Anti-Digoxigenin 11333089001

Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.
Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.
Specificity
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:

• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Western blot

Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.
Biochem/physiol Actions
In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.
Preparation Note
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:

• ELISA: for coating: 2 to 4 µg/ml
• Immunohistocytochemistry: 0.5 to 2 µg/ml
• In situ hybridization: 0.2 to 0.4 µg/ml
• Western blot: 0.5 to 2 µg/ml

Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.
Reconstitution
Add 1 ml PBS to a final concentration of 200 μg/ml.
Analysis Note
No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.
For life science research only. Not for use in diagnostic procedures.

biological source	sheep
Quality Level	100,
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	polyclonal
form	lyophilized
packaging	pkg of 200 μg
Торговая марка	Roche
isotype	IgG
conjugate	unconjugated
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash