Titan One Tube RT-PCR Kit 11939823001

Titan™ One Tube RT-PCR Kit uses Avian Myeloblastosis Virus (AMV) reverse transcriptase for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA polymerase and Tgo DNA polymerase for amplification of cDNA by PCR. Tgo DNA polymerase possesses proofreading activity leading to an increased PCR fidelity. This kit reduces the error rate, allows the amplification of fragments up to 6 kb by RT-PCR, delivers increased sensitivity. It is considered faster than conventional two-step RT-PCR.
The Titan One Tube RT-PCR Kit is designed for fast, sensitive and reproducible analysis of RNA by high fidelity reverse transcription polymerase chain reaction (RT-PCR) in a one-step reaction.
Specificity
Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV
Titan One Tube RT-PCR Kit has been used:

• to complete cDNA synthesis in reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
• to clone sirtuin 6 (SIRT6) into the backbone of pDsRed2-N1
• to amplify full-length and truncated cDNA fragments of C-X-C motif chemokine 5 (CXCL5)
• to analyze RNA (total RNA, poly (A)+ RNA, viral RNA) from a variety of sources (animal, plant, human, viral, and bacterial). In a single tube, the Titan One Tube RT-PCR Kit will reverse transcribe RNA into cDNA,† then amplify the cDNA with a thermal cycling reaction. The enzymes AMV Reverse Transcriptase and the Expand High Fidelity PCR System are used, respectively. Depending on the primers chosen, a specific message or the entire population of transcripts can be amplified. The RT-PCR method also permits the cloning of rare messages without the need to construct cDNA libraries. The kit may be used for:Qualitative, semi quantitative, or quantitative analysis of RNA transcription levels even in single cells in combination with gel-based detection methods or ELISA-based detection methods
• for cloning of RNA up to 6 kb
• for mutation analysis at RNA level in combination with sequencing or other mutation scanning techniques

Features and Benefits

• Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparison to Taq DNA polymerase.
• Delivers increased sensitivity due to the high efficiency of all three enzymes.

Is faster than conventional two-step RT-PCR.
Packaging
1 kit containing 9 components
For life science research only. Not for use in diagnostic procedures.
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
Expand is a trademark of Roche
Titan is a trademark of Roche

Quality Level	100,
usage	sufficient for 50 reactions
feature	dNTPs included: no, hotstart: no
Торговая марка	Roche
parameter	42-60 °C optimum reaction temp.
application(s)	RT-PCR: suitable
input	purified RNA
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H319 - H412
pcodes	P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash