Collagen 11179179001

Collagens are abundantly present in vertebrates. 41 specific genes codes for 42 polypeptide chains and produce 27 collagen types. Collagens are mainly present in the extracellular matrix. Collagen type I is abundantly present in mammals. Type IV collagen is an important structural protein of basement membranes. It is chemically and genetically different from stroma collagen types I and III and cartilage collagen type II.
Specificity
Active on most mammalian cells.
Collagen is used as a substrate for culturing a variety of cells, such as coating of culture dishes and the preparation of collagen gels.
Collagen has been used in the preparation of coverslip coating solution and collagen gel contraction assay.
Biochem/physiol Actions
Collagen provides extracellular support for multicellular animals. Collagen type I offers mechanical stability, strength and toughness to a range of tissues from tendons and ligaments, to skin, cornea, bone and dentin.
Features and Benefits
Contents
Lyophilizate, cell culture grade, 3 vials of 10mg
Specifications
Biological activity: Tested for the promotion of adherence of HUV-EC cells.
Preparation Note
Working concentration: 5 µg/cm2
5 µg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.
Reconstitution
IFor best results, dissolve the lyophilizate in 0.2% acetic acid (v/v) solution.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.
Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.
For life science research only. Not for use in diagnostic procedures.

biological source	rat tail
Quality Level	100
sterility	sterile
form	lyophilized (clear, colorless solution after reconstitution)
packaging	pkg of 30 mg
Торговая марка	Roche
application(s)	cell culture | mammalian: suitable
UniProt accession no.	P02454,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	rat ... Col1a1(29393)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash