous solution specific activity 80 units/mg protein packaging vial of 1.0 unit foreign activity -galactosidase, -mannosidase, -fucosidase, neuraminidase, and proteases, none detected (Enzyme is expressed in glycosidase-free host.) shipped in wet ice storage temp. 2-8°C Gene Information Streptococcus pneumoniae R6 ... lytB(934406)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli
CAS Number: 9012-33-3 Enzyme Commission (EC) Number: 3.2.1.30 ( BRENDA | IUBMB ) MDL number: MFCD00081402
PackagingКат. номер 110116-1U 110116-1UCN
1 u in Plastic ampoule
Warning
Toxicity: Standard Handling (A)Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C form liquid Quality Level 100, specific activity 35 units/mL, 80 units/mg protein Торговая марка Calbiochem® storage condition do not freeze foreign activity Endo--N-acetylgalactosaminidase, - and -galactosidase, neuraminidases, proteases, none detected shipped in wet ice storage temp. 2-8°C
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli
CAS Number: 9001-67-6 Enzyme Commission (EC) Number: 3.2.1.18 ( BRENDA | IUBMB )
Кат. номер 480716-250MIU 480716-250MIUCN General descriptionRecombinant, Arthrobacter ureafaciens 2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.
Note: 1 mU = 1 milliunit.
Packaging
250 miu in Plastic ampoule
Warning
Toxicity: Harmful (C)
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).
Physical form
In 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.Other NotesMeans, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C form liquid Quality Level 100, specific activity 40 units/mg protein, 5 units/mL Торговая марка Calbiochem® storage condition do not freeze foreign activity N-acetylglucosaminidase, -fucosidase, - and -galactosidase, - and -mannosidase, proteases, none detected shipped in wet ice storage temp. 2-8°C
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Agarase from Pseudomonas atlantica
CAS Number: 37288-57-6 Enzyme Commission (EC) Number: 3.2.1.81 ( BRENDA | IUBMB ) MDL number: MFCD00130365 eCl@ss: 42040102 NACRES: NA.26
Кат. номер A6306-1KU-PW A6306-5KU A6306-1KU A6306-5KU-PW Packaging1000 units in serum bottleUnit DefinitionOne unit will produce 1.0 µg of reducing sugar (measured as D-galactose) from agar per min at pH 6.0 at 40 °C.Physical formContains phosphate buffer salts. May contain bovine serum albumin to standardize protein contentПараметрыform lyophilized powder Quality Level 200 specific activity 5,000 units/mg protein (Lowry) storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Chondroitinase ABC from Proteus vulgaris
CAS Number: 9024-13-9 Enzyme Commission (EC) Number: 4.2.2.4 ( BRENDA | IUBMB ) EC Number: 232-777-9 MDL number: MFCD00130795 NACRES: NA.54
Кат. номер C2905-10UN-PW C2905-2UN-PW C2905-5UN C2905-10UN C2905-2UN C2905-5UN-PW ApplicationChondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.Packaging2, 5, 10 units in glass bottle
Packages based on chondroitinase C activity.Biochem/physiol ActionsChondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)--D-hexosaminyl and (1-3)--D-glucuronosyl (or (1-3)--L-iduronosyl) linkages to disaccharides containing 4-deoxy--D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.Unit DefinitionOne unit will liberate 1.0 µmole of a mixture of 2-acetamido-2-deoxy-3-O-(-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose and 1.0 µmole of 2-acetamido-2-deoxy-3-O-(-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate from shark cartilage, per min at pH 8.0 at 37 °C.Physical formLyophilized powder containing Tris buffer saltsPreparation NoteReconstitute in a 0.01% bovine serum albumin aqueous (BSA) solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% BSA. Solutions should be freshly prepared.ПараметрыQuality Level 300 form lyophilized powder specific activity 0.3-3 units/mg solid mol wt ~120 kDa by gel filtration composition Protein, ~35% biuret Featured Industry Diagnostic Assay Manufacturing storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Chondroitinase AC from Flavobacterium heparinum
CAS Number: 9047-57-8 Enzyme Commission (EC) Number: 4.2.2.5 ( BRENDA | IUBMB ) MDL number: MFCD00130796 NACRES: NA.54
Кат. номер E2039-10UN E2039-10UN-PW ApplicationChondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
Highly purified to remove interferring β-glucuronidase and protease activities for use in the hydrolysis of chondroitin sulfate pror to HPLC analysis. β-Glucuronidase activity: ≤2,000 femtomole/min/mg protein, Protease activity: ≤2,000 femtomole/min/mg proteinUnit Definition1 unit is defined as the amount of enzyme that will liberate 1.0 µmole per minute of unsaturated disaccharides from chondroitin sulfate A at pH 6.7 at 37 °C as measured by the change in A232. The µ for the reaction product -Di-4S (chondroitin sulfates A and B) is 5.1 and 5.5 for -Di-6S (chondroitin sulfate C).ПараметрыQuality Level 200 recombinant expressed in E. coli assay 90% (SDS-PAGE) form lyophilized solid specific activity 200 units/mg protein shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Chondroitinase B from Flavobacterium heparinum
CAS Number: 52227-83-5 MDL number: MFCD00166444 NACRES: NA.54
Кат. номер C8058-50UN-PW C8058-PH C8058-50UN ApplicationChondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.
The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide. It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.
Biochem/physiol Actions
Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co2+, Fe3+ and Ba2+ also inhibit its activity.
Unit Definition
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.ПараметрыQuality Level 200, form lyophilized powder (with BSA as stabilizer) shipped in dry ice storage temp. 20°C
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Chondroitinase C from Flavobacterium heparinum
CAS Number: 60184-91-0 MDL number: MFCD00212904 NACRES: NA.54
Кат. номер C0954-75UN-PW C0954-PH C0954-75UN General descriptionEnzyme Commission (EC) Number 4.2.2.x
Biochem/physiol Actions
Chondroitinase C acts upon chondroitin sulfate C, releasing unsaturated 6-sulfated disaccharides. The enzyme also acts on hyaluronic acid, producing unsaturated nonsulfated disaccharides. The enzyme is strongly inhibited by NaCl, Fe2+, Co2+ and phosphate ions.
Unit Definition
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 8.0 at 25 °C using chondroitin sulfate C as substrate.Параметрыform lyophilized powder Quality Level 200, specific activity 200 units/mg solid storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endo--galactosidase from Bacteroides fragilis
Enzyme Commission (EC) Number: 3.2.1.103 ( BRENDA | IUBMB ) MDL number: MFCD00131433 NACRES: NA.54
Кат. номер G6920-.5UN-PW G6920-.5UN ApplicationEndo-β-galactosidase was used in fractional protein isolation. It was used for deglycosylation in glycoproteomics of the endothelial secretome of human endothelial cells.
Packaging
0.5 unit in glass insert
Biochem/physiol Actions
Internal (1-4) galactose linkages in unbranched, repeating poly-Nacetyllactosamine [GlcNAc (1-3)Gal (1-4)] structures are the preferred substrate.
Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may reduce or completely inhibit cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharides of the neolacto-group are cleaved at greatly reduced rates depending on the deviation from the preferred substrate. For example, Gal (1-3)GlcNAc (1-3) Gal (1-4)Glc is cleaved at 5X10-5 the rate of keratan sulfate
-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of -galactosidase, in a feedback loop.
Unit Definition
One unit will release 1.0 μmole of reducing sugar from bovine corneal keratan sulfate per minute at 37 °C, pH 5.8.
Physical form
Aseptically filled solution in 20 mM Tris-HCl, pH 7.5ПараметрыQuality Level 100, recombinant expressed in E. coli sterility aseptically filled form buffered aqueous solution specific activity 140 units/mg protein mol wt 32 kDa storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endo--Galactosidase, Bacteroides fragilis, Recombinant, E. coli
Enzyme Commission (EC) Number: 3.2.1.103 ( BRENDA | IUBMB )
Кат. номер 345811-50UL 345811-50ULCN General descriptionRecombinant, Bacteroides fragilis endo--galactosidase expressed in E. coli. Endo--Galactosidase is an enzyme that hydrolyzes internal -galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
Recombinant, Bacteroides fragilis endo--galactosidase expressed in E. coli. Endo--Galactosidase is an enzyme that hydrolyzes internal -galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
Packaging
50 μL in Plastic ampoule
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
Physical form
Sterile-filtered in 20 mM Tris-HCl, pH 7.5.
Reconstitution
There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.Other NotesMurata, T., et al. 2003. Eur. J. Biochem.270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem.157, 365.
Scudder, P., et al. 1984. J. Biol. Chem.259, 6586.
