Bioprocessing Recombinant Supplements Sigma-Aldrich
- Сортировать:
- Вид таблицей
-
Cell Freezing Medium-DMSO Serum free 1x C6295-50ML
NACRES: NA.75 General descriptionCell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.ApplicationCell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.Other NotesComplete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.ПараметрыQuality Level 200 sterility sterile-filtered form liquid application(s) cell culture | mammalian: suitable, cryopreservation: suitable impurities endotoxin, tested shipped in dry ice storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
D-(+)-Glucose solution
Empirical Formula (Hill Notation): C6H12O6 CAS Number: 50-99-7 Molecular Weight: 180.16 Beilstein/REAXYS Number: 1281608 EC Number: 200-075-1 MDL number: MFCD00063774 PubChem Substance ID: 24895351 NACRES: NA.75
Кат. номер G8769-500ML G8769-PH G8769-100ML General descriptionGlucose is the energy currency essential for physiological processes in the living cells. It serves as a precursor for glycoproteins, glycolipids and glycosaminoglycans.ApplicationD-(+)-Glucose solution has been used as a medium supplement:- in Dulbeccos Modified Eagle Medium (DMEM) for culturing Flp-In 293 cells
- in dissection medium for coronal slices from rat brain
- in neurobasal medium for culturing hippocampi cells
Provides the primary energy source for cell metabolism.Biochem/physiol ActionsGlucose is a crucial component for the growth and maintenance of mammalian cells in in vitro conditions and is essential for productivity and quality. Its high metabolic turnover favors osteoclast differentiation. The levels of glucose modulate the migration and growth of tumor cells. HeLa cells display high mobility at low glucose levels.ПараметрыQuality Level 200 sterility sterile-filtered product line BioXtra form solution concentration 45% in H2O application(s) cell culture | mammalian: suitable impurities endotoxin, tested SMILES string OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O InChI 1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6+/m1/s1 InChI key WQZGKKKJIJFFOK-DVKNGEFBSA-N Gene Information human ... PYGM(5837)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Demecolcine solution
CAS Number: 477-30-5 MDL number: MFCD00075459 PubChem Substance ID: 24893569
Кат. номер D1925-10ML D1925-100ML D1925-20ML ApplicationUsed for cell synchronization by arresting them at metaphase. Qualified for use in animal component free applications.Other NotesTandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. D1925.pdf , Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.,Legal InformationColcemid is a trademark of Ciba-Geigy CorporationПараметрыQuality Level 400, sterility sterile-filtered product line BioXtra form liquid concentration 10 µg/mL in HBSS application(s) cell culture | mammalian: suitable storage temp. 2-8°C SMILES string CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13 InChI 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1 InChI key NNJPGOLRFBJNIW-HNNXBMFYSA-N
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Dimethyl sulfoxide
Empirical Formula (Hill Notation): C2H6OS Linear Formula: (CH3)2SO CAS Number: 67-68-5 Molecular Weight: 78.13 Beilstein/REAXYS Number: 506008 EC Number: 200-664-3 MDL number: MFCD00002089 PubChem Substance ID: 24893688 NACRES: NA.75
Кат. номер D2438-50ML D2438-500ML D2438-5X10ML Features and BenefitsReady-to-use sterile filtered product conveniently packaged in septum screw-capped amber bottles. Meets USP and EP testing requirementsCautionSupercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.Параметрыgrade BioPerformance Certified Quality Level 400 Agency/Method meets EP testing specifications, meets USP testing specifications vapor density 2.7 (vs air) vapor pressure 0.42 mmHg ( 20 °C) sterility sterile-filtered assay (USP XXIV) form liquid autoignition temp. 573 °F expl. lim. 42 %, 63 °F application(s) cell culture | hybridoma: suitable, cryopreservation: suitable impurities 0.1% water
watercolor colorless refractive index n20/D 1.479 (lit.) bp 189 °C (lit.) mp 16-19 °C (lit.) solubility H2O: miscible (completely) density 1.10 g/mL (lit.) SMILES string CS(C)=O InChI 1S/C2H6OS/c1-4(2)3/h1-2H3 InChI key IAZDPXIOMUYVGZ-UHFFFAOYSA-N
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type ABEK (EN14387) respirator filter RIDADR NA 1993 / PGIII WGK Germany WGK 1 Flash Point(F) 188.6 °F - closed cup Flash Point(C) 87 °C - closed cup Узнать цену -
Earles Balanced Salts
MDL number: MFCD00217343 NACRES: NA.75
Кат. номер E2888-6X500ML E2888-500ML E2888-100ML ПараметрыQuality Level 400 sterility sterile-filtered form liquid application(s) cell culture | mammalian: suitable impurities endotoxin, tested components NaHCO3: 2.2 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/Lshipped in ambient
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Geys Balanced Salt Solution
NACRES: NA.75
Кат. номер G9779-500ML G9779-6X500ML General descriptionGeys Balanced Salt Solution is a phosphate bicarbonate buffered cell suspension and washing solution. A complete balanced salt solution with a unique combination of ""salt forms"". Contains the same five basic ions as EBSS, HBSS and DPBS.Параметрыsterility sterile-filtered Quality Level 400 form liquid application(s) cell culture | mammalian: suitable impurities endotoxin, tested components NaHCO3: 2.27 g/L
phenol red: no
glucose: 1.0 g/Lshipped in ambient storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
HEPES solution
CAS Number: 7365-45-9 MDL number: MFCD00006158 PubChem Substance ID: 24895414 NACRES: NA.75
Кат. номер H0887-20X100ML H0887-20ML H0887-100ML H0887-PH General descriptionHEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.ApplicationHEPES has been used as a component of:- Hanks balanced salt solution, Dulbeccos modified eagles medium and no-glucose DMEM, which are used for the preparation of tissue slices
- Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity
CautionConcentrations of HEPES over 40mM may be toxic to some cell types.Preparation NotePrepared from HEPES free acid and cell culture grade water.ПараметрыQuality Level 300 sterility sterile-filtered product line BioReagent, BioXtra form liquid concentration 1 M application(s) cell culture | mammalian: suitable impurities endotoxin, tested pH 7.0-7.6 useful pH range 6.8 - 8.2 pH range 7.0 - 7.6 storage temp. 2-8°C SMILES string OCCN1CCN(CCS(=O)(O)=O)CC1 InChI 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) InChI key JKMHFZQWWAIEOD-UHFFFAOYSA-N
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Histopaque®-1077 Hybri-Max™
NACRES: NA.75
Кат. номер H8889-500ML H8889-100ML ApplicationUsed to create a density medium for the purification of lymphocytes and other mononuclear cells. Histopaque®-1077 is a solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell mediated lympholysis and for human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step prior to enumeration of T-, B-, and ‘null lymphocytes.Legal InformationHistopaque is a registered trademark of Sigma-Aldrich Co. LLC
Hybri-Max is a trademark of Sigma-Aldrich Co. LLCПараметрыsterility sterile-filtered Quality Level 400 product line BioReagent form liquid application(s) cell culture | hybridoma: suitable impurities endotoxin, tested density 1.077 g/cm3 at 25 °C (lit.) storage temp. 2-8°C
Safety InformationPictograms GHS08 Signal Word Danger Hazard Statements H317 - H334 - H350 Precautionary Statements P202 - P261 - P272 - P280 - P302 + P352 - P308 + P313 Personal Protective Equipment Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Hydrochloric acid solution
Empirical Formula (Hill Notation): HCl CAS Number: 7647-01-0 Molecular Weight: 36.46 MDL number: MFCD00011324 PubChem Substance ID: 24895872 NACRES: NA.71
Кат. номер H9892-100ML H9892-RM H9892-1L ApplicationHydrochloric acid solution (HCl solution) is mainly used to adjust the pH in cell culture medium.
It can also be used:- In the extraction of elderberry anthocyanins by endothelial cells.
- To convert iron(III) perchlorate to Fe(III) ions which can then form a complex with acetohydroxamic acid (FeAHA).
ПараметрыQuality Level 200 sterility sterile-filtered product line BioReagent form solution concentration 1.0 N application(s) cell culture | mammalian: suitable anion traces chloride (Cl-): passes test SMILES string Cl InChI 1S/ClH/h1H InChI key VEXZGXHMUGYJMC-UHFFFAOYSA-N
Safety InformationPictograms GHS05 Signal Word Warning Hazard Statements H290 Precautionary Statements P234 - P390 RIDADR UN 1789 8 / PGIII WGK Germany WGK 1 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
Krebs-Henseleit Buffer Modified
Кат. номер K3753-25KG K3753-50KG K3753-50K K3753-25K K3753LF1-BIBC K3753-10L K3753-1L K3753-BIBC K3753-10X1L QuantityFormulated to contain 9.6 grams of powder per liter of medium.ReconstitutionSupplement with 2.1 g/L sodium bicarbonate.Параметрыform powder application(s) cell culture | mammalian: suitable storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Lipids Cholesterol Rich from adult bovine serum
NACRES: NA.75
Кат. номер L4646-RM L4646-20ML L4646-100ML L4646-PH L4646-100ML-PW L4646-20ML-PW General descriptionCholesterol is a lipid molecule, found integrated to the cell membrane.ApplicationLipids Cholesterol Rich from adult bovine serum has been used as one of the lipid component to generate erythroblasts ex vivo. It has also been used as a standard to determine muscle cholesterol level, spectrophotometrically.Biochem/physiol ActionsCholesterol significantly affects the property of phospholipid membrane such as fluidity and membrane protein function. It exhibits a condensing effect towards phospholipids. Cholesterol-phospholipid interaction influences physiological properties of a lipid bilayer. Cholesterol serves as a precursor of bile acids, steroid hormones and vitamin D. Uncontrolled level of plasma cholesterol might lead to the risk of cardiovascular disorder.Other NotesAqueous lipoprotein solutionПараметрыsterility sterile-filtered Quality Level 200, product line BioReagent form liquid composition Cholesterol, 9.0-11.0 g/L , Protein, 15.0-25.0 g/L application(s) cell culture | insect: suitable, cell culture | mammalian: suitable impurities Adventitious viral agents, none detected (9 CFR; 113.53), IgG, none detected (RID), Microbial content, none detected (USP XXII), Mycoplasma, none detected (Barile method), ≤6.0 EU/mg cholesterol endotoxin storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
MEM Amino Acids (50x) solution
MDL number: MFCD00240873 eCl@ss: 32160406 NACRES: NA.75
Кат. номер M5550-100ML M5550-1L M5550-500ML ApplicationAdding supplements of amino acids to media stimulates growth and prolongs the viability of cells in culture. MEM Amino Acids (50x) solution concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro. It has been used in in vitro cultures, co-cultures and incubation media.Storage and StabilityThis product may contain a yellow hue if exposed to light. This is due to the presence of the amino acids tryosine and tryptophan in the solution. The change in color to the liquid solution has no affect to the quality or integrity of the product. For a complete list of components, please see the formulation sheet listed in the "Documents" section of the product page.ПараметрыQuality Level 200 sterility sterile-filtered product line BioReagent form liquid application(s) cell culture | mammalian: suitable impurities endotoxin, tested storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Узнать цену -
RPMI 1640 Vitamins Solution (100)
NACRES: NA.75
Кат. номер R7256-100ML R7256-PH R7256-500ML General descriptionRoswell Park Memorial Institute medium is commonly referred as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. RPMI 1640 is used for the serum-free expansion of human lymphoid cells.ApplicationRPMI 1640 Vitamins Solution (100x) is used as a supplement in cell culture media.Параметрыsterility sterile-filtered Quality Level 200, product line BioReagent form liquid application(s) cell culture | mammalian: suitable impurities endotoxin, tested color yellow to dark yellow pH 7.2-7.6 shipped in dry ice storage temp. −20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Serum Plus™ Medium Supplement
Кат. номер 14008C-100ML 14008C-PH 14008C-500ML General descriptionSERUM PLUS™ contains a low level of gamma irradiated Fetal Bovine Serum (FBS) supplemented with proteins that promote nutrient transport, cell attachement, and cell growth.ApplicationSerum Plus™ is a medium supplement that can be used as a serum substitute for most cell culture applications. It has been used as a supplement in media for maintenance of bloodstream form Trypanosoma brucei Lister 427.Features and BenefitsBenefits include less than half of the total protein concentration found in serum, reduced levels of inhibitors such as proteases and viral antibodies, and consistency of lot-to-lot performance due to greater control of the component materials.Preparation NoteThawing
1. Remove the supplement bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
2. Place each container in a 30 - 37 °C water bath or incubator. Excessive temperatures will degrade heat liable nutrients. If using a water bath, prevent the bottle caps from being completely submerged.
3. Gently swirl or shake the bottles every 10-15 minutes until the serum is completely thawed.
4. After thawing, use the supplement promptly. Liquid supplement may be stored refrigerated (2 - 8 °C) up to four weeks. To avoid freeze/thaw cycles or long periods of refrigeration, it is recommended that any unused supplement be immediately dispensed into small, useful aliquots and refrozen for future use.
Note:
Periodic agitation is crucial for optimum performance. If a bottle is not periodically shaken or swirrled as it thaws, gradients containing high concentrations of salts, proteins and lipids will form throughout the liquid portion and lead to the formation of crystalline or flocculent precipitates. These cryoprecipitates are not toxic to cell cultures, but they affect the appearance and consistency of each bottle.
Small amounts of cryoprecipitates are not uncommon, and will not affect product performance. Gently warming and mixing the supplement will generally allow the material to go back into solution.Legal InformationSER-TAIN is a trademark of Sigma-Aldrich Co. LLC
SERUM PLUS is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 500, sterility -irradiated by SER-TAIN™ process (FBS), sterile; sterile-filtered shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sodium chloride solution
Empirical Formula (Hill Notation): ClNa Linear Formula: NaCl CAS Number: 7647-14-5 Molecular Weight: 58.44 Beilstein/REAXYS Number: 3534976 MDL number: MFCD00003477 PubChem Substance ID: 24899816 NACRES: NA.75
Кат. номер S8776-1L S8776-20ML S8776-10X10ML S8776-100ML S8776-6X20ML ApplicationSodium chloride is widely used in biochemistry and molecular biology research. It is a component of phosphate buffered saline (Product No. P3813) and βC buffer (Product Nos. S0902 and S8015). Sodium chloride is a component of all media formulations. This product has been qualified for use in preparation of in vitro solutions.PackagingPET bottle (100 ml), PETG bottle (10 ml), with capsPreparation NotePrepared in tissue culture grade water.ПараметрыQuality Level 200 sterility sterile-filtered product line BioXtra concentration 0.9% in water application(s) cell culture | mammalian: suitable impurities endotoxin, tested SMILES string [Na+].[Cl-] InChI 1S/ClH.Na/h1H;/q;+1/p-1 InChI key FAPWRFPIFSIZLT-UHFFFAOYSA-M
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену -
TrypZean® Solution, 1
NACRES: NA.75
Кат. номер T3449-500ML T3449-100ML ApplicationUsed to release adherent cells from tissue culture plates for passaging. Eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.Legal InformationTrypzean is a registered trademark of ProdiGene, Inc.ПараметрыQuality Level 200, recombinant expressed in corn sterility sterile-filtered form aqueous solution concentration 1 x application(s) cell culture | mammalian: suitable pH 7.70-8.30 shipped in dry ice storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Tyrodes Salts
Кат. номер T2145-25KG T2145-50KG T2145-25K T2145-10L T2145-BIBC T2145-10X1L T2145-100L T2145-50K T2145LF1-BIBC T2145-1L General descriptionTyrode′s Salts play an important role in tissue culture. A salt solution′s basic function is to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.ApplicationTyrodes Salts has been used:- for seeding cardiomyocytes culture for optical based phenotyping and imaging studies
- to incubate 3T3-L1 pre-adipocytes in calcium intracellular assays
- as a medium for the isolation and culturing of myofiber extensor digitorum longus (EDL) muscle
QuantityFormulated to contain 9.6 grams of powder per liter of medium.ReconstitutionSupplement with 1.0 g/L sodium bicarbonate.Параметрыform powder application(s) cell culture | mammalian: suitable storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа
Анна Гловацкая
Менеджер по работе с клиентами
С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
