- Производитель:
- Roche
Empirical Formula (Hill Notation): | C257H383N65O77S6 |
CAS Number: | 11061-68-0 |
Molecular Weight: | 5807.57 |
MDL number: | MFCD00131380 |
Specificity
Insulin, human, is active on most mammalian cells.
Biochem/physiol Actions
Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.
For life science research only. Not for use in diagnostic procedures.
Quality
Endotoxin level: <0.1EU/µg (LAL-test)
Note: 1 EU corresponds to 0.1ng
Sequence
A-chain: 21 AS, B-chain: 30 AS, connected by two disulfide bridges
Two polypeptide chains (A-chain: 21 amino acids, B-chain: 30 amino acids, connected by two disulfide bridges) identical to natural, human insulin.
Unit Definition
EC50 definition: The concentration of human insulin that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with 3T3 (A31) cells.
Physical form
Lyophilizate from a hydrochloric acid solution (pH 2.3) (crystalline insulin)
Preparation Note
Working concentration: 1-10 µg/ml
Recommended concentration for serum-free cell culture is 1-10 µg/ml.
The concentration of insulin required for stimulation of cell growth in almost all cases is extraordinarily high compared with the physiological concentration. Insulin may be mimicking insulin-like growth factors (IGFs, somatomedins) for some cell lines, and high insulin concentrations may be necessary to occupy receptors which have a high affinity for IGFs and a lower affinity for insulin.
Working solution: Dissolve Insulin, human, recombinant (100 mg or 500 mg), in sterile, double-dist. water (final concentration: 10 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The reconstituted, undiluted solution is stable at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Reconstitution
Reconstitute in sterile double-distilled water (final concentration 10 mg/ml), further dilution with PBS (phosphate buffered saline) or medium containing 1 mg/ml (0.1%) BSA (bovine serum albumin) [or HSA (human serum albumin)], or 1 to 10% serum.
Quality Level | 100 |
sterility | non-sterile; 0.2 µm filtered |
assay | >98% (SDS-PAGE) |
form | lyophilized (clear, colorless solution after reconstitution) |
specific activity | >26 units/mg protein (At least the same specific activity (EC<sub>50</sub>) compared to the indicated standard is guaranteed.) |
mol wt | 5,700 Da |
packaging | pkg of 100 mg |
Торговая марка | Roche |
application(s) | cell culture | mammalian: suitable |
impurities | <0.1 EU/µg endotoxin (LAL test) |
solubility | water: soluble |
UniProt accession no. | P01308, |
shipped in | wet ice |
storage temp. | 2-8°C |
InChI key | PBGKTOXHQIOBKM-FHFVDXKLSA-N |
Gene Information | human ... INS(3630) |
RIDADR | NONH for all modes of transport |
WGK Germany | nwg |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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