Immunoprecipitation Kit (Protein G) 11719386001

Kit contains all reagents necessary for cell lysis, solubilization, stabilization, and immunopurification of proteins.
Specificity
Protein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins.
The immunoprecipitation kit has been used for the immunoprecipitation of proteins from cellular extracts with Protein G Agarose.
Packaging
1 kit containing 5 components.
Preparation Note
Working solution: Lysis buffer/wash buffer 1
The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete Tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.
Wash buffer 2
The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Wash buffer 3
The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 20 reactions
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS09
signalword	Danger
hcodes	H314 - H317 - H411
pcodes	P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P391
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash