Albumins & Transport Proteins Roche
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2-Macroglobulin 10602442001
General descriptionα2-Macroglobulin belongs to the macroglobulin superfamily. It is a high molecular weight tetrameric glycoprotein with receptor-binding domain (RBD), thioester-containing domain (TED) domain and C1r/C1s, Uegf, Bmp1 (CUB) domain.Applicationα2-Macroglobulin has been used as a component of homogenization buffer for processing hemibrains of young mice.
Biochem/physiol Actions
α2-Macroglobulin is a plasma endopeptidase and glycoprotein which functions on proteinases, especially serine proteases. It interacts with and inhibits endopeptidases. However, it does not act on other proteases, including inactive proteinases.
α2-Macroglobulin (α2M) binds several hormones and may regulate their activity. α2M also aids protection against various infections.
Sequence
2-macroglobulin, a glycoprotein, is a tetramer of identical subunits, which are Iinked in pairs by disulfide bonds.
Preparation Note
Working solution: Recommended solvent is distilled water.
Storage conditions (working solution): -15 to -25 °C
Reconstitution
Soluble in water. Stable for at least one week at room temperature (15 to 25 °C), or 3 weeks at 2 to 8 °C. Can also be frozen in aliquots at -15 to -25 °C, where it remains stable for at least 6 months. Sensitive to acidic pH, denatured below pH 4.0. Ammonia methylamine and hydroxylamine (above pH 7.0) cause irreversible conversion to the inactive form.
Note: Do not use 2-macroglobulin in the presence of DTT since it causes a (reversible) dissociation into inactive subunits. 2-macroglobulin acts by physically entrapping endoproteinases, usually in a 1:1 ratio.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)
Analysis Note
Specific activity: approximately 1 inhibitor U/mg protein (tested with trypsin at +25°C and Chromozym TRY as substrate).
Crossreactivity with pregnancy associated glycoproteins: deglycosylation of 2-macroglobulin is recommended.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыbiological source bovine plasma Quality Level 100 form lyophilized (Contains 6% BSA) packaging pkg of 25 IU Торговая марка Roche application(s) blocking: suitable pH range 4 - 7 UniProt accession no. Q7SIH1, shipped in wet ice Gene Information bovine ... A2M(513856)
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Bovine Serum Albumin 10711454001
General descriptionThe product, bovine serum albumin, is of special quality for molecular biology. Bovine serum albumin has homology with human serum albumin. Serum albumins are soluble proteins of the circulatory system and participate in various cellular processes.
Contents
Solution of 20mg/ml Bovine Serum Albumin in 50mM Tris-HCl, 0.1M NaCl, 0.25mM EDTA, 1mM 2-mercaptoethanol, 50% glycerol (v/v), pH approximately 7.6.ApplicationBovine Serum Albumin supports enzyme stability. It has been used for the:- preparation of circular DNA molecules
- amplification of bacterial 16S rRNA genes
- BOX-PCR fingerprinting
QualityContaminants: Albumin is checked for the absence of DNases, nicking activity, RNases, and proteases according to the current Quality Control procedures.Physical formSolution of 20 mg/ml Bovine Serum Albumin in 50 mM Tris-HCl, 0.1 M NaCl, 0.25 mM EDTA, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), pH approx. 7.6.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, biological source bovine form solution packaging pkg of 1 mL (20 mg/ml) Торговая марка Roche pH 4.8-7.5 shipped in dry ice storage temp. −20°C (−15°C to −25°C)
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Bovine Serum Albumin Fraction V, fatty acid free 10775835001
General descriptionHighest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.ApplicationBovine Serum Albumin, Fraction V, fatty acid free is used:- in lipid research to study lipid metabolism in biological systems
- to identify transport mechanisms in living organisms
- to study ligand binding
- to investigate ionic binding
- in the preparation of solutions during isolation of cardiomyocytes
- as a blocking solution to incubate embryos for immunofluorescence
- in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers
Biochem/physiol Actions
The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.
Features and Benefits- Free from fatty acids according to current quality control procedures (suitable for lipid research)
- High quality
- Low content of heavy metal ions
- Long-term stability
Quality
Purity: 98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), 5% H2O,30 ppm heavy metalsOther NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100 biological source bovine assay 98.5% (electrophoresis) form lyophilized mol wt Mr 68 kDa packaging pkg of 50 g Торговая марка Roche application(s) immunofluorescence: suitable","ligand binding assay: suitable UniProt accession no. P02769, shipped in wet ice storage temp. 2-8°C Gene Information bovine ... ALB(280717)
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Bovine Serum Albumin Fraction V, heat shock, fatty acid free 3117057001
General descriptionBSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.Application- Pharmacological assays; ion-binding, transport, or immunological studies
- Fatty acid and lipid research
- Stabilization of proteins and enzymes against proteolysis and denaturation
Biochem/physiol ActionsThe fatty acids that are insoluble in circulating plasma are transported primarily by serum. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.QualityPurity: 98% albumin (electrophoresis), 98% protein
Contaminants: 1.2mg total fatty acids/g dry powder, 30ppm heavy metals, immunoglobulins are not detectable (by Ouchterlony)SpecificationsMoisture: 5%Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, biological source bovine assay 98% form lyophilized packaging pkg of 100 g Торговая марка Roche pH ~7.0 shipped in wet ice storage temp. 2-8°C
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Bovine Serum Albumin Fraction V, protease-free 3117332001
ApplicationBovine Serum Albumin Fraction V, protease-free is used:- for stabilization of purified enzymes
- for site-blocking reagent in ELISA techniques
- as a protein standard for determination of protein concentration
QualityContaminants: 0.001% heavy metals; proteases not detectable (casein digest)Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, biological source bovine assay ≥=98.5% albumin basis (electrophoresis) form lyophilized packaging pkg of 50 g Торговая марка Roche shipped in wet ice storage temp. 2-8°C
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Plasmin, bovine 10602370001
Enzyme Commission (EC) Number: 3.4.21.7 ( BRENDA | IUBMB ) General descriptionPlasmin is derived from the proteolytic cleavage of its precursor plasminogen. Human Plasmin is made up of two polypeptide chains. The polypeptide chains are connected via disulfide bridges.SpecificitySerine endopeptidase that hydrolyzes peptide and ester bonds at the carboxylic side of Lys or Arg; more selective than trypsin.ApplicationPlasmin from bovine has been used as a standard for assessing plasmin biosensors and for proteolytic assays.Biochem/physiol ActionsPlasmin plays an important role in proteolysis and fibrinolysis. It possesses inflammatory activity. Plasmin is also involved in the hydrolysis of peptide bonds following arginine or lysine. It also cleaves hormones like glucagon, thrombopoietin and growth hormones. Plasmin plays a role in cleavage of complement factors like C3 and C5, coagulation factors V and VIII, matrix proteins, growth factors, E-cadherins and metalloproteinase.Physical formSuspension in 3.2 M ammonium sulfate solutionOther NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, form suspension specific activity 2 units/mg protein (At 25 °C with Chromozym PL as the substrate.) mol wt Mr ~85 kDa packaging pkg of 1 mL (5 U) Торговая марка Roche optimum pH 8.9 shipped in wet ice storage temp. 2-8°C
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Plasmin, human 10602361001
Enzyme Commission (EC) Number: 3.4.21.7 ( BRENDA | IUBMB ) General descriptionFibrinolysin
Inhibitors: DFP, PMSF, TLCK, leupeptin, aprotinin, soybean trypsin inhibitor, 1-antitrypsin, 2-macroglobulin, anti-thrombin III heparin, APMSF, and 2-antiplasminSpecificitySerine endopeptidase that hydrolyzes peptide and ester bonds at the carboxylic side of Lys or Arg; more selective than trypsin.ApplicationHuman plasmin has been used as an oligopeptide substrate in peptidase inhibition assays.Biochem/physiol ActionsPlasmin is a direct acting fibrinolytic enzyme present in blood. Its activity is independent of plasminogen presence during fibrinolysis. Plasmin is rapidly and irreversibly inhibited by α-2 antiplasmin.Physical formSuspension in 3.2 M ammonium sulfate solutionPreparation NoteStabilizers: Human plasmin does not contain stabilizers.
