NBT 11585029001

Empirical Formula (Hill Notation):	C40H30Cl2N10O6
Linear Formula:	C40H30N10O6 · 2Cl
CAS Number:	298-83-9
Molecular Weight:	817.64
Beilstein/REAXYS Number:	4115923
MDL number:	MFCD00012159
PubChem Substance ID:	329749491

NBT is soluble in water, aqueous buffers, and dimethylformamide.
Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI. Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications. It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

• Colony and plaque hybridization
• Immunohistocytochemistry
• In situ hybridization
• Northern blot
• Southern blot
• Western blot

NBT has been used in in situ hybridization.
Quality
Performance-controlled in the LDH assay.
Preparation Note
Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.
Analysis Note
Absorption: NBT has its maximum absorption at 260 nm [in water],
and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
packaging	pkg of 5 g
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C
SMILES string	[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI	1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key	FSVCQIDHPKZJSO-UHFFFAOYSA-L

pictograms	GHS07
signalword	Warning
hcodes	H302 - H315 - H319 - H335
pcodes	P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
Flash Point F	does not flash
Flash Point C	does not flash