Proteases Roche
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Endoproteinase Asp-N Sequencing Grade
Enzyme Commission (EC) Number: 3.4.24.33 ( BRENDA | IUBMB )
Кат. номер 11420488001 11054589001 General descriptionThe wild type of Pseudomonas fragi segregates a metalloprotease, which cleaves peptide bonds N-terminally at small hydrophilic amino acids. A mutant of this strain produces the Endoproteinase Asp-N Sequencing Grade, which is isolated as a highly purified and specific protease.SpecificityEndoproteinase Asp-N Sequencing Grade is a metalloprotease. In phosphate, acetate, or Tris buffers at pH 6.0–8.5, it specifically cleaves peptide bonds N-terminally at aspartic and cysteic acid. If cysteine is reduced or alkylated, only --Asp-X is cleaved.
The specificity of Endoproteinase Asp-N Sequencing Grade is verified with glucagon as a substrate. High concentrations of Endoproteinase Asp-N Sequencing Grade (1 part by weight enzyme with 10 parts by weight glucagon) are incubated to detect traces of impurities like contaminating proteases.
Heat inactivation: The protease is stopped by boiling for 5 minutes.ApplicationUse Endoproteinase Asp-N Sequencing Grade for protein structure analysis and sequence analysis.Preparation NoteWorking concentration: 2 µg /50 µl double-dist. water
One vial (= 2 µg enzyme) is dissolved in 50 µl double-dist. water resulting in a buffer concentration of 10 mM Tris-HCl pH 7.5.
Working solution: Recommended solvent is double-distilled water.
Storage conditions (working solution): -15 to -25 °C
A solution in double-dist. water is stable for 1 week at maximum at 2 to 8 °C and for about 1 month at -15 to -25 °C; repeated thawing should be avoided.ReconstitutionLyophilized Endoproteinase Asp-N sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 10 mM Tris-HCl, pH 7.5. In order to avoid autolysis the incubation temperature should not exceed 37 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыassay 90% (SDS-PAGE) form lyophilized specific activity 20000 units/mg protein mol wt 27 kDa packaging pkg of 2 µg (11420488001), pkg of 3 x 2 µg (11054589001) Торговая марка Roche optimum pH 7.0-8.0 pH range 6.5 - 9.0 shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Endoproteinase Glu-C Sequencing Grade
Enzyme Commission (EC) Number: 3.4.21.19 ( BRENDA | IUBMB )
Кат. номер 11420399001 11047817001 General descriptionEndoproteinase Glu-C Sequencing Grade is a serine protease isolated from Staphylococcus aureus V8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, 2-macroglobulin and TLCK.SpecificitySerine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin Box) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin Box) are incubated to detect traces of impurities, for example, contaminating proteases.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.ApplicationUse Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.Features and BenefitsFree of impurities that may interfere with separation of peptides in RP HPLC.Preparation NoteWorking concentration: 1/100 to 1/20 (w/w)
With a minimum amount of 1 to 5 µg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 µm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): +2°C to +8°C for 1-2 days.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.ReconstitutionLyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform lyophilized (salt-free), lyophilized specific activity 15 units/mg protein mol wt Mr 27 kDa packaging pkg of 3 x 50 µg (11047817001), pkg of 50 µg (11420399001) Торговая марка Roche optimum pH 4.0-7.8 shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Endoproteinase Lys-C Sequencing Grade
Enzyme Commission (EC) Number: 3.4.21.50 ( BRENDA | IUBMB )
Кат. номер 11420429001 11047825001 General descriptionEndoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease.SpecificityThis enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0).
Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA.ApplicationUse Endoproteinase Lys-C Sequencing Grade for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.Preparation NoteStorage conditions (working solution): +2 to +8 °CReconstitutionLyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 µl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C.
Stable in 4 M urea.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform lyophilized specific activity 200 units/mg protein (at 25 °C, with Chromozym PL) mol wt 33 kDa by SDS-PAGE (reducing) packaging pkg of 3 x 5 µg (11047825001), pkg of 5 µg (11420429001) Торговая марка Roche optimum pH 8.5-8.8 pH range 5 - 12 shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Pepsin 10108057001
Enzyme Commission (EC) Number: 3.4.23.1 ( BRENDA | IUBMB ) General descriptionPepsin is an aspartic endoproteinase isolated from pig gastric mucosa. It is mainly used for the unspecific hydrolysis of proteins and peptides in acidic media. It also provides a limited hydrolysis of native immunoglobulins to yield biologically active fragments.SpecificityAspartic endopeptidase with relatively broad specificity. Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1′ positions.ApplicationPepsin has been used for the digestion of liver tissue obtained from rat.Preparation NoteWorking concentration: 0.05 to 0.5 mg/ml
Storage conditions (working solution): A pepsin solution is stable at 2 to 8 °C, at least for one week, at neutral pH and under germfree conditions. Frozen aliquots of the enzyme solution are expected to be more stable.
A stock solution in 10 mM HCl (5-10 mg/ml) should be stable for at least one week at 2 to 8 °C, too. Storage light protected, tightly closed.
A short (2 minutes) incubation at 40 °C has negligible effect on the activity of lyophilized pepsin or pepsin in solution.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform lyophilized (salt-free) Quality Level 100 specific activity ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.) packaging pkg of 1 g Торговая марка Roche optimum pH 1.8-2.2 shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H315 - H319 - H334 - H335 pcodes P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 Target Organs Respiratory system RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Trypsin Sequencing Grade, modified
Enzyme Commission (EC) Number: 3.4.21.4 ( BRENDA | IUBMB )
Кат. номер 11418033001 11418025001 General descriptionTrypsin Sequencing Grade, modified, is isolated from bovine pancreas as a highly purified and specific protease, and subsequently modified.
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, 2-macroglobulin,1-antitrypsin, APMSF, and antipain.SpecificityTrypsin is a serine endopeptidase. At pH 7.5–9, it specifically hydrolyzes proteins and peptide bonds C-terminally of Iysine and arginine. Amide and ester bonds of Arg and Lys are also cleaved. The specificity of Trypsin Sequencing Grade, modified, is verified with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Trypsin Sequencing Grade, modified, one part by weight enzyme with 9 parts by weight insulin Box, are incubated for 18 hours to detect traces ofchymotrypsin impurities.ApplicationUse Trypsin Sequencing Grade, modified, to generate glycopeptides from purified glycoproteins.
It is used for:- Protein-structure elucidation
- Tryptic mapping
- Fingerprinting analysis
- Sequence analysis
- Translocation studies
- Protein identification
QualityPurity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC.Preparation NoteWorking concentration: 1/100 to 1/5 of the protein by weight
Storage conditions (working solution): -15 to -25 °C
Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.
A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100 form lyophilized (salt-free) packaging pkg of 4 x 100 μg (11418033001), pkg of 4 x 25 μg (11418025001) Торговая марка Roche optimum pH 8.0 shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену
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