Transforming Growth Factor-1, human (hTGF-1) 11412272001

Recombinant, human TGF- 1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 µm pore size membrane.
Specificity
Species specificity: Transforming growth factor-1 (TGF-1) is effective on human and mouse cells.
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.
Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.
Biochem/physiol Actions
Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.
Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.
Quality
Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/µg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)
Sequence
Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.
Chain Length 2 x 112 AA
Unit Definition
EC50 definition: The concentration of hTGF-1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.
Preparation Note
Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF- 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-1 solution (1 µg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human
recombinant	expressed in CHO cells
assay	>95% (SDS-PAGE)
form	solution
potency	<0.1 ng/mL EC50
mol wt	25,000 Da
packaging	pkg of 1 mL (1 µg)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
impurities	<0.1 EU/µgtested (LAL test)
UniProt accession no.	P01137,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... TGFB1(7040)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash