Pyruvate Kinase (PK) 10109045001

Enzyme Commission (EC) Number:

2.7.1.40 ( BRENDA | IUBMB )

Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate.
Specificity
Specific activity: Approximately 200 U/mg at +25°C with PEP as the substrate.
Pyruvate Kinase (PK) has been used in the kinase assay.
Pyruvate Kinase (PK) has been used as a sample in ATPase Assays.
Biochem/physiol Actions
Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. The enzyme also exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.
Quality
Contaminants: <0.001% GK, <0.002% HK, “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each.
Physical form
Solution in 50% glycerol (v/v), pH approximately 6
Preparation Note
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
specific activity	~200 units/mg protein (At 25 °C with PEP as the substrate.)
packaging	pkg of 1 mL (10 mg)
Торговая марка	Roche
optimum pH	7.0-7.5
shipped in	dry ice
storage temp.	−20°C (−15°C to −25°C)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available