Scudder, P., et al. 1983. Biochem. J.213, 485.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C Quality Level 100, form liquid specific activity 14 U/mL, 140 units/mg protein Торговая марка Calbiochem® storage condition OK to freeze shipped in ambient storage temp. 2-8°C
Safety InformationWGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endo--N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli
CAS Number: 59793-96-3 Enzyme Commission (EC) Number: 3.2.1.97 ( BRENDA | IUBMB )
Кат. номер 324716-50MIU 324716-50MIUCN General descriptionRecombinant, Streptococcus pneumoniae Endo--N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Gal1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides. For carbohydrates containing sialic acid or fucose, pretreatment with neuraminidase or fucosidase is required.
Note: 1 mU = 1 milliunit.
Recombinant, Streptococcus pneumoniae Endo--N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Gal1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides.
Packaging
50 miu in Plastic ampoule
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside per min at 37°C, pH 5.0.
Physical form
In 50 mM sodium phosphate buffer, pH 7.5.Other NotesWang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.
Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.
Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.
Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.
Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C Quality Level 100, form liquid specific activity 1 units/mL, 10 units/mg protein Торговая марка Calbiochem® storage condition do not freeze foreign activity N-acetylglucosaminidase, - and -galactosidase, -mannosidase, neuraminidase, proteases, none detected shipped in wet ice storage temp. 2-8°C
Safety InformationWGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endoglycoceramidase II from Rhodococcus sp.
Enzyme Commission (EC) Number: 3.2.1.123 ( BRENDA | IUBMB ) MDL number: MFCD03456712
Кат. номер E9030-100MUN E9030-100MUN-PW E9030-100MU ApplicationEndoglycoceramidase II from Rhodococcus sp. has been used in a study to assess the differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography and mass spectrometry. Endoglycoceramidase II from Rhodococcus sp. has also been used in a study to investigate structural and mechanistic analyses of endo-glycoceramidase II.
Unit Definition
One unit will hydrolyze 1 μmol of asialo-GM1 per min at 37 °C at pH 5.0.
Physical form
Solution in 20 mM sodium acetate buffer, pH 6.0, containing 0.2% BSA and 0.1% Lubrol PX.ПараметрыQuality Level 200, recombinant expressed in E. coli form solution mol wt 58.9 kDa shipped in dry ice storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endoglycosidase F1 from Elizabethkingia miricola
Enzyme Commission (EC) Number: 3.2.1.96 ( BRENDA | IUBMB ) MDL number: MFCD00146843 NACRES: NA.32
Кат. номер E9762-0.3UN E9762-1UN E9762-1UN-PW General descriptionEndoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.ApplicationEndoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR) and to glycosylate fucosylated N-glycopeptides.
Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.
Biochem/physiol Actions
Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.
Packaging
Supplied with 5x Reaction Buffer, 250 mM NaH2PO4, pH 5.5.
Unit Definition
One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.
Physical form
Aseptically filled solution in 20 mM Tris-HCl pH 7.5ПараметрыQuality Level 200, form buffered aqueous solution specific activity 16 U/mg mol wt 32 kDa shipped in wet ice storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endoglycosidase F2 from Elizabethkingia miricola
CAS Number: 37278-88-9 Enzyme Commission (EC) Number: 3.2.1.96 ( BRENDA | IUBMB ) MDL number: MFCD00151069 NACRES: NA.32
PackagingКат. номер E0639-.2UN-PW E0639-0.1UN E0639-.2UN E0639-1UN
Supplied with 5x Reaction Buffer, 250 mM sodium acetate, pH 4.5
Unit Definition
One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.
Physical form
Aseptically filled solution in 10 mM sodium acetate, 25 mM sodium chloride, pH 4.5ПараметрыQuality Level 200, recombinant expressed in E. coli form solution specific activity 20 U/mg mol wt 32 kDa shipped in wet ice storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli
Enzyme Commission (EC) Number: 3.2.1.96 ( BRENDA | IUBMB )
Кат. номер 324726-200MIU 324726-200MIUCN General descriptionRecombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185,) and therefore is more suitable for deglycosylation of native proteins. This enzyme is not active above pH 6.0.
Recombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore, is more suitable for deglycosylation of native proteins.
Note: 1 mU = 1 milliunit.
Packaging
200 miu in Plastic ampoule
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.
Physical form
In 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.Other NotesReddy, A., et al. 1998. Glycobiology 8, 633.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C Quality Level 100, form liquid specific activity 20 units/mg protein, 5 units/mL Торговая марка Calbiochem® storage condition do not freeze foreign activity N-acetylglucosaminidase, - and -galactosidase, -mannosidase, neuraminidases, proteases, none detected shipped in wet ice storage temp. 2-8°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endoglycosidase F3 from Elizabethkingia miricola
Enzyme Commission (EC) Number: 3.2.1.96 ( BRENDA | IUBMB ) MDL number: MFCD00146843 NACRES: NA.32
Кат. номер E2264-.2UN E2264-.2UN-PW E2264-0.1UN E2264-.3UN ApplicationEndoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.
Biochem/physiol Actions
Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates -1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.
Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.
Packaging
Supplied with 5x Reaction Buffer, 250 mM sodium acetate, pH 4.5
Unit Definition
One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.
Physical form
Aseptically filled solution in 20 mM Tris-HCl, pH 7.5ПараметрыQuality Level 200, form solution specific activity 30 U/mg mol wt 32 kDa shipped in wet ice storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant, E. coli
Enzyme Commission (EC) Number: 3.2.1.96 ( BRENDA | IUBMB )
Кат. номер 324727-200MIU 324727-200MIUCN General descriptionRecombinant, Elizabethkingia meningosepticum endoglycosidase F3 expressed in E. coli. Cleaves asparagine-linked or free biantennary and triantennary complex, and Man3GlcNAc oligosaccharides from glycoproteins. This enzyme cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine residue. Core fucosylation increases the activity of Endo F3 up to 40 fold. Exhibits no activity on high mannose and hybrid molecules. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.
Recombinant, Elizabethkingia meningosepticum endoglycosidase F3 expressed in E. coli. Cleaves asparagine-linked or free biantennary and triantennary complex, and Man3GlcNAc oligosaccharides from glycoproteins. This enzyme cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine residue. Core fucosylation increases the activity of Endo F3 up to 40 fold. Exhibits no activity on high mannose and hybrid molecules. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185,) and therefore is more suitable for deglycosylation of native proteins.
Packaging
200 miu in Plastic ampoule
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol porcine fibrinogen per min at 37°C, pH 5.5.Other NotesTarentino, A.L., et al. 1995. Glycobiology 5, 599.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C Quality Level 100, form liquid specific activity 30 units/mg protein, 5 units/mL Торговая марка Calbiochem® storage condition do not freeze foreign activity Proteases, none detected shipped in wet ice storage temp. 2-8°C
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endoglycosidase H from Streptomyces plicatus A0810-1UN
CAS Number: 37278-88-9 Enzyme Commission (EC) Number: 3.2.1.96 ( BRENDA | IUBMB ) MDL number: MFCD00146843 NACRES: NA.32 General descriptionEndoglycosidase H or endo--N-acetylglucosaminidase H is an glycohydrolase. It is produced by Streptomyces plicatus and other Streptomyces species.
Biochem/physiol Actions
Endoglycosidase H is involved in cleaving the N-linked glycans present between the two N-acetylglucosamine (GlcNAc) residues in the core of the glycan chain in high-mannose sugars.
Unit Definition
One unit will release N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per min at 37 °C at pH 5.5.
Physical form
Solution in 20 mM Tris HCl, pH 7.5, containing 50 mM NaCl, 1 mM EDTAПараметрыQuality Level 200, recombinant expressed in E. coli form buffered aqueous solution shipped in wet ice storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli
CAS Number: 52769-51-4 Enzyme Commission (EC) Number: 3.2.1.96 ( BRENDA | IUBMB )
Кат. номер 324717-200MIU 324717-200MIUCN General descriptionRecombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
Note: 1 mU = 1 milliunit.
Packaging
200 miu in Plastic ampoule
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.
Physical form
In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.Other NotesRao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C Quality Level 100, form liquid specific activity 3 units/mL, 30 units/mg protein Торговая марка Calbiochem® storage condition do not freeze foreign activity N-acetylglucosaminidase, - and -galactosidase, -mannosidase, neuraminidases, proteases, none detected shipped in wet ice storage temp. 2-8°C
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Enzymatic Protein Deglycosylation Kit
NACRES: NA.32
Кат. номер EDEGLY-1KT EDEGLY-1KT-PW ApplicationEnzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.ПараметрыQuality Level 200 shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS07,GHS08,GHS09 signalword Danger hcodes H302 + H312 - H315 - H317 - H318 - H361fd - H410 pcodes P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313 RIDADR UN 3316 9 WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Glucoamylase, Rhizopus sp.
