L-Malate Dehydrogenase (L-MDH)

Enzyme Commission (EC) Number:

1.1.1.37 ( BRENDA | IUBMB )

Кат. номер

10127256001

10127914001

10127248001

L-malate:NAD+ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.
The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.
Biochem/physiol Actions
The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.
Quality
Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Solution in 50% glycerol (v/v), pH approximately 7
Preparation Note
Activator: – phosphate
– arsenate
– Mg2+
– Zn2+
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution, suspension
specific activity	~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
packaging	pkg of 1 mL (10127248001 solution), pkg of 1 mL (10127256001 suspension), pkg of 5 mL (10127914001suspension)
Торговая марка	Roche
optimum pH	7.4-7.5(reduction of oxaloacetate), 9.2-9.5(oxidation of malate)
shipped in	wet ice
storage temp.	2-8°C

RIDADR

NONH for all modes of transport