Universal Protease Substrate 11734334001

The Universal Protease Substrate provides rapid and highly sensitive detection of trace protease activity, or determination of the effectiveness of protease inhibition. Protease activity releases resorufin-labeled peptides from the patented substrate, resorufin-labeled casein. Resorufin is N-(resorufin-4-carbonyl)-piperidine-4-carbonic acid).The concentration of these labeled peptides in the supernatant is directly related to the proteolytic activity present.

• Conveniently detect nanogram quantities of proteolytic activity in less than one hour.
• Perform highly sensitive protease detection in a homogeneous assay.

Principle
By treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present.
Use Universal Protease Substrate as a general substrate for proteases, and to detect trace protease activities, for example, as a contamination in enzyme preparations. It can be measured spectrophotometrically and fluorimetrically in a homogeneous assay.
Quality
Function test: performance-controlled in the assay for proteases.
Preparation Note
Working concentration: Approximately 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
An aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 °C the product is rapidly hydrolyzed in solution.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
packaging	pkg of 40 mg (40 μmol; 100mM)
Торговая марка	Roche
concentration	1 mg/mL
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable