Collagenase P

Enzyme Commission (EC) Number:

3.4.24.3 ( BRENDA | IUBMB )

Кат. номер

11213857001

11249002001

11213865001

11213873001

Collagenase P is an enzyme mixture prepared from the extracellular culture filtrate of a special Clostridium histolyticum strain.Collagenase P is a research biochemical, not manufactured under the US good manufacturing practices (GMP) regulations.
Note: Collagenase P is a non-sterile preparation of the culture supernatant of Clostridium histolyticum. It is neither designed nor intended for isolation of pancreatic islets for transplantation into humans and must not be used for such purposes.
Enzyme activity:
Collagenase activity: >1.5U/mg (according to Wunsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase P has a very high collagenase activity (>1.5U/mg), and is function tested for the isolation of mouse pancreatic islets.
Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.
Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Because of its high collagenase activity Collagenase P is particularly suitable for the isolation of pancreatic islets from mouse and rat (function tested). The preparation is also suitable for the isolation of adipocytes from epididymal fat pads of rats.
Contents:
Lyophilizate, nonsterile
Activator: Ca2+
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Working concentration: 0.5 to 2.5mg/ml
0.5 to 1.5mg/ml for the isolation of pancreatic islets from mouse and rat pancreas.
Approximately 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C. Prepare appropriate aliquots and avoid repeated freezing and thawing.
Reconstitution in any balanced salt solution (e.g., HBSS)
For life science research only. Not for use in diagnostic procedures.

Quality Level	100
sterility	non-sterile
form	lyophilized
packaging	pkg of 1 g (11249002001), pkg of 100 mg (11213857001), pkg of 2.5 g (11213873001), pkg of 500 mg (11213865001)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR

NONH for all modes of transport