FLAG® Purification Sigma-Aldrich
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3X FLAG® Peptide
Empirical Formula (Hill Notation): C120H169N31O49S Molecular Weight: 2861.87 MDL number: MFCD01863911 NACRES: NA.32
Кат. номер F4799-1MG-PW F4799-25MG-PW F4799-4MG-PW F4799-4MG F4799-PH F4799-1MG F4799-25MG F4799-1MG-KC General descriptionThe 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).ApplicationFor use in competitive elution of 3X FLAG® fusion proteins from the ANTI-FLAG® M2 monoclonal antibody (F1804) in solution or bound to agarose on the ANTI-FLAG® M2 agarose affinity gel (A2220). This is achieved by Affinity Chromatography. For the gentle elution of 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gels. 1X FLAG Peptide (F3290) will not elute 3X FLAG fusion proteins from ANTI-FLAG® affinity gels.
Browse additional application references in our FLAG® Literature portal.Preparation NoteTo prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыform lyophilized powder Quality Level 200 concentration (Recommended working concentration is 100 µg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.) shipped in wet ice storage temp. 2-8°C
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Amino-terminal FLAG-BAP™ Fusion Protein
MDL number: MFCD00130921 NACRES: NA.32
Кат. номер P7582-50UG-KC P7582-.1MG-KC P7582-25UG-KC P7582-40UG-KC P7582-50UG P7582-100UG P7582-25UG P7582-40UG P7582-.1MG General descriptionThe Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.
Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 4555kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.ApplicationBrowse additional application references in our FLAG® Literature, portal.
Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.Other NotesControl proteinLegal InformationFLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, form (Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.)mol wt ~49 kDa shipped in dry ice storage temp. 20°C
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Anti-FLAG Tag antibody produced in rabbit SAB4301135-100UL
NACRES: NA.43 General descriptionThe FLAG epitope is composed of eight amino acids with the following sequence AspTyrLysAspAspAspAspLys. It helps in immunoaffinity purification of fusion proteins.SpecificityThe antibody detects transfected proteins contanining FLAG tag.ImmunogenSynthetic peptide: DYKDDDDK conjugated to KLHApplicationApplications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Anti-FLAG Tag antibody produced in rabbit has been used in:- western blotting
- co-immunoprecipitation
- immunofluorescence studies
Features and BenefitsEvaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.Physical formRabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100 antibody form affinity isolated antibody antibody product type primary antibodies clone polyclonal form buffered aqueous solution species reactivity all concentration 1.4 mg/mL application(s) western blot: 1:1000-1:5000 (Cell Lysate) isotype IgG immunogen sequence DYKDDDDK conjugated to KLH (synthetic peptide) conjugate unconjugated shipped in wet ice storage temp. −20°C
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ANTI-FLAG® antibody, Rat monoclonal SAB4200071-200UL
MDL number: MFCD02262912 NACRES: NA.32 General descriptionMonoclonal Anti-FLAG® (rat IgG1 isotype) is derived from the hybridoma 6F7 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG® peptide. The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.
Monoclonal Anti-FLAG® recognizes N-terminal,
C-terminal and internal Flag-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG®-tagged fusion proteins.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in associationith other tags. The small size of the FLAG® tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function.
The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage of the C-terminal FLAG® peptide from a fusion protein catalyzed by Cu2+ ions has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG peptide is found in both rat and mouse Mg2+dependent protein b-phosphatase, as well as in the human and bovine enzyme.ImmunogenFLAG peptide
DYKDDDDKApplicationANTI-FLAG® antibody, Rat monoclonal has been used in:- chromatin immunoprecipitation (ChIP)
- western blotting
- coimmunoprecipitation
- flow cytometric analysis
Browse additional application references in our FLAG® Literature portal.Physical formSolution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыbiological source rat antibody form purified from hybridoma cell culture, purified immunoglobulin antibody product type primary antibodies clone 6F7, monoclonal form buffered aqueous solution species reactivity all application(s) immunoprecipitation (IP): 2.5-5.0 µg using lysates of transiently transfected cells expressing C-terminal-FLAG-tagged protein, western blot: 0.5-1.0 µg/mL using extracts of transiently transfected cells expressing C-terminal-FLAG-tagged protein isotype IgG1 immunogen sequence (DYKDDDDK) conjugate unconjugated shipped in dry ice storage temp. 20°C
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ANTI-FLAG® M2 antibody, Bioreactor grade, Mouse monoclonal
Кат. номер B3111-1MG B3111-0.2MG General descriptionMonoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.
form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mLApplicationIB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.
