O-Glycosidase 11347101001

Enzyme Commission (EC) Number:

3.2.1.97 ( BRENDA | IUBMB )

O-Glycan-peptide hydrolase
O-Glycopeptide endo-D-galactosyl-N-acetyl--galactosamino hydrolase
O-Glycosidase is isolated from the culture filtrate of Diplococcus pneumoniae
Specificity
Hydrolyzes Gal-(1,3)GalNAc from O-glycans.
The enzyme releases the disaccharide Gal-(1,3) GaINAc from O-glycans, which is bound to serine or threonine as a core unit. Glycoproteins as well as glycopeptides can serve as substrates. Substitution of the disaccharide by sialic acid prevents cleavage.
O-Glycosidase is used to release the Gal-(1,3)-GaINAc unit from O-glycans (glycoproteins or glycopeptides). Bindings to serine as well as to threonine are hydrolyzed. Substituents at the disaccharide (e.g., sialic acid) prevent the hydrolysis and, therefore, have to be removed chemically or enzymatically (e.g., with neuraminidase from Arthrobacter or from Vibrio cholerae).
O-Glycosidase has been used to selectively deglycosylated chondroitin sulfate proteoglycans (CSPGs).
O-Glycosidase has been used in an experimental study done in order to perform comparative analysis of the fibulin protein family. It has also been used in deglycosylation studies, where protein extracts were incubated with O-Glycosidase and neuraminidase, during cell culture experiments.
Principle
Gal-β(1,3)-GaINAc - ↓ - (Ser/Thr) -peptide/protein (O-Glycosidase)
Unit Definition
One unit is the enzyme activity which releases 1 μM Gal-GaINAc from Gal-GaINAc-4-nitrophenyl within one minute at 37 °C at pH 5.
Physical form
Solution in 50 mM Na-phosphate, 0.02%, Micr-O-protect, 350 mM NaCI, pH 7.5, stabilized with BSA (bovine serum albumin [68 kD])
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
specific activity	>2.5 units/mg protein
mol wt	Mr ~160 kDa
packaging	pkg of 25 mU
Торговая марка	Roche
optimum pH	6.0-7.6
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash