Trypsin Sequencing Grade, modified

Enzyme Commission (EC) Number:

3.4.21.4 ( BRENDA | IUBMB )

Кат. номер

11418033001

11418025001

Trypsin Sequencing Grade, modified, is isolated from bovine pancreas as a highly purified and specific protease, and subsequently modified.

Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, 2-macroglobulin,1-antitrypsin, APMSF, and antipain.
Trypsin is a serine endopeptidase. At pH 7.5–9, it specifically hydrolyzes proteins and peptide bonds C-terminally of Iysine and arginine. Amide and ester bonds of Arg and Lys are also cleaved. The specificity of Trypsin Sequencing Grade, modified, is verified with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Trypsin Sequencing Grade, modified, one part by weight enzyme with 9 parts by weight insulin Box, are incubated for 18 hours to detect traces ofchymotrypsin impurities.
Use Trypsin Sequencing Grade, modified, to generate glycopeptides from purified glycoproteins.
It is used for:

• Protein-structure elucidation
• Tryptic mapping
• Fingerprinting analysis
• Sequence analysis
• Translocation studies
• Protein identification

Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC.
Working concentration: 1/100 to 1/5 of the protein by weight
Storage conditions (working solution): -15 to -25 °C
Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.
A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100
form	lyophilized (salt-free)
packaging	pkg of 4 x 100 μg (11418033001), pkg of 4 x 25 μg (11418025001)
Торговая марка	Roche
optimum pH	8.0
shipped in	wet ice
storage temp.	2-8°C

RIDADR

NONH for all modes of transport