Calpain Inhibitor I 11086090001

Empirical Formula (Hill Notation):	C20H37N3O4
CAS Number:	110044-82-1
Molecular Weight:	383.53
Beilstein/REAXYS Number:	7656053
MDL number:	MFCD00065505
PubChem Substance ID:	329749147

Calpain Inhibitor I is a strong, competitive inhibitor of the Ca2+-dependent neutral cysteine proteases calpain I and calpain II.
Specificity
Calpain Inhibitor I is a strong, competitive inhibitor of the Ca2+-dependent neutral cysteine proteases calpain I (requiring 2–75 µM Ca2+ for activation) and calpain II (requiring 200–800 µM) with a Ki-value between 0.12 µM and 0.23 µM. Calpain II is inhibited to a lesser extent than calpain I. Calpain Inhibitor I is also a strong inhibitor of papain and cathepsin B and L. It shows only weak inhibition of cathepsin H and -chymotrypsin and does not inhibit trypsin. ID50 for 0.02 U platelet calpain is 0.05 µM.
Calpain inhibitor I inhibits calpain, which activates myosin light chain kinase and protein kinase C by partial proteolysis. The inhibitor should be membrane permeable due to low molecular weight and lack of charged residues.
Calpain Inhibitor I is used for western blotting methods.
Quality
Purity: 98% (from C); chromatographically homogeneous
Formula variant
C20H37N3O4
Preparation Note
Working concentration: 0.1 - 10 µM
The suggested starting concentration is 17 µg/ml. This is the concentration at which half maximal inhibition of calpain I is observed.
Working solution: Recommended solvent is DMF, ethanol, or methanol up to 10 mg/ml.
Storage conditions (working solution): -15 to -25 °C
Solutions in DMF, methanol, ethanol are stable for 4 weeks at -15 to -25 °C.
Reconstitution
Soluble in DMF, ethanol, or methanol to 10 mg/ml. For a stock solution, Roche recommends dissolving 1 mg of the inhibitor in 100 μl DMF, methanol, or ethanol. Before use, dilute with water or phosphate buffer (0.1 M, pH 7.5) to desired concentration. Solutions in DMF, ethanol, or methanol are stable for 2 to 3 days at 2 to 8 °C, and approximately 4 weeks at -15 to -25 °C. For best results, prepare solutions fresh before use.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	98% (chromatographically homogeneous)
form	powder
mol wt	Mr = 383.5
packaging	pkg of 25 mg
Торговая марка	Roche
mp	182 °C
solubility	DMF: 10 mg/mL, ethanol: 10 mg/mL, methanol: 10 mg/mL
shipped in	wet ice
storage temp.	2-8°C
SMILES string	[H]C(=O)[C@H](CCCC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O
InChI	1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1
InChI key	FMYKJLXRRQTBOR-BZSNNMDCSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable