GTP 10106399001

Empirical Formula (Hill Notation):	C10H16N5O14P3 · xNa+ · yH2O
CAS Number:	36051-31-7
Molecular Weight:	523.18 (anhydrous free acid basis)
Beilstein/REAXYS Number:	4113439
MDL number:	MFCD00149227
PubChem Substance ID:	329748894

GTP is supplied as the disodium salt in crystal form.
GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.
GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.
Quality
Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na+, 8% H2O
Preparation Note
Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.
If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.
Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	>90% (HPLC)
mol wt	Mr 523.2 (GTP), Mr 567.1 (GTP-Na2)
packaging	pkg of 250 mg
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
shipped in	wet ice
SMILES string	O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
InChI	1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1
InChI key	HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable