T4 DNA Polymerase

Enzyme Commission (EC) Number:

2.7.7.7 ( BRENDA | IUBMB )

Кат. номер

11004786001

11004794001

T4 DNA Polymerase is a DNA-dependent DNA polymerase that catalyzes the addition of deoxynucleoside-5-triphosphates to the hydroxyl termini of recessive ends.
The enzyme has a very active 35 exonuclease with a high specificity for single-stranded DNA; it lacks 53 exonuclease activity.
T4 DNA Polymerase is a DNA-dependent DNA polymerase that catalyzes the addition of deoxynucleoside-5-triphosphates to the hydroxyl termini of recessive ends.
The enzyme has a very active 35 exonuclease with a high specificity for single-stranded DNA; it lacks 53 exonuclease activity.
Contents

• Enzyme Solution, 1 U/µl, in buffer, pH 8.0
• Incubation Buffer, 5x concentrated

• Sample Materials: DNA with 5-protruding ends

Site-directed mutagenesis: Together with T4 gene 32 protein, T4 DNA polymerase can fill in single-stranded gaps during site-directed mutagenesis experiments.

• Superiority of hybridization probes prepared with T4 DNA polymerase: Polymerase-labeled probes have two advantages over probes prepared by nick translation:

a) They do not have artificial hairpin structures.
b) They can easily be converted into strand-specific probes by restriction endonuclease cleavage.
T4 DNA polymerase is used to label 3-termini of DNA. Extensive labeling is achieved by the replacement reaction, in which the 3-exonuclease activity of the enzyme first digests dsDNA to produce molecules with recessed 3-termini. On subsequent addition of labeled dNTPs, the polymerase activity of T4 DNA polymerase then extends the 3 ends along the length of the template. Exonuclease III from E. coli can be used to create partially single-stranded dsDNA for subsequent polymerization reactions. Molecules labeled to high specific activity are used primarily as hybridization probes. They have two advantages over probes prepared by nick translation: they lack artificial hairpin structures and they can easily be converted into strand-specific probes by cleavage with suitable restriction endonucleases. In combination with T4 Gene 32 Protein, T4 DNA polymerase is used for gap-filling in site-directed mutagenesis experiments.
Packaging
1 kit containing 2 components
Quality
Absence of endonucleases, ss-specific DNases, nicking activity, and RNases tested according to the current Quality Control procedures.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 100 U (11004786001), pkg of 500 U (11004794001)
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

RIDADR

NONH for all modes of transport