Cell Dissociation Reagents Roche
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Trypsin Inhibitor 10109878001
General descriptionEgg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).ApplicationTrypsin Inhibitor has been used:- as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting
- to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture
- to neutralize trypsin post dissociation and trypsinization of brain tissues
- termination of tissue disaggregation and for subcultivation procedures
Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.Preparation NoteWorking concentration: 0
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.ReconstitutionBoth are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.Storage and StabilityStore at 2 to 8 °C. (Store dry!)Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыbiological source chicken egg white Quality Level 100, form powder packaging pkg of 1 g Торговая марка Roche concentration 1 mg/mL application(s) cell culture | mammalian: suitable optimum pH 3.8-4.5 solubility water: soluble shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Trypsin Inhibitor 10109886001
General descriptionSoybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.SpecificitySoybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).ApplicationTrypsin Inhibitor has been used:- in the buffer for hepatocyte isolation
- to inhibit trypsin in Corneal epithelial cells
- to inhibit papain activity while treating hippocampal neural stem cells (NSCs)
Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.Biochem/physiol ActionsSoybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.SpecificationsInhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)
Isoelectric point: 4.5Unit Definition1 U = 270 S & T-units or 90 USP-unitsPreparation NoteWorking concentration: 10 to 100 µg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.ReconstitutionSensitive to heat, alkaline pH, and protein-precipitating compounds.Storage and StabilityStore at 2 to 8 °C. (Store dry!)Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыbiological source soybean Quality Level 100 form lyophilized mol wt Mr 20,100 packaging pkg of 50 mg Торговая марка Roche concentration 1 mg/mL application(s) cell culture | mammalian: suitable pH 3.8-4.5 solubility water: soluble shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point(F) Not applicable Flash Point(C) Not applicable Узнать цену
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