- Производитель:
- Roche
Enzyme Commission (EC) Number: | 2.7.7.7 ( BRENDA | IUBMB ) |
Pwo DNA Polymerase exhibits increased thermal stability with a half life of greater than 2 hours at +100 °C compared to Taq DNA Polymerase with a half life of less than 5 min at this temperature.
Pwo SuperYield DNA Polymerase, originally isolated from Pyrococcus woesei, is a processive 5 3 DNA polymerase with 3 5 exonuclease proofreading activity; 5 3 exonuclease activity is not detectable.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5 mM.
Specificity
Star activity: In cases where star activity is observed and/or the activity of the enzyme in the PCR mix is low, purify the amplification product prior to the restriction enzyme digest using High Pure PCR Product Purification Kit.
- High fidelity PCR
- Site-directed mutagenesis
- Cloning
- Gene expression
- Study of allelic polymorphism in individual RNA transcripts
- Characterization of rare mutations in tissue
- Characterization of the allelic stage of single cells or single DNA molecules
Features and Benefits
Pwo SuperYield DNA Polymerase yields more high fidelity PCR product. The optimized buffer delivers superior results. Amplify fragments up to 3 kb. A GC-RICH Solution is included for difficult templates. Each dNTPack contains the 10 mM additive-free sodium salt nucleotides in a ready-to-use mix.
- Higher yield and 18-fold higher fidelity
- High performance with difficult templates.
- Reduce working steps in cloning.
- Cost-effective.
Packaging
1 kit containing 4 components
Quality
Each lot is assayed using activated DNA. PCR testing used λ and human genomic DNA.
Unit Definition
One unit Pwo SuperYield DNA Polymerase is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol total deoxynucleoside triphosphates into acid precipitable DNA within 30 minutes at +70 °C.
Unit Assay: Incubation buffer for assay on activated DNA
20 mM Tris-HCl, pH 8.8 (20 °C), 50 mM KCl, 2.5 mM MgCl2, 10 mM 2-mercaptoethanol, 0.2 mM of each dATP, dCTP, dGTP, dTTP.
Incubation procedure
12.5 mg activated calf thymus DNA and 0.1 mCi [-32P]dCTP are incubated with 0.01 to 0.1 U Pwo SuperYield DNA Polymerase in 50 µl incubation buffer with a paraffin oil overlay at +70 °C for 30 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation followed by scintillation counting.
Volume Activity: 5 U/µl
usage | sufficient for ≤40 reactions |
Quality Level | 100, |
packaging | pkg of 100 U |
Торговая марка | Roche |
parameter | 72 °C optimum reaction temp. |
shipped in | dry ice |
storage temp. | −20°C (−15°C to −25°C) |
hcodes | H412 |
pcodes | P273 - P501 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point F | does not flash |
Flash Point C | does not flash |
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа

С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии

Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.

У большинства компаний срок ожидания составляет 10-12 недель.

Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.

Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.

В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.

Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.