Leupeptin

Empirical Formula (Hill Notation):	C20H38N6O4 · 0.5H2O4S
Linear Formula:	C20H38N6O4 · 1/2H2SO4
CAS Number:	103476-89-7
Molecular Weight:	475.59
MDL number:	MFCD00037012
PubChem Substance ID:	329817679

Кат. номер

11017101001

11529048001

11034626001

11017128001

Specificity
Leupeptin inhibits serine and thiol proteases such as trypsin, plasmin, proteinase K, kallikrein, papain, thrombin, and cathepsin A and B.
Not affected are -, -, - and - chymotrypsin, pepsin, cathepsin D, elastase, renin, and thermolysin.
Leupeptin as well as other protease inhibitors like antipain, chymostatin, pepstatin, and phosphoramidon are useful for the protection of proteins during their isolation from tissues or membranes. Leupeptin can be removed from the reaction by dialysis.
Note: To check other protease inhibitors, try our Protease Inhibitor Set including Antipain Dihydrochloride, Aprotinin, Bestatin, Chymostatin, E-64, EDTA-Na2, Leupeptin, Pefabloc SC, Pepstatin, and Phosphoramidon.
Biochem/physiol Actions
Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)
Features and Benefits
Contents
Synthetic, white powder
Quality
Function test: Performance controlled with trypsin.
Preparation Note
Working concentration: 0.5 to 5 µg/ml (1 to 20 µM)
Comparison of working concentrations of pefabloc with leupeptin and PMSF is in files.
Working solution: Solvent is recommended in distilled water.
Highly soluble in water (1 mg/ml), methanol, ethanol, acetic acid, dimethyl formamide and dimethyl sulfoxide.
Poorly soluble in acetone, chloroform, ethyl ether and n-hexane.
Storage conditions (working solution): -15 to -25 °C
In aqueous solution leupeptin is stable for 1 month at 2 to 8 °C or for at least 6 months at - 15 to -25 °C, stored under nitrogen. For best results, freeze the dissolved inhibitor in aliquots and avoid repeated thawing.
Reconstitution
Highly soluble in water (1 mg/ml). Stable for at least one week at 2 to 8 °C and 6 months frozen in aliquots at -15 to -25 °C.
Analysis Note
Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	synthetic
assay	96.5%
form	powder
mol wt	Mr 475.6
packaging	pkg of 100 mg (11529048001), pkg of 25 mg (11017128001), pkg of 5 mg (11017101001), pkg of 50 mg (11034626001)
Торговая марка	Roche
solubility	H2O: 50 mg/mL
shipped in	wet ice
storage temp.	2-8°C
SMILES string	OS(O)(=O)=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O
InChI	1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1
InChI key	CIPMKIHUGVGQTG-VFFZMTJFSA-N

RIDADR

NONH for all modes of transport