Attachment Factors Roche
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Fibronectin 10838039001
MDL number: MFCD00131062 General descriptionFibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.
Specificity
Active on most mammalian cellsApplicationFibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.
Testing Method
Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)
Physical form
Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.
Preparation Note
Working concentration: 5 µg/cm2
5 µg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Reconstitution
Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100 biological source human plasma sterility non-sterile; 0.2 µm filtered assay 95% (SDS-PAGE) form lyophilized (clear, colorless solution after reconstitution) mol wt 440 kDa packaging pkg of 5 mg Торговая марка Roche storage condition avoid repeated freeze/thaw cycles application(s) cell culture | mammalian: suitable UniProt accession no. P02751, shipped in wet ice storage temp. 2-8°C Gene Information human ... FN1(2335)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F does not flash Flash Point C does not flash Узнать цену -
Laminin 11243217001
CAS Number: 114956-81-9 MDL number: MFCD00081739 General descriptionLaminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from , and chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.
Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.ApplicationLaminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 µg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Quality
Purity: 90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)
Sequence
Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.
Physical form
Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane
Preparation Note
Working concentration: 2 to 5 µg/cm2
2 to 5 µg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100 biological source mouse (Engelbreth-Holm-Swarm (EHS) sarcoma) sterility sterile; sterile-filtered assay 90% (SDS-PAGE) form solution mol wt 900 kDa packaging pkg of 1 mg (2 ml) Торговая марка Roche application(s) cell culture | mammalian: suitable UniProt accession no. Q61292, shipped in dry ice storage temp. 20°C Gene Information mouse ... Lamb2(16779)
Safety InformationRIDADR NONH for all modes of transport WGK Germany nwg Flash Point F does not flash Flash Point C does not flash Узнать цену
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