-Gal Reporter Gene Assay, chemiluminescent 11758241001

The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid.
The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene.
Features and Benefits

• Sensitive: Chemiluminescence technology allows detection of approximately 20 fg -galactosidase in cell extracts
• High dynamic measuring range: Linear range over four to five orders of magnitude
• Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
• Safe: No radioactive isotopes are used
• Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
• Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control

Packaging
1 kit containing 7 components.
Preparation Note
Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 250 assays (tubes), sufficient for 500 assays (microplate)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS02,GHS05,GHS07
signalword	Danger
hcodes	H225 - H290 - H314 - H317 - H336 - H412
pcodes	P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378
Target Organs	Central nervous system
RIDADR	UN 3316 9
WGK Germany	WGK 2