CAS Number: 9032-08-0 Enzyme Commission (EC) Number: 3.2.1.3 ( BRENDA | IUBMB ) MDL number: MFCD00081350
Кат. номер 172426-5KU 172426-5KUCN General descriptionNative glucoamylase from Rhizopus species. Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. It catalyzes the hydrolysis of 1,4-linked -D-glucose residues in glucosylpolysaccharides, liberating glucose units. Useful for the enzymatic determination of starch.
Native glucoamylase from Rhizopus species. Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. It catalyzes the hydrolysis of 1,4-linked -D-glucose residues in glucosylpolysaccharides, liberating glucose units. Useful for the enzymatic determination of starch.Packaging5 ku in Glass bottleWarningToxicity: Standard Handling (A)Unit DefinitionOne unit is defined as the amount of enzyme that will liberate 1 µmol glucose from maltopentaose per min at 37°C, pH 6.0.ReconstitutionFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.Other NotesHiromi, K., et al. 1973. Biochim. Biophys. Acta302, 362.
Kelly, J.J. and Alpers, D.H. 1973. Biochim. Biophys. Acta315, 113.
Jeffrey, P.L., et al. 1970. Biochemistry9, 1403.
Brown, B. and Brown, D.H. 1965. Biochim. Biophys. Acta110, 124.
Tsujisaka, Y., et al. 1958. Nature181, 770.
Miller, K.D. and Copeland, W.H. 1956. Biochim. Biophys. Acta22, 193.
French, D. and Knapp, D.W. 1950. J. Biol. Chem.187, 463.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions -20C Quality Level 100 form lyophilized specific activity 10 unit/mg solid, 20 units/mg protein Торговая марка Calbiochem® storage condition OK to freeze solubility aqueous buffer: 1 mg/mL, water: 1 mg/mL shipped in wet ice storage temp. 20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P304 + P340 + P312 - P501 WGK Germany WGK 3 Узнать цену -
Glycopeptidase A from almonds
CAS Number: 83534-39-8 Enzyme Commission (EC) Number: 3.5.1.52 ( BRENDA | IUBMB ) MDL number: MFCD00131222 NACRES: NA.32
Кат. номер G0535-.005UN G0535-.005UN-PW General descriptionGlycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine linkages.ApplicationGlycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.
Biochem/physiol Actions
Hydrolyzes an N4-(acetyl--D-glycosaminyl)asparagine in which the N-acetyl-D-glucosamine residue may be further glycosylated, yielding a (substituted) N-acetyl--D-glucoaminylamine and the peptide containing an aspartic residue.
Unit Definition
One unit will hydrolyze 1.0 μmole of ovalbumin glycopeptide per min at pH 5.0 at 37°C.
Physical form
Solution in 50% glycerol containing 50 mM citrate-phosphate buffer, pH 5.0, and BSA.Параметрыform buffered aqueous glycerol solution Quality Level 200, concentration 0.05 unit/mL storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F No data available Flash Point C No data available Узнать цену -
N-Glycosidase F, Elizabethkingia meningosepticum
CAS Number: 83534-39-8 Enzyme Commission (EC) Number: 3.5.1.52 ( BRENDA | IUBMB ) MDL number: MFCD00131222
Кат. номер 362185-100UNU 362185-5000U 362185-100U 362185-100UCN General descriptionNative N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing 1-3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.
Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing 1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.Packaging100 u in Plastic ampouleWarningToxicity: Standard Handling (A)Unit DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.Physical formIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.Other NotesMeans, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыstorage conditions +2C to +8C form liquid Quality Level 100 specific activity 20,000 units/mg protein, 4500 units/mL Торговая марка Calbiochem® storage condition do not freeze foreign activity Proteases, none detected shipped in wet ice storage temp. 2-8°C
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Native Protein Deglycosylation Kit
NACRES: NA.32
Кат. номер NDEGLY-1KT NDEGLY-1KT-PW General descriptionThe Native Protein Deglycosylation is designed for the deglycoslylation of N-linked oligosaccharides from PNGase F-resistant native proteins. Endoglycosidases F1, F2, and F3 are less sensitive to protein conformation than PNGase F and are more suitable for removal of all classes of N-linked oligosaccharides without protein denaturation.
All complex oligosaccharides can be reduced to the trimannosylchitobiose core by treatment of the glycoproteins with neuraminidase, -galactosidase, and N-acetylglucosaminidase. Fucosidases may be required in some situations. The remaining trimannosylchitobiose core structures can be removed with endoglycosidase F3. Biantennary and triantennary structures can be immediately removed by endoglycosidases F2 and F3, respectively. Oligomannose and hybrid structures can be removed by Endoglycosidase F1.
For more information on each type of endoglycosidase, please refer to the Bulletin.ApplicationNative Protein Deglycosylation Kit has been for N-deglycosylation of various enzymes such as laccase, tomato nuclease TBN1 and endo--1,3-glucanase.Storage and StabilityThe NDEGLY Kit ships on wet ice and storage at 2–8 °C is recommended. This kit may be used for at least 1 year when stored as indicated. Several days exposure to ambient temperatures will not reduce the activity of the enzymes in this kit.ПараметрыQuality Level 200 shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
O-Glycosidase from Streptococcus pneumoniae
CAS Number: 9032-92-2 Enzyme Commission (EC) Number: 3.2.1.97 ( BRENDA | IUBMB ) EC Number: 232-888-2 MDL number: MFCD00131003 NACRES: NA.32
Кат. номер G1163-.04UN-PW G1163-.04UN Biochem/physiol ActionsReleases unsubstituted Ser- and Thr-linked -Gal-(13)--GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..PackagingSupplied with 5x Reaction Buffer, 250 mM NaH2PO4 pH 5.0.Unit DefinitionOne unit will hydrolyze 1 µmole of p-nitrophenyl galacto-N-bioside (-Gal-(13)--GalNAc-1C6H4NO2) per min at 37 °C at pH 6.5.Physical formSolution in 50 mM sodium phosphate, pH 7.5Analysis NoteScreened for presence of: β-galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases. See Certificate of Analysis for lot specific information.ПараметрыQuality Level 200 recombinant expressed in E. coli form buffered aqueous solution mol wt 180 kDa concentration 800 units/mL shipped in wet ice storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PNGase F from Elizabethkingia meningoseptica
CAS Number: 83534-39-8 Enzyme Commission (EC) Number: 3.5.1.52 ( BRENDA | IUBMB ) MDL number: MFCD00131222 NACRES: NA.32
Кат. номер P7367-50UN P7367-PH P7367-10UN P7367-300UN P7367-50UN-KC P7367-10UN-PW P7367-300UN-PW P7367-50UN-PW P7367-1VL P7367-25UN General descriptionProteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.ApplicationSuitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis
Used to deglycosylate protein.Biochem/physiol ActionsCleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.Unit DefinitionOne unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.ПараметрыQuality Level 200 grade for proteomics product line BioReagent assay 95% (SDS-PAGE) form powder shelf life 1 weeks at 28 °C (for a reconstituted solution >500 units/ml), 1 yr at 28 °C, Solution is stable for at least 3 freeze-thaw cycles mol wt ~36 kDa concentration 300 units/mL, 50 units/mL optimum pH ~8.6 shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS08,GHS08 signalword Danger-Danger hcodes H334 - H334 pcodes P261 - P284 - P261 - P284 - P304 + P340 + P312 - P501 - P501 Personal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
PNGase F from Elizabethkingia miricola
CAS Number: 83534-39-8 Enzyme Commission (EC) Number: 3.5.1.52 ( BRENDA | IUBMB ) MDL number: MFCD00131222 NACRES: NA.32
Кат. номер G5166-100UN G5166-50UN-PW G5166-100UN-PW G5166-50UN General descriptionPNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.ApplicationPNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.
Used to deglycosylate protein.Biochem/physiol ActionsCleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.Unit DefinitionOne unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.Physical formSupplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTAПараметрыform buffered aqueous solution Quality Level 200 specific activity 20000 units/mg protein mol wt 36 kDa shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P501 Personal Protective Equipment Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
SPAM1 human
NACRES: NA.32
Кат. номер SRP6272-50UG SRP6272-10UG General descriptionHyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1→4)-linkages between N – acetyl – β – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Physical form
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.0-7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.Параметрыbiological source human recombinant expressed in HEK 293 cells assay 92% (SDS-PAGE) form lyophilized powder mol wt calculated mol wt 52.1 kDa","observed mol wt 64-66 kDa (DTT-reduced. Protein migrates due to different glycosylation. Leu 36 is the predicted N-terminus.) packaging pkg of 10 µg","pkg of 50 µg impurities <1 EU/µg endotoxin (LAL test) conjugate 6-His tagged (C-terminus) UniProt accession no. P38567, shipped in wet ice storage temp. 20°C Gene Information human ... SPAM1(6677)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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