Packaging
polypropylene screw cap vial
Preparation Note
Dilute the antibody solution from 0.5-10 ug/mL in specified bufferLegal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыbiological source mouse antibody form purified immunoglobulin (purified IgG1 subclass) clone M2, monoclonal shelf life 4 yr purified by using Protein A storage temp. 20°C
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Carboxy-terminal FLAG-BAP™ Fusion Protein
MDL number: MFCD00130921 NACRES: NA.32
Кат. номер P7457-.1MG-KC P7457-.1MG General descriptionCarboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).ApplicationCarboxy-terminal FLAG-BAP™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).Biochem/physiol ActionsThe FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.Other NotesControl proteinPreparation NoteDilute the ANTI-FLAG M2 antibody solution to 10 mg/mlLegal InformationFLAG-BAP is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 form (Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2) mol wt ~49 kDa shipped in dry ice storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-FLAG-Peroxidase antibody produced in rat SAB4200119-200UL
NACRES: NA.32 General descriptionThe product recognizes N-terminal, C-terminal, and internal FLAG-tagged fusion proteins. It is especially recommended for identifying C-terminal FLAG-tagged fusion proteins.
Monoclonal Anti-FLAG-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP). The hybridoma 6F7 was produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG peptide.Immunogenpurified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP).ApplicationBrowse additional application references in our FLAG® Literature, portal.
Monoclonal Anti-FLAG-Peroxidase, recognizes N-terminal, C-terminal and internal FLAG-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG-tagged fusion proteins. The product can be used for immunoblotting.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% merthiolate.Legal InformationFLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыbiological source rat Quality Level 200, antibody form purified immunoglobulin antibody product type primary antibodies clone 6F7, monoclonal form buffered aqueous solution species reactivity all concentration 2-4 mg/mL application(s) western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein isotype IgG1 immunogen sequence DYKDDDDK conjugate peroxidase conjugate shipped in dry ice storage temp. 20°C
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Monoclonal ANTI-FLAG® antibody produced in rabbit
NACRES: NA.32
Кат. номер F2555-100UL F2555-200UL General descriptionEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal FLAG® peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG® peptide is found in both rat and mouse Mg2+ dependent protein b-phosphatase, as well as in the human and bovine enzyme. This rabbit ANTI-FLAG® antibody is produced from the hybridoma SIG1-25. The hybridoma is derived by the fusion of myeloma cells and splenocytes from rabbits immunized with the FLAG® peptide sequence conjugated to KLH.SpecificityThis monoclonal antibody recognizes the FLAG sequence at the N-terminusImmunogenFLAG peptide sequence DYKDDDDKApplicationMonoclonal ANTI-FLAG® antibody produced in rabbit has been used in:- immunofluorescence
- western blot analysis
- immunocytochemistry
- indirect ELISA
Browse additional application references in our FLAG® Literature portal.Physical formProvided as ascites fluid containing 15 mM sodium azide as a preservative.Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of DowПараметрыbiological source rabbit Quality Level 200 antibody form ascites fluid antibody product type primary antibodies clone SIG1-25, monoclonal application(s) immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100, indirect ELISA: suitable, western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein isotype IgG immunogen sequence DYKDDDDK conjugate unconjugated shipped in dry ice storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal ANTI-FLAG® BioM2 antibody produced in mouse
Кат. номер F9291-.2MG F9291-5X1MG F9291-1MG F9291-.2MG-KC General descriptionThe monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins.ApplicationBiotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.
Browse additional application references in our FLAG® Literature portal.Physical formSolution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azidePreparation NoteDilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone M2, monoclonal form buffered aqueous glycerol solution species reactivity all concentration ~1 mg/mL application(s) dot blot: suitable (chemiluminescent detection) isotype IgG1 immunogen sequence DYKDDDDK conjugate biotin conjugate shipped in dry ice storage temp. 20°C
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Monoclonal ANTI-FLAG® M1 antibody produced in mouse
MDL number: MFCD00212941 NACRES: NA.32
Кат. номер F3040-.2MG F3040-.2MG-KC F3040-5MG F3040-1MG General descriptionThe ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.
Method of purification - Protein AImmunogenFLAG; peptide sequence DYKDDDDKApplicationMonoclonal ANTI-FLAG® M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Browse additional application references in our FLAG® Literature portal.Biochem/physiol ActionsBinds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.Physical formSolution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).Preparation NoteDilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.Other NotesM1 antibody binding is calcium dependent.Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone M1, monoclonal form buffered aqueous solution purified by using Protein A species reactivity all concentration 2-5 mg/mL application(s) western blot: 10 µg/mL immunogen sequence DYKDDDDK conjugate unconjugated shipped in dry ice storage temp. 20°C
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Monoclonal ANTI-FLAG® M2 antibody produced in mouse
NACRES: NA.32
Кат. номер F1804-50UG F1804-200UG F1804-5MG F1804-1MG F1804-SAMPLE General descriptionThe ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
Method of purification - Affinity tag purificationSpecificityBinding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-CImmunogenFLAG; peptide sequence DYKDDDDKApplicationFor highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Western Blotting and EIA
Browse additional application references in our FLAG® Literature portal.Physical formContains 50% glycerol in phosphate buffered saline.Preparation NoteImmediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat MilkLegal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 biological source mouse antibody form affinity isolated antibody antibody product type primary antibodies clone M2, monoclonal form buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) purified by affinity chromatography (affinity tag purification) species reactivity all concentration 1 mg/mL isotype IgG1 immunogen sequence DYKDDDDK conjugate unconjugated shipped in wet ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse
NACRES: NA.32
Кат. номер A9469-.2MG A9469-1MG A9469-5X1MG General descriptionMonoclonal ANTI-FLAG® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG® fusion proteins and will recognize the FLAG® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG® peptides).ApplicationMonoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse has been used:- in direct tissue blot immunoassay of sweet orange petioles samples
- in screening internalization of delta opioid receptor
- for screening cell-free protein expression using ELISA
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper),
Physical form
Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservativeLegal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone M2, monoclonal form buffered aqueous glycerol solution species reactivity all concentration ~1 mg/mL application(s) indirect ELISA: 1:20,000 isotype IgG1 immunogen sequence DYKDDDDK conjugate alkaline phosphatase conjugate shipped in wet ice storage temp. 20°C
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Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mouse
NACRES: NA.32
Кат. номер A9594-.2MG A9594-5X1MG A9594-1MG General descriptionMonoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.ApplicationFor simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Browse additional application references in our FLAG® Literature, portal.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper),
Packaging
0.2, 1 mg in poly bottle
Physical form
Solution in phosphate buffered saline plus 1% BSA and preservative
Preparation Note
Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.Other NotesSuggested concentration of 1-10 mg/ml for immunocytochemistry.Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone M2, monoclonal form buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate) species reactivity all concentration ~1 mg/mL application(s) direct immunofluorescence: 10 µg/mL using mammalian cells fixed with methanol:acetone isotype IgG1 immunogen sequence DYKDDDDK conjugate CY3 conjugate shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse
NACRES: NA.32
Кат. номер F4049-.2MG F4049-5X1MG F4049-1MG General descriptionAnti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.ApplicationAntibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity.
Browse additional application references in our FLAG® Literature portal.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)Packaging0.2, 1, 5×1 mg in poly bottlePhysical formSolution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone M2, monoclonal form buffered aqueous solution species reactivity all concentration ~1 mg/mL application(s) direct immunofluorescence: 10 µg/mL using mammalian cells fixed with methanol:acetone isotype IgG1 immunogen sequence DYKDDDDK conjugate FITC conjugate shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse
NACRES: NA.32
Кат. номер A8592-.2MG A8592-5X1MG A8592-1MG General descriptionThe Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides.Packaging0.2, 1 mg in poly bottleApplicationSuggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000
Browse additional application references in our FLAG® Literature portal.
For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.Physical formSolution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizerPreparation NoteDilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl.Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone M2, monoclonal form buffered aqueous glycerol solution species reactivity all concentration ~1 mg/mL application(s) indirect ELISA: 1:20,000 isotype IgG1 immunogen sequence DYKDDDDK conjugate peroxidase conjugate shipped in wet ice storage temp. 20°C
Safety Informationpictograms GHS07,GHS09 signalword Warning hcodes H315 - H317 - H319 - H411 pcodes P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal ANTI-FLAG® M5 antibody produced in mouse
MDL number: MFCD00282246 NACRES: NA.32
Кат. номер F4042-.2MG F4042-.2MG-KC F4042-5MG F4042-1MG General descriptionMonoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.
Method of purification - Protein AImmunogenFLAG; peptide sequence DYKDDDDKApplicationMonoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
Physical form
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)
Preparation Note
Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.Other NotesAntibody is not calcium dependent.Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone M5, monoclonal form buffered aqueous solution purified by using Protein A species reactivity all concentration 2-5 mg/mL application(s) western blot (chemiluminescent): 10 µg/mL isotype IgG1 immunogen sequence DYKDDDDK conjugate unconjugated shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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