Bone Morphogenetic Protein 2 human
MDL number: | MFCD03097877 |
NACRES: | NA.77 |
Кат. номер |
H4791-10UG-PW |
H4791-10UG |
H4791-5UG |
Bone Morphogenetic Protein 2 is a member of the TGF-β superfamily of cytokines that affect bone and cartilage formation. It is important for skeletal development during embryogenesis. BMP-2 induces chondrocyte formation, osteoblast differentiation, and is involved in embryo dorsal-ventral patterning and organogenesis.
Packaging
10 μg in poly bottle
Biochem/physiol Actions
It has been reported that Bone Morphogenetic Protein 2 (BMP-2) inhibits estradiol induced proliferation of human breast cancer cells. BMP-2 signaling mediates apoptosis by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6. Cellular responses to BMP-2 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors, which play significant roles in BMP binding and signaling. One BMP type II receptor and two BMP type I receptors have been identified. Both BMP type I receptors bind BMP-2 with high-affinity in the absence of BMP receptor type II.
Physical form
Lyophilized from a 0.2 μm filtered solution of 2x PBS + 6% Ethanol.
Preparation Note
This Bone Morphogenetic Protein 2 (BMP-2) is produced from a DNA sequence encoding the human BMP-2 protein, expressed in HEK 293 cells. It is a glycosylated homodimer linked by a single disulfide bond with an apparent molecular mass of 30-38 kDa.
Analysis Note
The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (mouse chondrogenic cell line).
HumanKine is a registered trademark of Proteintech Group, Inc.
Quality Level | 100 |
biological source | human |
recombinant | expressed in HEK 293 cells |
assay | >95% (SDS-PAGE) |
form | lyophilized powder |
potency | 60 ng/mL EC50 |
quality | endotoxin tested |
mol wt | dimer 30-38 kDa (glycosylated) |
packaging | pkg of 10 µg |
Торговая марка | Corning |
storage condition | avoid repeated freeze/thaw cycles |
application(s) | cell culture | mammalian: suitable |
impurities | 1 EU/µg Endotoxin level |
UniProt accession no. | P12643, |
storage temp. | 20°C |
Gene Information | human ... BMP2(650) |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |