KAPA Express Extract

NACRES:

NA.55

Кат. номер

KK7152

KK7151

KAPA Express Extract with polymerase chain reaction (PCR) ReadyMix™ is a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA in less than 15 minutes. DNA extractions are conveniently performed in a single-tube thereby greatly reducing the risk of sample loss and contamination.
The combination of KAPA Express Extract and KAPA2G Robust HotStart® ReadyMix™ provides a high performance solution for rapid DNA extraction and consistent downstream amplification.
KAPA Express Extract with PCR ReadyMix™ is ideally suited for the extraction of PCR-ready DNA from, but not limited to, the following sample types:

• Human tissue (formalin-fixed paraffin-embedded (FFPE) samples; blood collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples)
• Animal samples (ear or tail clippings; hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)
• Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples)
• Insects (crushed)
• Bird feathers (calamus fragments).

Features and Benefits
Key Features:

• Rapid extraction protocol: PCR-ready DNA in 15 minutes
• Versatility: single kit optimized for a variety of sample types
• Single-tube reaction minimizes risk of contamination
• DNA extracted with KAPA Express Extract is compatible with any PCR system

Rapid DNA extraction from a variety of sample types:

• From sample to PCR in less than 15 minutes
• Successful extraction from a variety of tissue types, including buccal swabs, hair follicles, fish tissue, and bird feathers

Increased PCR success from formalin-fixed paraffin-embedded (FFPE) samples or tissues:

• Rapid DNA extraction and consistent downstream amplification of challenging sample types

Routine DNA extraction from blood sample types:

• Efficient extraction from “Guthrie”cards, FTA® Elute cards, and FTA® cards

Quick Notes:

• Extract PCR-ready DNA from different tissue types in a quick and simple, single-tube protocol (15–20 min), without the need for hazardous chemicals or multiple washing steps.
• Significantly reduced turnaround times and risk of sample loss and contamination.
• Use 1 µL DNA extract per 25 µL PCR. Depending on the sample type, the extract may have to be diluted prior to PCR.
• One extract typically yields a sufficient amount of template for 50–500 PCRs.
• DNA extracts may be diluted in TE Buffer (1:5) or 10 mM Tris-HCl pH 8–8.5 (1:5–1:10) for long-term storage at -20°C. Extracts are stable at -20°C for 6 months.
• KAPA2G Robust HotStart® ReadyMix™ is recommended for consistent and reliable endpoint amplification of DNA extracts generated with KAPA Express Extract.
• No quantification of DNA required prior to PCR.

Quality
All components contained with KAPA Express Extract Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activities, and meet strict requirements with respect to DNA contamination.
Preparation Note
Handling:
KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.
For Research Use Only. Not for use in diagnostic procedures.
FTA is a registered trademark of Cytiva
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Quality Level	100,
usage	sufficient for 100 reactions, sufficient for 500 reactions
shelf life	≤12 mo.
packaging	pkg of 1000 U (KK7152), pkg of 200 U (KK7151)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07,GHS08
signalword	Warning
hcodes	H302 - H371
pcodes	P260 - P264 - P270 - P301 + P312 + P330 - P308 + P311 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash