KAPA Taq HotStart KK1512

NACRES:

NA.55

KAPA Taq is supplied in a 2X ReadyMix™ format containing all the components required for PCR except primers and template-simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye. KAPA Taq DNA polymerase is the single-subunit Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli.
KAPA Taq HotStart® may be used in:

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• Polymerase chain reaction (PCR)

Biochem/physiol Actions
KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 53 polymerase and 53 exonuclease activities, but no 3 5 exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. KAPA Taq hotStart buffer is a uniquely formulated buffer to facilitate specific primer annealing. This translates to higher yields of a specific product when compared to traditional Taq buffers, and improved amplification of GC- and AT-rich templates. However, KAPA Taq hotStart DNA polymerase may be used in combination with any standard Taq buffer with a pH of 8.3 or higher.
Features and Benefits
High performance

• Improved sensitivity, specificity, and yields.
• Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.

Quick Notes:

• KAPA Taq HotStart® DNA Polymerase can replace any commercial hotstart Taq DNA polymerase in an existing protocol. The final MgCl2 concentration and annealing temperature may need to be optimized to account for differences in formulation.
• The KAPA Taq HotStart Buffer is a uniquelyformulated buffer offering improved specificity and sensitivity, and improved amplification of GC- and AT-rich templates.
• The KAPA Taq HotStart Buffer does not contain MgCl2; MgCl2 (25 mM) is supplied separately to allow greater flexibility during reaction setup.
• The KAPA Taq HotStart PCR Kit is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Quality
Each batch of KAPA Taq HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq HotStart PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long term storage.
For Research Use Only. Not for use in diagnostic procedures.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Quality Level	100,
shelf life	18 mo.
feature	dNTPs included: no, hotstart
packaging	pkg of 2500 U
Торговая марка	Roche
application(s)	PCR: suitable
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash