DNA Molecular Weight Marker XVII (500 bp ladder) 11855646001

Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 0.5 to 5.0 kbp.
DNA Molecular Weight Marker XVII (500 bp ladder) allows accurate sizing of DNA fragments generated by PCR, RT-PCR or restriction digest, following separation on agarose gels. The DNA Molecular Weight Marker XVII can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder), DNA Molecular Weight Marker XIV (100 bp ladder), and DNA Molecular Weight Marker XVI (250 bp ladder) for precise size determination.
Sequence
The mixture contains 10 blunt-ended, double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, and 5000. The 2000- and 4000-bp banding pattern are three times brighter. Electrophoretic separation of this molecular weight marker results in a regular pattern.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The fragments exhibit uniform spacing for easier reading, while bands 2000 and 4000 are 3 times brighter for easy orientation.
Unit Definition
50 µg = 1A260 unit
Physical form
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed, store at 2 to 8 °C.
For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 50 μg (in 200 μl)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash