Antibody Protein Purification Roche
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Protein A Agarose
Кат. номер 5015979001 11719408001 11134515001 General descriptionThe use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic, and are contaminants in Protein A isolated from S. aureus. The immobilization chemistry provides high IgG-binding capacity and leak-proof stability under a wide range of pH and buffer conditions. The gel can be used over 30 times, depending on the elution condition.
Protein A leakage: <18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98% pure, HPLC, SDS-Page)/1 ml gel
Protein A, immobilized.SpecificityProtein A is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein A binds to varying degrees IgM, IgA, IgD, and most subclasses of IgG.ApplicationPurification of mouse monoclonal antibodies from ascites fluid or cell culture supernatants. Protein A or Protein G Agarose affinity matrix can be used to purify antibodies from crude cell extracts and for the immunoprecipitation or co-immunoprecipitation of proteins. It has also been used for photoactivatable-ribonucleoside-enhanced cross-linking immunoprecipitation (PAR-CLIP) analysis.ComponentsPreswollen gel, ready-to-use, 2 ml or 5 ml gel (settled gel volume) in buffer that contains 20% ethanol as a preservative, nonsterile.
Protein A content: 3 mg/ml pre-swollen gelQualityPurity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыassay >98% (HPLC and SDS-PAGE) form suspension packaging pkg of 15 mL (05015979001 [settled resin volume]), pkg of 2 mL (11719408001 [settled resin volume]), pkg of 5 mL (11134515001 [settled resin volume]) Торговая марка Roche capacity >18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0) shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Protein G Agarose
Кат. номер 5015952001 11719416001 11243233001 General descriptionProtein G was initially isolated from G148, a human group G Streptococcal strain. It is a cell wall protein and shows high affinity to IgG (immunoglobulin G). It has a putative molecular weight of 30,000Da. It interacts with all subclasses of human IgG and also binds to mouse, rabbit and goat IgG.SpecificityProtein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins.
Binding capacity: 20mg human IgG (polyclonal)/ml (the IgG is loaded at pH 7.0, and eluted with 200mM glycine, pH 2.8)
Structure: recombinant Protein G (E. coli, Mr = 22,000) is covalently coupled to crosslinked 4% agarose beads: 2.5mg Protein G (>98% pure)/1ml gel.ApplicationAffinity chromatography using Protein G Agarose, like Protein A Agarose, is the method of choice for purification of IgG from many species. It has been used as a pre-clearing agent during-- Ubiquitination assay
- Immunoprecipitation
Features and BenefitsContents
Preswollen gel, ready-to-use, 2ml or 5ml gel (settled gel volume) in buffer that contains 14-19% ethanol as a preservative, nonsterile.
Protein G content: 2.5mg/ml preswollen gelQualityPurity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыassay >98% (HPLC and SDS-PAGE) form suspension packaging pkg of 15 mL (05015952001 [settled resin volume]), pkg of 2 mL (11719416001 [settled resin volume]), pkg of 5 mL (11243233001 [settled resin volume]) Торговая марка Roche shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену
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