Endoproteinase Arg-C Sequencing Grade 11370529001

Enzyme Commission (EC) Number:

3.4.22.8 ( BRENDA | IUBMB )

Endoproteinase Arg-C Sequencing Grade is isolated from Clostridium histolyticum as a highly purified and specific cysteine protease.
Specificity
Endoproteinase Arg-C Sequencing Grade is a cysteine protease that specifically cleaves peptide, ester, and amide bonds at the C-terminal side of arginine residues. The specificity is primarily to arginine residues, although hydrolysis proceeds to a minor degree in most lysine-containing substrates. Reducing agents (such as DTT) and Ca2+ are required for full activity.
The specificity of Endoproteinase Arg-C Sequencing Grade is tested with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Endoproteinase Arg-C Sequencing Grade are incubated for prolonged incubation times to detect traces of impurities such as contaminating proteases.
Recognition sites: C-terminal side of arginine residues, other recognition sites with lower efficiency.
Use Endoproteinase Arg-C Sequencing Grade for the specific cleavage of proteins and peptides for peptide mapping, fingerprinting, and sequence analysis. The protease is suitable to digest proteins in solution, in gels, or on blotting membranes.
Quality
Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.
Preparation Note
Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and -mercaptoethanol.
Inhibitors: Oxidizing agents, sulfhydryl reactants (e.g., TLCK), EDTA, Co2+, Cu2+, Cd2+. Citrate, borate, and Tris anions partially inhibit.
Optimum pH: 7.2 - 8.0
Storage conditions (working solution): -15 to -25°C
For long-term use, the solution should be gently flushed with a stream of nitrogen and stored at -15 to -25°C. The reconstituted incubation buffer is stable for several months at -15 to -25°C.
Reconstitution
The lyophilized endoproteinase Arg-C sequencing grade is reconstituted in 50 µl double-dist. water to give a final concentration of 50 mM Tris-HCl buffer, 10 mM CaCl2, 5 mM EDTA, pH 8.0. The lyophilized activation solution is dissolved in 100 µl double-dist. water resulting in a final 50 mM dithiothreitol-(DTT-)concentration and 5 mM EDTA-concentration.
For life science research only. Not for use in diagnostic procedures.

form	lyophilized
mol wt	Mr 59 kDa
packaging	pkg of 3 x 5 µg
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
optimum pH	7.2-8.0
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H302 - H315 - H318 - H334
pcodes	P261 - P264 - P280 - P304 + P340 - P305 + P351 + P338 + P310 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash