Neuraminidase (Sialidase) 10269611001

Enzyme Commission (EC) Number:

3.2.1.18 ( BRENDA | IUBMB )

Neuraminidase hydrolyzes terminal N- or O-acylneuraminic acids which are 2,3-, 2,6-, or 2,8-linked (rate: 2,6 > 2,3 > 2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Specificity
Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,6 >α2,3 >α2,8, determined on bonds in tri- and tetrasaccharides.

• cell surface lectin array analysis.
• hemagglutination assays.
• cell adhesion assay.

For the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Neuraminidase has been used for the:

• detection of the cell surface glycosylations in human anaplastic large cell lymphoma cells
• release of sialic acid from cells
• antibody-overlay lectin microarray

Physical properties
This product is a mixture of isoenzymes (L, M1, M2 and S) with the following molecular weight values: ~ 52 kDa, 66 kDa and 88 kDa.
Physical form
Solution in 10 mM sodium phosphate, 0.1% Micr-O-Protect (w/v), 0.25 mg/ml bovine serum albumin, pH 7
Preparation Note
Working concentration: Enzyme/substrate ratio should be in the range of 0.04 U/25-80 µg.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
specific activity	~25 units/mg protein
packaging	pkg of 1 U (100 µl)
Торговая марка	Roche
optimum pH	5.0-5.5
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash