Roche® Life Science Products Roche

3.2.1.1 ( BRENDA | IUBMB )

α-Amylase or 1,4-α-D-glucan-glucanohydrolase is a family of starch converting endoamylase enzymes. Endoamylases cleave at the endo or internal α,1-4 glycosidic bonds present in amylose and amylopectin. This reaction results in variable length oligosaccharides such as α-dextrins, which are branched oligosaccharides.
Specificity
Hydrolyzes α1,4-glucan bonds in polysaccharides with three or more α1,4-bound glucose units.

-amylase is used for the hydrolyzation of 1,4-glycosidic bonds in polysaccharides containing 3 or more 1,4-linked D-Glucose units. The enzyme produces -dextrins.
Packaging
50mg (5 mL)
Unit Definition
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 µCi (32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Preparation Note
Activator: NaCI (10 mM) activates the pancreatic enzyme.
Stabilizers: Both, the bacterial and the pancreatic enzymes are stabilized by Ca2+ (bound Ca2+ is required for activity).
Working concentration: 2.5 to 5 mg/ml
Working solution: Phosphate buffer (100 mmol/l; pH 7.0).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~1000 units/mg protein (At 25 °C with soluble starch as the substrate, standardized with BSA.)
mol wt	M_r 50 kDa
packaging	pkg of 5 mL
Торговая марка	Roche
optimum pH	7
shipped in	wet ice

pictograms	GHS08
signalword	Danger
hcodes	H334
pcodes	P261 - P284 - P304 + P340 - P342 + P311 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

-Gal Reporter Gene Assay, chemiluminescent 11758241001

The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid.

The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene.
Features and Benefits

Sensitive: Chemiluminescence technology allows detection of approximately 20 fg -galactosidase in cell extracts
High dynamic measuring range: Linear range over four to five orders of magnitude
Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
Safe: No radioactive isotopes are used
Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control

Packaging
1 kit containing 7 components.
Preparation Note
Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 250 assays (tubes), sufficient for 500 assays (microplate)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS02,GHS05,GHS07
signalword	Danger
hcodes	H225 - H290 - H314 - H317 - H336 - H412
pcodes	P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378
Target Organs	Central nervous system
RIDADR	UN 3316 9
WGK Germany	WGK 2

Узнать цену

Подробнее

-Gal Staining Set 11828673001

The -Gal Staining Set detects bacterial -Gal activity for direct visualization in transfected cells. Forms a blue precipitate and simplifies the histochemical staining of cells and tissue sections for light microscopy. Upon transfecting cells with a -Gal construct (encoded by the lacZ gene), cells are fixed, washed in PBS (phosphate buffered saline) and stained with freshly prepared staining solution. After staining, cells are washed again in PBS and evaluated by microscopy. When long-term storage is desired, PBS is replaced by mounting media (e.g., glycerol).
For immunohistochemical staining of cells or tissue sections, incubate the specimen with an antibody conjugated to bacterial -galactosidase, according to standard protocols. Subsequently, the specimen is processed for detection of -Gal as described for transfected cells.
Specificity
Bacterial β-galactosidase

The β-Gal Staining Set is used for the histochemical staining of cells and tissue sections demonstrating β-galactosidase activity.
β-Gal staining set has been used in immunofluorescence analysis, senescence assay and histopathologic analysis of tissue samples.
Packaging
1 set containing 2 components.
Specifications
Stability: Stable at +2 to +8°C

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
usage	sufficient for 100 tests (in 3.5 cm dishes)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

-Galactose Dehydrogenase

1.1.1.48 ( BRENDA | IUBMB )

D-galactose:NAD+ 1-oxidoreductase
Specificity
Galactose dehydrogenase will oxidize D-galactose [Km = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32).
Quality
Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH
Sequence
Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D).
Unit Definition
One unit galactose dehydrogenase will oxidize 1 mol of -D-galactose in one minute at +25 °C and pH 8.6.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.
Physical form
Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.)
packaging	pkg of 1 mL (10104973001 [1 mg]), pkg of 1 mL (10104981001 [5 mg])
Торговая марка	Roche
optimum pH	8.4 (0.1 M in Tris buffer)
pH range	4.6 - 9.2
shipped in	wet ice
storage temp.	2-8°C

Узнать цену

Подробнее

-Galactose Dehydrogenase S 10662046001

1.1.1.48 ( BRENDA | IUBMB )

D-galactose:NAD+ 1-oxidoreductase

β-Galactose Dehydrogenase S has been used in the colorimetric microassay method to determine the level of galactose and galactose-1-phosphate in blood.
Quality
Contaminants: <0.01% ADH, <0.01% -galactosidase, <0.1% LDH, <0.05% NADH oxidase
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	80 U/mg (Approximately 80 U/mg protein at +25°C with galactose as the substrate.), ~80 units/mg protein (At 25 °C with galactose as the substrate.)
packaging	pkg of 50 U
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

-Galactosidase 10105031001

3.2.1.23 ( BRENDA | IUBMB )

-Galactosidase or -D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains, each constituting 1023 amino acids. It is very specific for D-galactose and requires K+ or Na+ and Mg2+ to be fully active. -Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with -d-galactopyranosides.
Specificity
Cleaves terminal galactose residues that are β1,4-linked to a monosaccharide, oligosaccharide, or glycopeptide.

β-galactosidase has been used in enzyme-linked immunosorbent assay (ELISA). Use β-Galactosidase to produce a calibration curve in enzymatic assays.
Quality
Contaminants: <0.01% GIDH, GPT, LDH, MDH, and oxaloacetate decarboxylase, each
Physical form
Suspension, in 3.2 M ammonium sulfate solution, pH approximately 6, crystalline
Preparation Note
The ammonium sulfate preparation is stable at 2 to 8 °C until the expiration date printed on the label. Use directly for most applications, e.g., quantitation of lactose.
In the absence of ammonium sulfate, solutions of -galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM -mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.
Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl--D-galactopyranoside.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~300 units/mg protein (At 37 °C with 2-nitrophenyl--D-galactoside as the substrate, approximately 30 U/mg at 25 °C with lactose as the substrate; standardized with BSA.)
mol wt	540 kDa
packaging	pkg of 1,500 U
Торговая марка	Roche
optimum pH	7
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

Узнать цену

Подробнее

2-Macroglobulin 10602442001

α2-Macroglobulin belongs to the macroglobulin superfamily. It is a high molecular weight tetrameric glycoprotein with receptor-binding domain (RBD), thioester-containing domain (TED) domain and C1r/C1s, Uegf, Bmp1 (CUB) domain.

α2-Macroglobulin has been used as a component of homogenization buffer for processing hemibrains of young mice.
Biochem/physiol Actions
α2-Macroglobulin is a plasma endopeptidase and glycoprotein which functions on proteinases, especially serine proteases. It interacts with and inhibits endopeptidases. However, it does not act on other proteases, including inactive proteinases.
α2-Macroglobulin (α2M) binds several hormones and may regulate their activity. α2M also aids protection against various infections.
Sequence
2-macroglobulin, a glycoprotein, is a tetramer of identical subunits, which are Iinked in pairs by disulfide bonds.
Preparation Note
Working solution: Recommended solvent is distilled water.
Storage conditions (working solution): -15 to -25 °C
Reconstitution
Soluble in water. Stable for at least one week at room temperature (15 to 25 °C), or 3 weeks at 2 to 8 °C. Can also be frozen in aliquots at -15 to -25 °C, where it remains stable for at least 6 months. Sensitive to acidic pH, denatured below pH 4.0. Ammonia methylamine and hydroxylamine (above pH 7.0) cause irreversible conversion to the inactive form.
Note: Do not use 2-macroglobulin in the presence of DTT since it causes a (reversible) dissociation into inactive subunits. 2-macroglobulin acts by physically entrapping endoproteinases, usually in a 1:1 ratio.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)
Analysis Note
Specific activity: approximately 1 inhibitor U/mg protein (tested with trypsin at +25°C and Chromozym TRY as substrate).
Crossreactivity with pregnancy associated glycoproteins: deglycosylation of 2-macroglobulin is recommended.

For life science research only. Not for use in diagnostic procedures.

biological source	bovine plasma
Quality Level	100
form	lyophilized (Contains 6% BSA)
packaging	pkg of 25 IU
Торговая марка	Roche
application(s)	blocking: suitable
pH range	4 - 7
UniProt accession no.	Q7SIH1,
shipped in	wet ice
Gene Information	bovine ... A2M(513856)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

3-Hydroxybutyrate Dehydrogenase (3-HBDH)

1.1.1.30 ( BRENDA | IUBMB )

3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.

3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.
Biochem/physiol Actions
3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.
Quality
Contaminants: <0.1% LDH, <5% MDH
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Stabilizers: NADH, Ca2+

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
packaging	vial of 2 mL (10127833001), vial of 5 mL (10127841001)
Торговая марка	Roche
concentration	5 mg/mL
impurities	<0.1% LDH, <5% MDH
optimum pH	6.2-6.9(for reduction), 7-9(for oxidation)
shipped in	wet ice
storage temp.	2-8°C

Узнать цену

Подробнее

5-Bromo-2-deoxy-uridine Labeling and Detection Kit I 11296736001

The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation.
Normally, binding of the antibody is only achieved by denaturation of the DNA. This is usually obtained by exposing the cells to acid, base, or heat. These procedures result in destruction of cell integrity, including cell morphology and surface and cytoplasmatic markers.
The BrdU Labeling and Detection Kit I avoids these problems. The antibody preparation contains specific nucleases which allows access to BrdU after fixation in acidic ethanol. Therefore also simultaneous detection of other markers (double staining) is possible.
Specificity
Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.

Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU. The antibody to BrdU supplied with the kit contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments, which allow binding of the antibody to BrdU without destruction of the cellular morphology. A fluorescein-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. Subsequently, the sample is evaluated using an immunofluorescence microscope.

Safe: No radioisotopes are used
Easy to perform: Follows a standard immunofluorescence protocol
Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU
Flexible: Allows double-labeling protocols

BrdU Labeling and Detection Kit has been used for the detection of 5-bromo-2-deoxy-uridine (BrdU) incorporated into cellular DNA.
Packaging
1 kit containing 5 components.
Preparation Note
Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [3H]-thymidine, into cellular DNA, followed by autoradiography. Alternatively, 5-bromo-2-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Working solution: BrdU labeling medium
Dilute BrdU labeling reagent 1:1000 with sterile cell culture medium (final concentration 10µM).
Note: For in vivo labeling undiluted BrdU labeling reagent (1 to 2ml/100 g body weight) is needed.
Prepare shortly before use.
Anti-BrdU working solution
Dilute anti-BrdU solution 1:10 with Incubation buffer.
Prepare shortly before use.
Anti-mouse-Ig-fluorescein stock solution
Dissolve anti-mouse-Ig-fluorescein solution in 1ml double-dist. water.
Anti-mouse-Ig-fluorescein working solution
Dilute anti-mouse Ig-fluorescein stock solution 1:10 with PBS. If an extended storage is desired, add BSA (bovine serum albumin), 10 mg/ml.
Prepare shortly before use.
Washing buffer
Dilute Washing buffer concentrate (10x) (bottle 2) 1:10 with double-dist. water.
Storage conditions (working solution): BrdU labeling medium
Store undiluted (1000x) medium in aliquots at -15 to -25°C.
Anti-BrdU working solution
Store undiluted antibody at -15 to -25°C.
Anti-mouse-Ig-fluorescein stock solution
Stable at 2 to 8°C
Washing buffer
Stable at 2 to 8°C
Sample material: Cell culture: adherent cells, suspension cells, organ, or explant cultures. Tissue sections (after in vivo labeling with BrdU).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for ≤100 tests
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H315 - H317 - H319 - H412
pcodes	P261 - P264 - P273 - P280 - P333 + P313 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

5-Bromo-2-deoxy-uridine Labeling and Detection Kit II 11299964001

Immunohistocytochemical assay for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.
Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Specificity
Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.

The kit is used for the detection of BrdU incorporated into cellular DNA by immunohistocytochemistry.
5-Bromo-2-deoxy-uridine Labeling and Detection Kit II has been used in:

labeling of tooth roots for histology
immunostaining of mice frontal sections
immunofluorescence imaging of hepatocellular carcinoma sections

Features and Benefits

Safe: No radioisotopes are used.
Easy to perform: Follows a standard immunohistochemistry protocol.
Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU.
Flexible: Allows double-labeling protocols.

Packaging
1 kit containing 7 components.
Principle
Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU, which contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments that allow binding of the antibody to BrdU. Next, an alkaline phosphatase (AP)-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. The sample is then incubated with the AP substrate and NBT/ BCIP, which is metabolized to form a colored reaction product. The sample is evaluated using a phase-contrast microscope.
Preparation Note
Working concentration: Working concentration of the labeling reagent corresponds to the WC of the In Situ Cell Proliferation Kits.
Sample material:
Cell culture: adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for ≤100 tests
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS02,GHS07,GHS08
signalword	Danger
hcodes	H226 - H312 + H332 - H317 - H319 - H360D
pcodes	P201 - P210 - P261 - P280 - P308 + P313 - P370 + P378
WGK Germany	WGK 2
Flash Point F	136.4 °F
Flash Point C	58 °C

Узнать цену

Подробнее

5-Bromo-2-deoxy-uridine Labeling and Detection Kit III 11444611001

Proliferation in cell populations may be studied by incorporating the radioisotope [3H]-thymidine into cellular DNA. The amount of radioactive thymidine incorporated is determined by scintillation counting. Alternatively , 5-bromo-2-deoxy-uridine may be incorporated into cellular DNA. The amount of BrdU incorporated is determined by a standard ELISA protocol, which involves "tagging" the incorporated nucleotide with an anti-BrdU antibody. 96-well microplate cell ELISA for the detection of 5-bromo-2-deoxy-uridine (BrdU) incorporated into cellular DNA. Nonradioactive alternative for [3H]-thymidine based DNA synthesis and cell proliferation assays.
Contents

BrdU Labeling Reagent, 1,000x concentrated
Washing Buffer concentrate, 10x concentrated
Incubation Buffer
Nucleases
Anti-BrdU-POD, Fab fragments
Substrate Buffer
ABTS Substrate
Substrate Enhancer

Specificity
Anti-BrdU-peroxidase, Fab fragments, specifically bind to 5-bromo-2′-deoxy-uridine incorporated into DNA. It shows no cross-reactivity with any endogenous cellular components, such as thymidine or uridine.

The kit is used for the quantitative determination of BrdU incorporated into cellular DNA using a 96-well microplate cell ELISA format.
5-Bromo-2-deoxy-uridine Labeling and Detection Kit III has been used in cell proliferation assay, BrdU assay and proliferation assay.
Features and Benefits

Safer, since the kit does not use radioisotopes.
Accurate, since results generated with this assay strongly correlate to those obtained with the [3 H]-thymidine method. (See Figure 2 below.)
Sensitive. This assay and the [3 H]-thymidine assay are equally sensitive. (See Figure 2 below.)
Fast, since results can be read with a multi-well ELISA reader, allowing simultaneous processsing of a large number of samples
Easy, since the assay uses a standard cell ELISA protocol.
Economical, since the assay requires no expensive equipment or additional reagents (e.g ., scintillation fluid).

Packaging
1 kit containing 8 components.
Principle
Cells cultured in a 96-well microplate are incubated with BrdU (see Performance). The labeled cells are fixed with ethanol. Prior to incubation with a monoclonal antibody to BrdU, DNA is partially digested with nucleases to allow the antibody to access BrdU. Next, the anti-BrdU antibody [labeled with peroxidase (POD)] is added. Finally, the POD substrate ABTS is added. POD catalyzes the cleavage of ABTS, producing a colored reaction product. The absorbance of the samples (at approximately 405 nm) is determined with a standard microplate (ELISA) reader.
Preparation Note
Working solution: BrdU labeling solution

Dilute BrdU labeling reagent 1 : 90 with sterile PBS or culture medium (resulting concentration: 111 µM BrdU) [e.g., for one 96-well microplate containing 100 µl medium per well, dilute 12 µl BrdU labeling reagent with 1.068 ml sterile PBS].
Note: The BrdU labeling solution should be prepared freshly before use.

Washing buffer
Dilute washing buffer concentrate (10x) 1 : 10 with double-dist. water [e.g., for one 96-well microplate dilute 9 ml washing buffer concentrate (10x) with 81 ml double-dist. water].
Note: If precipitates in Washing buffer, 10x conc. are visible, please incubate the bottle for 10 minutes at 37 °C in a water bath before you prepare Solution II.
Washing buffer is used to:

Prepare the anti-BrdU-peroxidase, working solution
Wash cells after incubation with anti-BrdU-peroxidase

Note: For all other washing steps use PBS or culture medium containing 10% serum [e.g., FCS (fetal calf serum)] to obtain reliable results.
Incubation buffer

Ready-to-use
Used to dilute the nucleases

Nucleases, stock solution
Reconstitute the nucleases in 1.3 ml double-dist. water containing 50% glycerol (w/v).
Nucleases, working solution
Dilute nucleases, stock solution, 1 : 100 with incubation buffer (e.g., for one 96-well microplate dilute 100 µl nucleases, stock solution, with 9.9 ml incubation buffer).
Anti-BrdU-peroxidase, Fab fragments, stock solution

Dissolve anti-BrdU-peroxidase, Fab fragments in 1.25 ml double-dist. water (final concentration: 20 U/ml).
Anti-BrdU-peroxidase, Fab fragments, working solution
Prepare anti-BrdU-peroxidase, Fab fragments, working solution shortly before use. Dilute anti-BrdU-peroxidase, Fab fragments, stock solution 1 : 100 with washing buffer supplemented with 10 mg/ml BSA (bovine serum albumin), [e.g., for one 96-well microplate dilute 100 µl anti-BrdU-peroxidase, Fab fragments, stock solution, with 9.9 ml PBS and BSA (final concentration: 200 mU/ml)].
Peroxidase substrate
Dissolve the ABTS powder in substrate buffer and stir at 15 to 25 °C to obtain a clear solution.
Peroxidase substrate containing substrate enhancer
If a low signal is expected, take an appropriate aliquot of substrate solution and add substrate enhancer , 1 mg/ml and dissolve by stirring for 15 minutes at 15 to 25 °C (e.g., for one 96-well microplate dissolve 10 mg substrate enhancer in 10 ml peroxidase substrate)
Note: The substrate solution containing substrate enhancer is stable for only 4 hours and should, therefore, be freshly prepared before use.
Storage conditions (working solution): BrdU labeling reagent
The undiluted BrdU labeling reagent (1000x) is stable at 2 to 8 °C for 6 months. It is stable stored in aliquots at -15 to -25 °C.
Washing buffer
Stable at 2 to 8 °C for 3 months.
Incubation buffer
Stable at 2 to 8 °C until the expiration date printed on the label
Nucleases, stock solution
Stable at -15 to -25 °C for 6 months.
Nucleases, working solution
must be prepared shortly before use.
Anti-BrdU-Peroxidase, Fab fragments, stock solution
Stable at 2 to 8 °C for 6 months. For long term storage it is recommended to store the solution in aliquots at -15 to -25 °C.
Anti-BrdU-Peroxidase, Fab fragments, working solution
must be prepared freshly before use.
Peroxidase substrate
Stable at 2 to 8 °C for 2 months when stored protected from light.
Peroxidase substrate containing substrate enhancer
must be prepared freshly before use

For life science research only. Not for use in diagnostic procedures.

P261 - P273 - P280 - P333 + P313 - P337 + P313 - P362 + P364

usage	sufficient for ≤1,000 tests
Quality Level	100
specific activity	10000 (Nuclease activity (vial 4))
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

5-Bromo-2-deoxyuridine 10280879001

Empirical Formula (Hill Notation):	C₉H₁₁BrN₂O₅
CAS Number:	59-14-3
Molecular Weight:	307.10
Beilstein/REAXYS Number:	30395
MDL number:	MFCD00006529
PubChem Substance ID:	329749004

It is conventionally used as a marker for S-phase synthesis of DNA.

Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.
5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.
Biochem/physiol Actions
Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.
Quality
Purity: 97% (from N), chromatographically homogeneous
Preparation Note
Working concentration: According to literature for cell culture, a final concentration of 10 µmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C

For life science research only. Not for use in diagnostic procedures.

assay	>97%
Quality Level	100,
mol wt	307.1
packaging	pkg of 1 g
Торговая марка	Roche
mp	191-194 °C (dec.) (lit.)
shipped in	wet ice
storage temp.	2-8°C
SMILES string	OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
InChI	1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
InChI key	WOVKYSAHUYNSMH-RRKCRQDMSA-N

pictograms	GHS08
signalword	Warning
hcodes	H351
pcodes	P201 - P202 - P280 - P308 + P313 - P405 - P501
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable

5/3 RACE Kit, 2nd Generation 3353621001

Rapid Amplification of cDNA ends (RACE) is a prevalent technique used to rapidly obtain full-length cDNA from partially known sequences. The 5/3 RACE kit contains Transcriptor Reverse Transcriptase and recombinant Terminal Transferase. Transcriptor Reverse Transcriptase transcribes full-length cDNA for the highly sensitive and rapid amplification of either 5 or 3 cDNA fragments up to 14 kb. Due to its thermostability (up to +65 °C), it can work with GC-rich templates with high secondary structure. High sensitivity can be achieved using Transcriptor Reverse Transcriptase, resulting in highly efficient cDNA synthesis and the generation of long RACE products. Recombinant Terminal transferase is used to add a homopolymeric A-tail to the 3 end of the cDNA. The poly(A)+ tail decreases the likelihood of inappropriate truncation by the oligo(dT)-anchor primer, and overcomes the weaker A/T compared to the G/C hybridization. Moreover, longer stretches of A residues are required before the oligo(dT)-anchor primer can hybridize to an internal site and can truncate the amplification product. Tailed cDNA is amplified by PCR using a gene-specific primer and the oligo(dT)-anchor primer. The obtained cDNA is further amplified by a second PCR using a nested specific primer and the PCR-anchor primer, allowing RACE products to be cloned into an appropriate vector for subsequent studies.
Specificity
Heat inactivation: Terminal Transferase: 70 °C for 10 minutes
Transcriptor Reverse Transcriptase: 85 °C for 5 minutes

5/3 RACE Kit, 2nd Generation is suitable for

Structural and expression studies of RNA molecules
Generating full-length cDNAs
Isolation and characterization of 5 or 3 ends from low-copy RNA messages
first strand cDNA synthesis
Amplification and further cloning of rare mRNAs
Use in conjunction with exon-trapping methods
Products of the RACE reaction can be directly sequenced without cloning

Features and Benefits
The control reaction includes transcription of the control RNA into first-strand cDNA using the control primer neo1/rev. Amplification of the cDNA is performed using the control primer neo2/rev and neo3/for to obtain a 157-bp PCR product. Tailing of the purified cDNA is performed with dATP. Amplification of the tailed cDNA is performed with oligo dT-anchor primer and the control primer neo2/rev to obtain a 293-bp PCR product.

Robust performance: Recombinant Transcriptor Reverse Transcriptase allows procession through regions of difficult secondary RNA structure.
Convenient: Function and expression studies of either 5 or 3 end of the RNA can be performed with the same kit.
Reliable: dA tailing of cDNA with Recombinant Terminal Transferase decreases the likelihood of inappropriate truncation.
Reproducible: Oligo dT-anchor primer with non 3dT ensures correct binding to the inner end of the poly (A) tail.
Produce long fragments: Generate cDNA up to 14kb in length with Transcriptor Reverse Transcriptase.

Packaging
1 kit containing 12 components

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 10 reactions
Quality Level	100,
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

7-Deaza-2-deoxy-guanosine-5-triphosphate 10988537001

Empirical Formula (Hill Notation):	C₁₁H₁₇N₄O₁₃P₃
CAS Number:	101515-08-6
Molecular Weight:	506.19
MDL number:	MFCD00171614
PubChem Substance ID:	329749263

7-deaza-2′-deoxy-guanosine-5′-triphosphate is a nucleotide analog that is a candidate substrate for processive telomerase. The use of this analog improves PCR (polymerase chain reaction) yields. This compound also makes sequencing of GC rich sequences easier, especially if template amount is low and/or DNA quality is not good.
The product contains 7-Deaza-2′-deoxy-guanosine-5′-triphosphate as a 10 mM solution of the lithium salt.

7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).
Preparation Note
Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP.
Analysis Note
Absorbance determination: A250/A260, A280/A260, A290/A260
Absorption: 25 A260 units of product correspond to 1 mg.

When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished.
PCR amplification efficiency is generally not affected by 7-Deaza-dGTP.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	≤4% (7-Deaza-dGDP, HPLC), 95% (7-Deaza-dGTP, HPLC)
form	solution
packaging	pkg of 200 μL (10 mM, 2 μmol)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C
SMILES string	NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
InChI	1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
InChI key	DLLXAZJTLIUPAI-UHFFFAOYSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

ABTS™ 10102946001

Empirical Formula (Hill Notation):	C₁₈H₂₄N₆O₆S₄
CAS Number:	30931-67-0
Molecular Weight:	548.68
Beilstein/REAXYS Number:	7329461
MDL number:	MFCD00010404
PubChem Substance ID:	329748893

2,2-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.

ABTS (C18H16N4O6S4-(NH4)2) is a peroxidase substrate for ELISA.
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Quality
Purity: >98% (absorbance), chromatographically homogeneous
Preparation Note
Working concentration: ABTS should be dissolved as follows:

100 mg ABTS in in 100ml 3.25 mM sodium perborate, 39.8 mM citric acid, 60 mM disodium hydrogen phosphate, pH 4.4-4.5 (see also PI of SA-peroxidase). This buffer corresponds to the single reagent ABTS-Buffer, Cat. No. 1204530. The formed product is green and soluble in water. Measurement at 405 nm.
Dilution of ABTS:
ABTS/ buffer solution (9.1 mM ABTS; pH 5.0): 99,9 mg ABTS in 20 ml potassium phosphate buffer (0.1M; pH 5.0).
Storage and Stability
Store at 2 to 8 °C. (It is also possible to store at -15 to -25 °C)

For life science research only. Not for use in diagnostic procedures.

assay	>98%
Quality Level	100,
form	crystalline
mol wt	548.68
packaging	pkg of 2 g
Торговая марка	Roche
solubility	50 mg/mL
_max	414 nm
shipped in	wet ice
storage temp.	2-8°C
SMILES string	[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
InChI	1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI key	OHDRQQURAXLVGJ-AXMZSLBLSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

ABTS™ Buffer 11204530001

2,2-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.

ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
Preparation Note
Working concentration: Flow cytometry: 0.5 µg/100 µl (106 cells); Immunohistocytochemistry: 50 µg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 125 mL
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

ABTS™ Buffer 11112597001

2,2-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA, using ABTS, is a highly sensitive, specific and reproducible technique.

ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
Preparation Note
Working solution: Dissolve the contents of one bottle substrate for ABTS in 100 ml double-distilled water. This concentrate is stable when stored in aliquots at -15 to -25 °C.
To obtain the working solution, dilute one aliquot in a ratio of 1:10 with double-dist. water. Dissolve one ABTS tablet (50 mg) in 50 ml of the working solution. This solution has a light-green color. In microtiter plates the solution is colorless. A405 nm/1 cm should be < 0.16.
Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

For life science research only. Not for use in diagnostic procedures.

form	powder
Quality Level	100,
packaging	pkg of 16.7 g
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

ABTS™ Solution 11684302001

ABTS™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.

ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.
Biochem/physiol Actions
2,2-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS™ is a highly sensitive, specific and reproducible technique This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.
Physical form
Reaction product:
Color: green
Evaluation: photometric (405nm)
Analysis Note
Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).

For life science research only. Not for use in diagnostic procedures.

form	solution (ready-to-use)
Quality Level	100,
usage	sufficient for 1,500-3,000 assays (ELISA)
packaging	pkg of 3 x 100 mL
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

ABTS Tablets are used as substrates in enzyme immunoassays with horseradish peroxidase as a marker enzyme. It is widely used in spectrophotometric methods to measure antioxidant activities of food and beverages.

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

ABTS™ Tablets

ABTS Tablets are the ideal substrate for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme.
Features and Benefits

ABTS is soluble in aqueous and organic solvents
ABTS method is suitable for various pH levels
Samples react with ABTS quickly and reach stability within 30 minutes

Packaging

11204521001 (5 mg/tablet; each tablet is for 5 mL substrate solution)
11112422001(50 mg/tablet; each tablet is for 50 mL substrate solution)

Preparation Note
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of the ABTS buffer solution. This solution has a light-green color. In microtiter plates, it is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
Analysis Note
Evaluation: photometric (405nm)

For life science research only. Not for use in diagnostic procedures.

form	tablet
Quality Level	100,
packaging	pkg of 20 tablets (11112422001[50mg]), pkg of 20 tablets (11204521001[5mg])
Торговая марка	Roche
color	green
solubility	water: soluble
_max	414 nm
shipped in	wet ice
storage temp.	2-8°C

Acetyl-Coenzyme A, trilithium salt

Acetyl-Coenzyme A

Acetyl-Coenzyme A is used to assay CAT enzyme activity in cell extracts using radioisotopes. CAT enzyme activity in cell extracts catalyzes the transfer of acetyl groups from acetylcoenzyme A to chloramphenicol. A number of assays have been developed to measure CAT activity in cell extracts. Acetyl-Coenzyme A has also been used to determine citrate synthase activity.
Biochem/physiol Actions
Acetyl-Coenzyme A (Ac-CoA) is the end product of glycolysis and takes part in the Ac-CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis. It also takes part in fatty acid biosynthesis and catabolism of polyamines like spermine and spermidine. A low level of Ac-CoA leads to a loss in glial cell and neuronal function. Ketone bodies and triglycerides give rise to Ac-CoA on hydrolysis and this indirectly leads to increased histone acetylation.
Preparation Note
Working solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5).
Storage conditions (working solution): -15 to -25 °C
50 mg/ml in phosphate buffer, pH 7, is stable for 3 weeks at -15 to -25 °C. Unfrozen solutions should be immediately used.

For life science research only. Not for use in diagnostic procedures.

description	Chemical Formula: C₂₃H₃₅N₇O₁₇P₃SLi₃
Quality Level	100,
assay	2% (lithium), 85% (Enzymatic and Absorbance)
mol wt	M_r 809.6 (acetyl-CoA), M_r 827.4 (acetyl-CoA-Li₃)
packaging	pkg of 010 mg (10101893001), pkg of 200 mg (11585371001), pkg of 050 mg (10101907001)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

Acid Phosphatase 10108227001

3.1.3.2 ( BRENDA | IUBMB )

Orthophosphoric-monoester phosphohydrolase (acid optimum).
Acid phosphatase is a non-specific phosphomonoesterase, found in the cytoplasmic and cell wall sections of swelling potato tubers. It possesses several acidic amino acid residues. It is found to be present in up to six isoforms with varying degree of glycosylation. The major potato isoform is a homodimer having a molar mass of 96kDa.
Biochem/physiol Actions
Acid phosphatase maintains the concentration of inorganic phosphate. It hydrolyzes orthophosphate monoesters under acidic conditions and also takes part in tuber development in potatoes. It eliminates phosphate groups from phosphoproteins, such as casein, riboflavin binding protein, pepsinogen, ovalbumin, and phosvitin.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	~2 units/mg protein (at 25 °C with 4-nitrophenyl phosphate as the substrate)
packaging	pkg of 500 mg
Торговая марка	Roche
optimum pH	4.9-5.6
shipped in	wet ice

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Actin RNA Probe, DIG-labeled 11498045910

This antisense RNA probe (human -actin) is in vitro transcribed in the presence of digoxigenin (DIG)-UTP. The transcript has a length of 588 bases. 550 bases are complementary to the 5 region of human -actin mRNA (nucleotides 69 to 6l8, EMBL: HSAC07). The additional 25 bases at the 5 end, and the 13 additional bases at the 3 end of the transcript are specific for the promoter/polylinker region of the transcription vector.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Actin RNA Probe, DIG-labeled, is specifically useful for evaluating the quality and quantity of various RNA species and can be used:

in In situ hybridization (for example, as a control in mRNA detection)
for quality control in the construction of cDNA libraries
in northern blot analysis to evaluate RNA from various human cell lines and tissue samples

Components
Actin RNA Probe, DIG-labeled, is supplied in autoclaved, DEPC-treated water.
Sequence
Agarose gel electrophoresis under denaturing conditions and subsequent northern blot analysis reveal a defined band of 588 bases.
Preparation Note
Working solution: Dilute Sample

Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes.
The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification.
Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6.
Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibody
concentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube.

Storage conditions (working solution): During assay, the preparation should be maintained at 0 °C.

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 2 µg
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	No data available
Flash Point C	No data available

The special quality of ATP contains highly purified Adenosine-5′-triphosphate in crystal form.

Adenosine 5-triphosphate disodium salt trihydrate

Empirical Formula (Hill Notation):	C₁₀H₂₀N₅Na₂O₁₆P₃
CAS Number:	51963-61-2
Molecular Weight:	605.19
MDL number:	MFCD00150754
PubChem Substance ID:	329773088

ATP, disodium salt, is used for the luminometric determination of Luc activity in cell extracts.
Preparation Note
Storage conditions (working solution): The reconstituted aqueous solution is stable at 2 to 8 °C for one week.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	99%
mol wt	507.2 (ATP), M_r 551.14 (ATP-Na2)
packaging	pkg of 1 g (10519979001), pkg of 5 g (10519987001)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C
SMILES string	O.O.O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
InChI	1S/C10H16N5O13P3.2Na.3H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;31H2/q;2+1;;;/p-2/t4-,6-,7-,10-;;;;;/m1...../s1
InChI key	MWEQTWJABOLLOS-AZGWGOJFSA-L

Adenosine Deaminase (ADA) 10102105001

3.5.4.4 ( BRENDA | IUBMB )

Adenosine deaminase (ADA) is a zinc metalloenzyme.
Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.

Adenosine deaminase has been used to pre-treat primary Kupffer cells.
Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.
Biochem/physiol Actions
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.
Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.
Quality
Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each
Physical form
Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6
Preparation Note
Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
specific activity	~200 units/mg protein (At 25 °C with adenosine as the substrate.)
mol wt	230-440 kDa (high), 30-47 kDa (low )
packaging	pkg of 2 mL (10 mg)
Торговая марка	Roche
optimum pH	7.0-7.4
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Adenosine Deaminase (ADA) 10102121001

3.5.4.4 ( BRENDA | IUBMB )

Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.
Chemiluminescent adenosine assay.
Biochem/physiol Actions
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.
Quality
Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~200 units/mg protein (At 25 °C with adenosine as the substrate.)
packaging	pkg of 1 mL (10 mg)
Торговая марка	Roche
optimum pH	7.0-7.4
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

Agarose Gel DNA Extraction Kit 11696505001

For the elution of DNA fragments from agarose gels.
The Agarose Gel DNA Extraction Kit provides convenient isolation of high-quality DNA in large amounts.
By combining agarose gel electrophoresis and extraction, you can easily concentrate dilute, aqueous DNA solutions. After processing with the kit, the DNA can be recovered in a volume of 20 to 50 µL. Isolated DNA fragments are free of inhibitors that could affect common downstream procedures. For example, recovered DNA can be efficiently ligated into cloning vectors or labeled to high specific activity. Restriction digests proceed without inhibition.

Agarose Gel DNA Extraction Kit has been used to extract full-length (CHRNA_v2) and shortest (CHRNA_v3) amplicons from agarose gels.
The Agarose DNA Extraction Kit efficiently isolates both small and large DNA fragments from standard or low melting point agarose. Recovered DNA fragments are suitable for:

Ligation and transformation
Enzymatic restriction
Random primed or nick translation labeling methods
Sequencing
PCR/long PCR
Cloning
Concentrate dilute nucleic acid solutions

Features and Benefits

Quick and simple.

Extract high yields of DNA in only 45 minutes, with few hands-on steps.

Combine with different agaroses and buffer systems.

Low melting point agarose is not required.

Purify efficiently.

Highly specific binding of DNA allows easy removal of impurities.

Isolate large DNA fragments without shearing.

Silica particles are uniform in size and have smooth surfaces, ensuring recovery of intact DNA fragments up to 100 kb.

Isolate oligonucleotides >=20 bp.

Narrow size distribution of the silica particles and absence of fines ensure high binding capacity of the matrix.

Avoid enzymatic inhibition.

No fine particles are observed in the suspension. The recovered product will not inhibit subsequent enzymatic reactions.
Components

Silica Matrix, for 100 standard reactions
Solubilization Buffer
Nucleic Acid Binding Buffer
Wash Buffer

Quality
DNA Molecular Weight Marker II is separated in a 0.8% agarose gel and the 546-, 2,322-, and 6,557 bp fragments are isolated with the kit components. The expected amount of DNA is recovered for each fragment. Depending on parameters such as fragment length, preparation, and purity of the applied DNA, up to 80% recovery is achieved. DNA fragments are digested with restriction enzymes. No inhibition of DNA digestion is observed.
Preparation Note
A solubilization buffer containing a chaotropic salt (sodium perchlorate) dissolves an agarose gel slice that contains a DNA fragment. In the presence of the chaotropic salt, the DNA fragment binds selectively to silica matrix. The DNA remains bound while a series of rapid wash-and-spin steps remove contaminating small molecules. Finally, low-salt elution removes the DNA from the silica matrix. The process does not require DNA precipitation, organic solvent extractions, or extensive handling of the DNA.

High Pure Technology and Silica Adsorption Kits,
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for up to 100 reactions

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

pictograms	GHS03,GHS07
signalword	Danger
hcodes	H271 - H302
pcodes	P210 - P220 - P264 - P280 - P370 + P378 - P371 + P380 + P375
RIDADR	UN 1502 5.1 / PGII
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Agarose LE

Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.
Quality
Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Agarose LE is especially suited for analyzing fragments between 0.2 and 15 kb in: Analysis of PCR products
Examination of restriction endonucleases
Digests of plasmid, cosmid, and phage DNA
Electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde

For life science research only. Not for use in diagnostic procedures.

form	powder
packaging	pkg of 100 g (11685660001), pkg of 500 g (11685678001)
Торговая марка	Roche
shipped in	ambient
storage temp.	20-25°C

Agarose MP, a multi purpose agarose, is developed for analytical and preparative electrophoresis of nucleic acids and separation of high molecular weight DNA via pulse field gel electrophoresis (PFGE).It is also used for preparative electrophoresis and quantitative recovery of biologically active DNA. Agarose MP has also been used along with other supplements to store embryos in different developmental stages.
Quality
Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Agarose MP

Agarose MP can produce high strength gels (e.g., the gel strength of a 1% agarose MP gel is ?1800 g/cm2). Even gels that have low concentrations of agarose MP are strong enough to be used for electrophoresis. The agarose matrix of such low concentration gels allows high molecular weight molecules to migrate rapidly without significant restriction.
Agarose MP is particularly well suited for pulsed-field gel electrophoresis, because it allows short separation times for large DNA molecules, while maintaining the high resolution required for effective analysis.
For life science research only. Not for use in diagnostic procedures.

form	powder
packaging	pkg of 100 g (11388983001), pkg of 500 g (11388991001)
Торговая марка	Roche
shipped in	ambient
storage temp.	20-25°C

Aldehyde Dehydrogenase 10171832001

1.2.1.5 ( BRENDA | IUBMB )

Aldehyde dehydrogenase (ALDH) is a soluble enzyme and its activity depends on potassium ions and cysteine. The family of ALDH comprises of 19 genes in human. ALDH gene is expressed in the nucleus, cytosol, mitochondria and endoplasmic reticulum of the cell. ALDH is a component of nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH) recycling systems.

Aldehyde Dehydrogenase has been used to evaluate fiber-optic biosensors for measurement of acetaldehyde (AcH) in liquid phase (AcH biosensor).
Biochem/physiol Actions
Aldehyde dehydrogenase (ALDH) regulates the non-P450 aldehyde reduction enzyme system. It protects the cell from the effects of toxic aldehydes. Mutations in this gene are associated with Sjogren Larsson syndrome, Larsson syndrome, type II hyperprolinemia and cancer. ALDH takes part in the oxidation of aldehydes to acids. ALDH-2 lowers cardiac ischemia, which arises due to myocardial infarction or post cardiac surgery.
Quality
Contaminants: <0.01% “NADH oxidase”, ADH, and LDH each
Physical form
Enzyme is stabilized with potassium phosphate buffer, pH approximately 6.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	yeast
form	lyophilized
specific activity	~20 units/mg protein (At 25 °C with acetaldehyde as the substrate.)
packaging	pkg of 250 U
Торговая марка	Roche
optimum pH	8.75
relevant disease(s)	cancer
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H332
pcodes	P261 - P271 - P304 + P340 + P312
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

Alkaline Phosphatase 10567752001

3.1.3.1 ( BRENDA | IUBMB )

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Amino groups: 8-16 moles/mole enzyme

Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.
Quality
Purity: single peak (HPLC)
Sequence
Purification procedure
AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.
In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP
No information in literature about -SH groups in AP.
Carbohydrate structure
Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.
NH2- and carbohydrate estimation
Results of NH2- and carbohydrate estimation are available.
Unit Definition
Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5]
1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]
Physical form
Solution, ready-to-use, 10mg/ml
Preparation Note
Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C.
1:10 dilution in water will give stability for 1 week at 4°C.
Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.
Storage and Stability
Store at 2 to 8 °C. (Do not freeze)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
specific activity	~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)
packaging	pkg of 1.5 mL (15 mg)
Торговая марка	Roche
optimum pH	9.8
pH range	9.0 - 11.0
shipped in	wet ice

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Alkaline Phosphatase (AP)

3.1.3.1 ( BRENDA | IUBMB )

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Alkaline phosphatase catalyzes the removal of phosphate group from various compounds that are phosphorylated. It hydrolyzes 5-monophosphate groups from both DNA and RNA. It can also hydrolyze 5-diphosphate and 5-triphosphate groups from RNA.
Specificity
Heat inactivation: AP activity is destroyed irreversibly by incubating the enzyme at 65 °C.
Denaturation is dependent on protein concentration - using less than 10 U/ml (0.1 mol/l glycine buffer, pH 10.5, 1 mmol/l MgCl2 and 0.1 mmol/l ZnCl2), AP is inactivated by a 10 minutes incubation at 65 °C.

Selective cleavage of terminal phosphate groups from oligonucleotides and in protein nucleotide exchange and deliverance. It has also been used to phosphorylate samples to distinguish between phosphate moieties present as phosphorylated glycans and phosphorylated amino acid residues.
Quality
Contaminants: <0.0004% PDE, <0.004% ADA and A-5-MP deaminase, each
Sequence
AP is a dimer of identical or nearly identical subunits; each of which contains 2 molecules of Zn2+ , one tightly bound and necessary for structural stability, the other loosely bound and required for caytalytic activity. The active site contains a reactive serine.
In some mammals (e.g., humans), there are at least 3 distinguishable isoenzymes: the intestinal, the placental and the form found in bone/liver/kidney.
Unit Definition
Unit Conversion: 1 Roche unit (tested at +25 °C) corresponds to ~2 to 2.5 Sigma units (tested at +37 °C).
AP activity is not expressed in Armstrong units; a conversion factor for international units is not known.
Approx. 3.8 U (grade I AP) or 4.0 U (grade II AP) [+37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5].
Biochemica Information. 87 (grade I AP) :
1.0 U [+37 °C, QC assay conditions] = 0.79 U [+30 °C, QC assay conditions] = 0.57 U [+25 °C, QC assay conditions]
Physical form
Suspension in 3.2 M ammonium sulfate solution, 1 mM MgCl2, 0.1 mM ZnCl2, pH approximately 7
Preparation Note

Activator: Divalent metal ions (Mg2+, Co2+, Mn2+)
Amino alcohols (2-amino-2-methyl-1-propanol, diethanolamine)

Note that high concentrations of amino alcohols can as well act as competitive inhibitors (e.g., monoethanolamine in diethanolamine).
Storage conditions (working solution): The suspension is offered at a c= approx. 5 mg/ml.
If this suspension is diluted only 5 to 10-fold with further 3.2 M ammonium sulfate solution, 1 mM MgCl2, 0.1 mM ZnCl2, pH approx. 7, we would expect from general experience that a stability for some weeks might be given provided it is stored at 4 °C (no guarantee!).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~2000 units/mg protein (At 37 °C with 4-nitrophenyl phosphate as the substrate, and diethanolamine as the buffer (400 U/mg at 25 °C with 4-nitrophenyl phosphate as the substrate, and glycine as the buffer))
packaging	pkg of 1,500 U (10108138001), pkg of 7,500 U (10108146001)
Торговая марка	Roche
optimum pH	8.0-10.5
absorption	7.6 at 278 nm (10 mg AP/ml)
shipped in	wet ice
storage temp.	2-8°C

Alkaline Phosphatase recombinant, highly active 3359123001

3.1.3.1 ( BRENDA | IUBMB )

The recombinant, highly active Alkaline Phosphatase possesses the same amino acid sequence as the native, bovine-sourced Alkaline Phosphatase. No animal-derived components are used in the production process. This eliminates the risk of Bovine Spongiform Encephalopathy (BSE) and other infections caused by animals.
The recombinant enzyme guarantees superior lot-to-lot consistency and completely eliminates the risk of BSE.
Alkaline phosphatase (ALP) is a hydrolase enzyme. It is a highly active form in calf intestinal mucosa. The calf intestinal alkaline phosphatase is a dimeric glycoprotein with a molecular weight of about 140kDa.
Specificity
Alkaline phosphatase catalyzed the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccarides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as enzyme purity, enzyme concentration in the assay, buffer, pH etc.

Alkaline Phosphatase can be coupled to other proteins via its amino and carbohydrate groups. The enzyme solution can be used directly for conjugation without prior dialysis. For conjugation of AP with IgG, a ration of 2.5:1 is recommended. The application of the conjugated enzyme varies from ELISAs and microarrays to western blot analysis.
Biochem/physiol Actions
Alkaline phosphatase (ALP) is responsible for removing phosphate groups at the 5- and 3- positions from many types of molecules, such as nucleotides, proteins, and alkaloids. It can also modify lipopolysaccharides by dephosphorylation of the lipid A moiety, thereby making it less toxic than the unmodified lipid A.
Specifications
≥7,000 U/mg (+37°C; pNPP; DEA)
Physical form
Solution in 3 M NaCl, pH 7.6, 5 mM magnesium chloride, 0.1 mM zinc chloride, 30 mM triethanolamine.
Preparation Note
Activator: Mg2-, Co2- and Mn2-ions

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	95% (HPLC)
form	buffered aqueous solution (ready-to-use, 20 mg/ml)
specific activity	7000 units/mg protein (At 37 °C with pNPP and DEA as the substrates)
packaging	pkg of 10 mg (500 µl)
Торговая марка	Roche
concentration	20 mg/mL (ready-to-use)
optimum pH	8.0(Stability), 9.8(Activity)
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

AMP-PNP 10102547001

Empirical Formula (Hill Notation):	C₁₀H₁₇N₆O₁₂P₃ · xLi⁺ · yH₂O
CAS Number:	25612-73-1
Molecular Weight:	506.20 (free acid basis)
Beilstein/REAXYS Number:	6047109

AMP-PNP(adenylyl-imidodiphosphate) is an artificial substrate of enzyme, adenylate cyclase. Adenylate cyclase helps to hydrolyze adenylyl-imidodiphosphate slowly than ATP.
AMP-PNP (adenylyl-imidodiphosphate) is a non-hydrolysable analogue of ATP. It inhibits fast axonal transport and facilitates the interaction between membranous organelles and microtubules.

AMP-PNP is a competitive inhibitor of most ATP-dependent systems. A good substrate for enzymes hydrolyzing between the - and -phosphorus atom, yet is resistant to enzymes cleaving between the - and -phosphorus atom. Substrate of snake venom phosphodiesterase and adenylate cyclase.
AMP-PNP has been used for two-motor fluorescence assay.
AMP-PNP has been used in the preparation of nucleotide solutions, two-motor fluorescence assay, preparation of flow-cells with kinesin and cascade binding assays.
Features and Benefits
Contents
88% AMP-PNP (from N), 4.5% lithium, 6% water
Quality
Contaminants: 1.5% Pi , <0.1% ATP (enzymatically)
Storage and Stability
Store at -15–-25 °C. (Store dry!)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	powder
mol wt	M_r 506.2 (ANP-PNP), M_r 529.9 (AMP-PNP-Li₄)
packaging	pkg of 25 mg
Торговая марка	Roche
shipped in	dry ice
SMILES string	[Li+].[Li+].[Li+].[Li+].[H]O[H].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)NP([O-])([O-])=O)[C@@H](O)[C@H]3O

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Amyloglucosidase

3.2.1.3 ( BRENDA | IUBMB )

Amyloglucosidase, a 1,4--D-glucan glucohydrolase, is a disaccharidase–type -glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.
Specificity
Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Amyloglucosidase from Aspergillus niger is used for the determination of starch.
Biochem/physiol Actions
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the -D-(1-4) and -(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

Western blot: 1 to 10 µg/ml

Working solution: Tris-buffered saline containing 0.1% Tween 20.
Storage conditions (working solution): 2 to 8 °C

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate), ~6 units/mg protein (At 25 °C with glycogen as the substrate.)
mol wt	97 kDa
packaging	pkg of 3,500 U (11202367001), pkg of 500 U (11202332001)
Торговая марка	Roche
optimum pH	4.6-4.8
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS08
signalword	Danger
hcodes	H334
pcodes	P261 - P284 - P304 + P340 - P342 + P311 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Amyloglucosidase 10102857001

3.2.1.3 ( BRENDA | IUBMB )

Amyloglucosidase is synthesized by several Aspergillus genus species. It is a disaccharidase–type -glucosidase. This enzyme is an exo-enzyme and one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.
Specificity
Cleaves terminal glucoses that are 1,4- or 1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.
Heat inactivation: Heat inactivation is recommended at 80 °C for 45 minutes, followed by rapidly cooling down.

Amyloglucosidase from Aspergillus niger can be used for the hydrolyzation of terminal 1,4- and 1,6-glucosidic bonds (glucose-glucose bonds) in polysaccharides (e.g., starch, dextrins, glycogen), removing glucose units sequentially from the non-reducing end of the molecule. The enzyme will also cleave maltose and maltosides (maltotriose, maltotetraose, etc.).
Biochem/physiol Actions
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the -D-(1-4), the -D-(1-6), and the -D-(1-3) glucosidic bonds of oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry for the commercial production of D-glucose from corn syrups.
Unit Definition
Unit Conversion: One unit (+25 °C; glycogen as substrate) corresponds to 8.6 U (+60 °C; starch as substrate).
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~14 units/mg protein (At 25 °C with glycogen as the substrate; standardized with BSA.)
mol wt	M_r 97 kDa
packaging	pkg of 10 mL (10102857001 [100 mg])
Торговая марка	Roche
parameter	55 °C optimum reaction temp.
optimum pH	4.6-4.8
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Annexin-V-FLUOS 11828681001

Annexin-V-FLUOS is a phospholipid-binding protein with a high affinity for phosphatidylserine (PS). Detection of cell-surface PS with annexin-V thus serves as a marker for apoptotic cells. Analysis may be by flow cytometry or by fluorescence microscopy.
Specificity
Annexin-V binds in a Ca2+-dependent manner to negatively charged phospholipid surfaces, and shows high affinity for phosphatidylserine.

In the early stages of apoptosis, changes occur at the cell surface. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner part of the plasma membrane to the outer layer, by which PS becomes exposed at the external surface of the cell.
Annexin-V-FLUOS detects apoptotic cells in suspension cells using flow cytometry (FACS) or fluorescence microscopy, by binding to phosphatidylserine on the outer leaflet of apoptotic cell membranes. A simultaneous labeling with, e.g., propidium iodide (PI) is needed for differentiation of apoptotic from necrotic cells.
Other secondary labeling is possible, e.g., membrane surface staining with a phycoerythrin or TRITC-labeled monoclonal antibody for further cellular characterization.
Preparation Note
Working concentration: Amount of rGFP recommended for:

SDS-PAGE (Coomassie stained): 1µg
Western blot: 5ng

Storage conditions (working solution): Diluted staining solution should be always prepared freshly.
Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system.
Sample material: Cell lines and freshly isolated cells.

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
usage	sufficient for 250 tests
packaging	pkg of 500 μL
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

In the early stages of apoptosis, changes occur at the cell surface. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane to the outer layer, exposing PS at the surface of the cell. Macrophages specifically recognize PS exposed on the cell surface of lymphocytes during the development of apoptosis. The recognition and phagocytosis of apoptotic cells and bodies protects organisms from exposure to cellular compounds that lead to inflammation, which usually accompanies necrosis. Annexin V is a 35.8kDa, Ca2+ -dependent phospholipid-binding protein with a high affinity for PS, making labeled annexin an excellent detection agent.
Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system.
Sample material: Cell lines and freshly isolated cells.
Kit with FLUOS-conjugated annexin-V and propidium iodide for the detection and quantification of apoptosis and differentiation from necrosis at the single-cell level.
Specificity
Annexin-V-FLUOS binds in a Ca2+-dependent manner to negatively charged phospholipid surfaces, and shows high specificity for phosphatidylserine. Therefore, it stains apoptotic and necrotic cells. Propidium iodide stains only the DNA of leaky necrotic cells and allows for a distinction between apoptotic and necrotic cells.

Annexin-V-FLUOS Staining Kit

Labeled annexin-V, with its high affinity for phosphatidylserine (PS), is a sensitive probe for PS exposed on the outer layer of apoptotic cells. Since necrotic cells also expose PS as a result of lost membrane integrity, propidium iodide is utilized as a DNA stain to distinguish necrotic cells from annexin-V-labeled cell clusters. Other secondary labeling is possible, such as membrane-surface staining with phycoerythrin- or TRITC-labeled monoclonal antibodies for further cellular characterization.
Packaging
1 kit containing 3 components
Preparation Note
Working solution: Predilute 20 µl Annexin-V-FLUOS labeling reagent in 1 ml Incubation buffer and add 20 µl Propidium iodide solution.
Note: 1 ml is enough for 10 samples.
Storage conditions (working solution): Diluted staining solution should be always prepared freshly.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 250 tests (11988549001), sufficient for 50 tests (11858777001)
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Anti-Bromodeoxyuridine 11170376001

Anti-Bromodeoxyuridine is a monoclonal antibody to 5-bromo-2′-deoxyuridine-5′-monophosphate.
Specificity
The antibody specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Anti-bromo-deoxyuridine does not crossreact with fluorodeoxy-uridine, nor with any endogenous cellular components such as thymidine or uridine.

The epitope is apparently inside the DNA helix. DNA has to be denatured to ssDNA before antibody efficiently binds to DNA-BrdU.

Anti-bromodeoxyuridine (Anti-BrdU) antibody is suitable for monitoring proliferating cells in blood, tissues, and tumors, as well as for determining BrdU incorporation on a single-cell level using:

Flow cytometry
Immunohistocytochemistry
Cryosections
Paraffin sections

Quality
The antibody is ≥90% pure as determined by SDS-PAGE with Coomassie-blue staining, and by HPLC.
Specifications
Preparation: BALB/c mice were immunized with a bromodeoxyuridine-bovine serum albumin conjugate. Lymphocytes isolated from the spleen were fused with Ag8.653 myeloma cells to create the BMC 9318 clone. The antibody was produced in ascites in BALB/c mice and purified by ion-exchange chromatography.
No. of tests: 250 (Flow cytometry)
Physical form
Solution, stabilized with phosphate buffered saline, pH 7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) gelatin.
Preparation Note
Working concentration: Flow cytometry: 2 µg/ml (0.2 µg/100 µl/106 cells); Immunohistocytochemistry: 6 µg/ml
Working concentration of conjugate depends on application and substrate. Dilutions should be made in PBS (pH 7.4) containing 0.1% BSA to maintain stability of the antibody.
Analysis Note
Anti-Bromodeoxyuridine shows 10% cross reaction with iodo-deoxyuridine, but no cross reaction to fluoro-deoxyuridine.
No cross reaction to any endogenous thymidine or uridine.
Cross reactivity with 5-Br-UTP has not been tested but it is suggested that there is a good chance for reaction, because the only difference is an absent hydroxyl group on the ribose distal to the bromine substitution.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	BMC9318, monoclonal
assay	90% (HPLC and SDS-PAGE)
form	solution
packaging	pkg of 50 µg (500 µl)
Торговая марка	Roche
isotype	IgG1
conjugate	unconjugated
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

Anti-Bromodeoxyuridine-Fluorescein 11202693001

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Anti-bromodeoxyuridine is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells undergoing replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, antibromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously.
Monoclonal antibody to 5-bromo-2′-deoxyuridine-5′-monophosphate conjugated to fluorescein.
Specificity
The antibody specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Antibromo- deoxyuridine does not crossreact with fluorodeoxy- uridine, nor with any endogenous cellular components such as thymidine or uridine.

Apparently, the epitope is inside of the DNA helix. DNA has to be denatured to ssDNA before antibody efficiently binds to DNA-BrdU.

Physical form
Solution, stabilized with phosphate buffered saline, pH 7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) gelatin.
Preparation Note
Working concentration: Flow cytometry: 0.5 µg/100 µl (106 cells); Immunohistocytochemistry: 50 µg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Analysis Note
Anti-Bromodeoxyuridine shows 10% cross reaction with iodo-deoxyuridine, but no cross reaction to fluoro-deoxyuridine.
No cross reaction to any endogenous thymidine or uridine.
Cross reactivity with 5-Br-UTP has not been tested but it is suggested that there is a good chance for reaction, because the only difference is an absent hydroxyl group on the ribose distal to the bromine substitution.

Anti-bromodeoxyuridine antibody is suitable for monitoring proliferating cells in blood, tissues, and tumors, as well as for determining BrdU incorporation on a single-cell level using: Flow cytometry
Immunohistocytochemistry
Paraffi sections
Cryosections

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	BMC9318, monoclonal
assay	90% (HPLC and SDS-PAGE)
form	solution
packaging	pkg of 50 µg (500 µl)
Торговая марка	Roche
isotype	IgG1
conjugate	fluorescein conjugate
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

Anti-Bromodeoxyuridine-Peroxidase, Fab fragments 11585860001

Fab fragments from monoclonal antibody to 5-bromo-2′-deoxyuridine-5′-monophosphate, conjugated with horseradish peroxidase.
Specificity
The antibody reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with cellular DNA that contains incorporated BrdU; it shows no crossreactivity with other cellular components. The antibody may be applied for the qualitative measurement of cell proliferation (BrdU incorporation) on a single-cell level, and for quantitative measurement in a cell population by ELISA.

The epitope is apparently inside the DNA helix. DNA has to be denatured to ssDNA before antibody efficiently binds to DNA-BrdU.

Anti-bromodeoxyuridine antibody is suitable for monitoring proliferating cells in blood, tissues, and tumors, as well as for determining BrdU incorporation on a single-cell level using:

ELISA (600 tests)
Immunohistocytochemistry (150 tests)
Cryosections
Paraffin sections

Specifications
Clone: BMG-6H8
Ig Class: mouse IgG1
No. of tests: 600 (ELISA), 150 (Immunochemistry)
Physical form
Lyophilizate, stabilized
Preparation Note
Working concentration: ELISA: 200 mU/ml (approximately 0.2 U/ml); Immunocytochemistry 500 to 1000 mU/ml (1.5 U/ml).
The concentrations listed in this section should be taken as a guideline.
Working solution: Phosphate buffered saline containing 1% BSA, pH 7.4
Reconstitution
Add 1 ml double-distilled water to a final concentration of 15 U/ml.
Analysis Note
Anti-Bromodeoxyuridine shows 10% cross reaction with iodo-deoxyuridine, but no cross reaction to fluoro-deoxyuridine.
No cross reaction to any endogenous thymidine or uridine.
Cross reactivity with 5-Br-UTP has not been tested but it is suggested that there is a good chance for reaction, because the only difference is an absent hydroxyl group on the ribose distal to the bromine substitution.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	mouse
antibody form	affinity purified immunoglobulin
antibody product type	primary antibodies
clone	BMG6H8, monoclonal
form	lyophilized
packaging	pkg of 15 U
Торговая марка	Roche
isotype	IgG1
conjugate	peroxidase conjugate
shipped in	wet ice
storage temp.	2-8°C

Anti-c-myc is a monoclonal antibody to c-myc peptide (clone 9E10). Anti-c-myc recognizes the 9E10 epitope sequence (EQKLISEEDL), derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not cross-react with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc peptide epitope is introduced into unrelated recombinant proteins by a technique known as epitope tagging.
The cellular myelocytomatosis (c-myc) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The gene encoding it is mapped to human chromosome 8q24. It c-myc is a member of MYC gene family. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that regulates the G1-S cell cycle transition.
Contents:

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

Anti-c-myc

11 667 149 001: lyophilizate, stabilized
11 667 203 001: frozen solution, (5 mg/ml)

Specificity
Anti-c-myc recognizes the amino acid sequence EQKLISEEDL, which was derived from the human c-myc protein. The antibody recognizes its antigenic determinant even when the c-myc peptide epitope is introduced into unrelated recombinant proteins either N-terminal, C-terminal or internal. The monoclonal antibody against the c-myc epitope is well characterized and does not crossreact with other cellular proteins.

Amino acid sequence EQKLISEEDL was derived from the human c-myc protein.

Use Anti-c-myc for the detection of native human c-myc protein and recombinant epitope-tagged proteins that contain the c-myc epitope using:

Dot blots
Immunocytochemistry
Immunoprecipitation
Western blots
Immunohistochemistry.

Biochem/physiol Actions
The cellular myelocytomatosis (c-myc) oncogene has a crucial role in cellular proliferation, differentiation, apoptosis and acts as transcriptional regulator of gene expression. c-myc expression is essential and sufficient to assist most of the cells to enter DNA synthetic (S) phase of the cell cycle. The encoded protein plays a crucial role in vasculogenesis and angiogenesis during cancer development and progression. c-myc interacts with its binding partner Max and activates the transcription of growth promoting genes such as cyclin D2 and ornithine decarboxylase. It also represses the transcription of multiple genes, especially p21 and p27, by binding to the transcription initiator element (Inr) in a complex with Max and either Sp1 (specificity protein 1) or Miz1 (Myc-interacting zinc finger protein 1). Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in poor outcome and invasive treatment when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP).
Quality
Anti-c-myc antibody is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent lot.
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

Western blot: 1 to 10 µg/ml

Working solution: Tris-buffered saline containing 0.1% Tween 20.
Reconstitution
Add 500 µl PBS to a final concentration of 400 µg/ml.
Analysis Note
The monoclonal antibody against the c-myc epitope is well characterized and does not crossreact with other cellular proteins. No cross reaction with murine c-myc.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	9E10, monoclonal
assay	90% (SDS-PAGE)
form	frozen liquid (11667203001), lyophilized (11667149001)
packaging	pkg of 200 µg (11667149001), pkg of 5 mg (11667203001 [1 ml])
Торговая марка	Roche
isotype	IgG1
epitope sequence	EQKLISEEDL
conjugate	unconjugated
shipped in	dry ice, wet ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	does not flash
Flash Point C	does not flash

Anti-c-myc-Peroxidase 11814150001

Anti-c-myc-Peroxidase is a monoclonal antibody to c-myc peptide (clone 9E10), conjugated to horseradish peroxidase. Anti-c-myc recognizes the 9E10 epitope (EQKLISEEDL), derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not cross-react with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc-peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
Specificity
Anti-c-myc-Peroxidase recognizes the 9E10 epitope (EQKLISEEDL), which was derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not crossreact with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc peptide epitope is introduced into unrelated recombinant proteins either N-terminal, C-terminal or internal.

Amino acid sequence EQKLISEEDL was derived from the human c-myc protein.

Quality
Function test: Western blot using a cell line that expresses a recombinant c-myc-tagged protein.
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

Use Anti-c-myc-Peroxidase for the detection of native human c-myc protein and recombinant epitope-tagged proteins that contain the c-myc epitope using: Dot blots
Western blots

Western blot: 1 µg/ml

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	9E10, monoclonal
assay	90% (HPLC and SDS-PAGE)
form	solution
packaging	pkg of 500 µg (500 µl)
Торговая марка	Roche
isotype	IgG1
epitope sequence	EQKLISEEDL
conjugate	peroxidase conjugate
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

Anti-Digoxigenin-AP, Fab fragments 11093274910

Digoxigenin is a hapten, useful in labeling and detection of nucleic acids. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to alkaline phosphatase. Anti-Digoxigenin-AP, Fab fragments are useful for the detection of digoxigenin-labeled compounds.
Specificity
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Heat inactivation: yes

Use Anti-Digoxigenin-AP, Fab fragments for the detection of digoxigenin-labeled compounds using:

cDNA array
Colony/plaque hybridization
Dot blot
ELISA
Gel shift assay
Immunohistocytochemistry
In situ hybridization
Nonradioactive DNA sequencing blot
Northern blot
RNase protection assay
Southern blot
Western blot
Fluorescent in situ hybridization
Section in situ hybridization and whole mount in situ hybridization
Electrophoretic mobility shift assay

Preparation Note
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline:

Dot blot: 150 mU/ml
ELISA: 150 to 300 mU/ml
Immunohistocytochemistry: 250 to 500 mU/ml
In situ hybridization: 1.5 to 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 to 500 mU/ml

Working solution: Northern blot, Southern blot
100 mM Maleic acid, 150 mM NaCl, pH 7.5.
Western blot
50 mM Tris-HCl, 150 mM NaCl, pH 7.5
other applications
100 mM Tris-HCl, 150 mM NaCl, pH 7.5
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Storage conditions (working solution): Diluted conjugate is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
Analysis Note

Cross reactivity to digitoxin and digitoxigenin: <1 %
No cross reactivity with other human estrogen or androgen steroids, e.g. estradiol or testosterone
Cross reactivity with digoxin: not known
Conjugate does not bind to itself at all
Normally one molecule of the conjugate binds to one molecule digoxigenin, although ther are two possible binding sites for digoxigenin
Nonspecific binding to RNA is not expected

For life science research only. Not for use in diagnostic procedures.

biological source	sheep
Quality Level	100,
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	polyclonal
form	solution
packaging	pkg of 200 μL (150 U)
Торговая марка	Roche
isotype	IgG
conjugate	alkaline phosphatase conjugate
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

Anti-Digoxigenin-POD, Fab fragments 11207733910

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from an anti-digoxigenin antibody, conjugated with horse-radish peroxidase (POD). Anti-Digoxigenin-POD, Fab fragments is useful for the detection of digoxigenin-labeled compounds.
Specificity
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Use Anti-Digoxigenin-POD, Fab fragments for the detection of digoxigenin-labeled compounds using:

Dot blot
ELISA
Immunohistocytochemistry
In situ hybridization
Western blot

ELISA: 50 to 150 mU/ml
Immunohistocytochemistry: 250 to 500 mU/ml
In situ hybridization: 1.5 to 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 to 500 mU/ml

Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Reconstitution
Add 1 ml double-distilled water to a final concentration of 150 U/ml.

For life science research only. Not for use in diagnostic procedures.

biological source	sheep
Quality Level	100,
antibody form	F(ab)₂ fragment of affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	lyophilized (clear, brown solution after reconstitution)
packaging	pkg of 150 U
Торговая марка	Roche
conjugate	peroxidase conjugate
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

Anti-GFP 11814460001

Green Fluorescent Protein (GFP) is a spontaneously fluorescent 27kDa protein originally isolated from the jellyfish Aequorea victoria. The molecular cloning of the GFP gene and its subsequent expression in heterologous systems has established GFP as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. Since, GFP requires no additional substrates or cofactors, GFPs green fluorescence can be easily detected using blue or UV light after expression in either prokaryotic or eukaryotic cells. In addition, several mutant forms of GFP with unique spectral properties (e.g., enhanced fluorescence signal and shifts in excitation and emission spectra) have been reported.
Mixture of two high-affinity monoclonal antibodies selected for their performance in detection of GFP and GFP fusion proteins.
Specificity
Subtype: Both clones are Mouse IgG1

Monoclonal antibody for detection of both wild-type and mutant forms of GFP or GFP fusions using:

Immunoprecipitation
Western blots
Immunostaining

Features and Benefits
Contents
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200µg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).
Quality
Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.
Preparation Note
Working concentration: Working concentration of antibody depends on application and substrate.
The following concentrations should be taken as a guideline:

Western blot: 1:1000 dilution
Immunoprecipitation: 2 to 10 µg

Storage conditions (working solution): -15 to -25 °C
Reconstitution
Add 500 µl double distilled water to a final concentration of 0.4 mg/ml.
Rehydrate on ice for 30 minutes.

For life science research only. Not for use in diagnostic procedures.

This product is sold under license from Columbia University. Rights to use this product are limited to research use only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Columbia Innovation Enterprise, Columbia University, Engineering Terrace - Suite 363, new York, New York 10027.

Quality Level	100,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	13.1, monoclonal, 7.1, monoclonal
assay	>90% (HPLC)
form	lyophilized
packaging	pkg of 200 µg
Торговая марка	Roche
isotype	IgG1
conjugate	unconjugated
shipped in	wet ice
storage temp.	2-8°C

Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome.
Specificity
Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Anti-HA (12CA5)

Amino acids 98-106 from the human influenza virus hemagglutinin protein.

Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:

Dot blots
Immunochemistry
Immunoprecipitation
Western blotting

Note: For experiments in which sensitivity is not critical, use Anti-HA (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity (3F10).
Biochem/physiol Actions
Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.
Quality
Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

Dot blot: 0.1 to 1 µg/ml
Immunfluorescence: 1-10 µg/ml
Western blot: 0.1 to 1 µg/ml

Reconstitution
11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	12CA5, monoclonal
assay	>90% (HPLC and SDS-PAGE)
form	frozen liquid (11666606001), lyophilized (11583816001)
packaging	pkg of 200 µg (11583816001), pkg of 5 mg (11666606001 [1 ml])
Торговая марка	Roche
concentration	5 mg/ml (11666606001)
isotype	IgG2b
epitope sequence	YPYDVPDYA
conjugate	unconjugated
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
Flash Point F	does not flash
Flash Point C	does not flash