Storage conditions (working solution): 2 to 8 °C
Diluted solution (pH 8.6) is stable at 2 to 8 °C for at least 1–2 weeks. For storage of dialyzed solution freezing in aliquots is recommended.Storage and StabilityStore at 2 to 8 °C. (Recommended form for storage is ammonium sulfate suspension 3.2 M, pH 8.6.)Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, assay 95% (electrophoresis) form suspension specific activity ~8 units/mg protein (At 25 °C with Chromozym PL as the substrate.) mol wt Mr ~85 kDa packaging pkg of 0.5 mL (5 U) Торговая марка Roche optimum pH 8.9 shipped in wet ice
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Plasminogen 10874477001
General descriptionPlasminogen is a profibrinolysin from human serum. Plasminogen, the proenzyme of the fibrinolytically active enzyme plasmin, is a single chain glycoprotein. Various isoelectric forms exist and can be separated by means of isoelectric focusing (IEF). The lyophilizate is stabilized with bovine serum albumin (BSA).ApplicationPlasminogen is used for the determination of the activity of plasminogen activators. The enzyme is a substrate for tissue plasminogen activator (t-PA) in the t-PA test and a reference substance for t-PA therapy. It is used as the in-process control in the production of t-PA.Features and BenefitsContents
Lyophilizate, stabilized with bovine serum albumin.QualityContaminants: <0.05% plasmin; the preparation does not contain 6-aminohexanoic acidSequenceNative plasminogen (glu-plasminogen) has a N-terminal glutamic acid group. Partial plasma proteolysis gives rise to a molecule with N-terminal lysine (lys-plasminogen). Cleavage with elastase results in val-plasminogen. The complete amino acid sequence contains 790 amino acids. Roche Applied Science supplies glu-plasminogen.Preparation NoteStabilizers: stabilized with BSA
Working solution: Solvent is recommended in 20 mM sodium phosphate buffer, pH 7.5, 100 mM NaCl.
Storage conditions (working solution): -15 to -25 °C
A solution in 20 mM sodium phosphate buffer, pH ca. 7.5, 100 mM NaCl is stable for several months without loss of activity when stored at -15 to -25 °C.
Note:
Plasminogen is susceptible to autolysis, which may result in activation. Contact with the skin or other contamination (e.g., by microorganisms), is therefore to be avoided.Storage and StabilityStore at 2 to 8 °C. (Store dry!)Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, form lyophilized specific activity ~11 units/mg protein (activated with streptokinase at 37 °C and pH 7.4 with Chromozym PL as the substrate.) mol wt 90-94 kDa packaging pkg of 20 U Торговая марка Roche shipped in wet ice
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Thrombin 10602400001
Enzyme Commission (EC) Number: 3.4.21.5 ( BRENDA | IUBMB ) General descriptionThrombin is a Na+ activated allosteric serine protease. It belongs to chymotrypsin family. Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond.
Thrombin is a coagulation factor IIa. It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg. The enzyme converts fibrinogen to fibrin. The product contains EDTA and additional stabilizing agents.ApplicationThrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.
Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members. It has also been used in platelet spreading on fibrinogen and immunostaining.Biochem/physiol ActionsThrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C.Physical formLyophilizatePreparation NoteStorage conditions (working solution): -15 to -25°C
Inhibitors: DFP, TLCK, PMSF, benzamidine, 1-antitrypsin, 2-macroglobulin, antithrombin III heparin, hirudin, and APMSFReconstitutionThe reconstituted solution contains 20 mM EDTA.
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!Storage and StabilityStore at 2 to 8 °C. (Store dry!)Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, form lyophilized specific activity ~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.) mol wt Mr ~33.6 kDa packaging pkg of 20 U Торговая марка Roche optimum pH 8.2-9.0 shipped in wet ice
Safety Informationpictograms GHS07 signalword Warning hcodes H332 pcodes P261 - P271 - P304 + P340 + P312 RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F does not flash Flash Point C does not flash Узнать цену
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