NADH 10107735001

Empirical Formula (Hill Notation):	C21H27N7Na2O14P2 · xH2O
CAS Number:	606-68-8
Molecular Weight:	709.40 (anhydrous basis)
MDL number:	MFCD03791273
PubChem Substance ID:	329748895

Nicotinamide adenine dinucleotide (NADH) has been used in:

• nicotinamide adenine dinucleotide (NAD+) /NADH cycling assay.
• adenosine triphosphate (ATP)ase assay.
• pyruvate kinase enzyme assay.

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
Biochem/physiol Actions
NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, -oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
Packaging
Packaged by solid weight.
Quality
Contaminants: Relative reaction with LDH: 1.00 ± 0.05 (referred to a standard)
Organic solvents: <4% (GC)
Preparation Note
Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C.
Analysis Note
Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at Hg 334 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at Hg 365 nm
For life science research only. Not for use in diagnostic procedures.
Shipping conditions may vary

Quality Level	100,
assay	100% (dry weight)
form	amorphous powder
mol wt	Mr 665.4 (NADH), Mr 709.4 (NADH-Na2)
packaging	pkg of 500 mg
Торговая марка	Roche
pH	7.5 (100 mg/mL in water, ±0.5), 7.5 ± 0.5 (100 mg/mL in water)
max	260 and 340 nm
shipped in	wet ice
storage temp.	2-8°C
SMILES string	O.[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O
InChI	1S/C21H29N7O14P2.2Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33;;;/h1,3-4,7-8,10-11,13-16,20-21,29-32H,2,5-6H2,(H2,23,33)(H,34,35)(H,36,37)(H2,22,24,25);;;1H2/q;2*+1;/p-2/t10-,11-,13-,14-,15-,16-,20-,21-;;;/m1.../s1
InChI key	FWBNCIFELNNJCX-UHOVGGJYSA-L

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable