Recombinant/Fusion Tag Protein Purification Millipore
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Anti-HA Immunoprecipitation Kit IP0010-1KT
NACRES: NA.56 ApplicationThe Anti-HA Immunoprecipitation Kit is specially designed to allow maximal recovery of HA-tagged proteins in the immunoprecipitates. The whole process is performed in mini-spin columns, instead of in microcentrifuge tubes, thus combining convenient washing of the antigen-antibody-bound beads with minimal variability between experiments.Features and Benefits- Spin columns ensures more consistent results
- Anti-HA Affinity Gel ensures high specific binding to HA-tagged fusion protein
- CelLytic M Cell Lysis Reagent for rapid and efficient mammalian cell lysis
ПараметрыQuality Level 200 antibody product type primary antibodies clone monoclonal analyte chemical class(es) proteins (hemagglutinin) application(s) immunoprecipitation (IP): suitable storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EZview™ Red Anti-c-Myc Affinity Gel
NACRES: NA.56
Кат. номер E6654-5X1ML E6654-1ML General descriptionEZ view Red Anti--c-Myc Affinity Gel is a resin that consists of the Anti-c-Myc rabbit antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind c-Myc fusion proteins to samples, such as cell lysates and tissue, for purification of c-Myc-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
Agarose beads bind at N-terminal, Met-N-terminal and C-terminal c-Myc fusion proteins from E. coli and mammalian cells.ApplicationEZview® Red Anti-c-Myc Affinity Gel is suitable for:- SUMOylation assay
- Affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from E. coli or mammalian cells.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.Packaging1, 5×1 mL in poly bottleFeatures and Benefits- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Other NotesThe affinity gel contains affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to beaded agarose. The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein.Physical form1:1 (v/v) suspension in PBS containing 50% glycerol and 0.0015% Kathon CG/IPCII as an antimicrobial preservativeLegal InformationEZview is a trademark of Sigma-Aldrich Co. LLC
SabreLite is a registered trademark of Pelican Products, Inc.Параметрыshelf life 1 yr at 28 °C Quality Level 200 analyte chemical class(es) proteins (c-Myc) application(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable capacity ~0.05 mg/mL binding capacity storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EZview™ Red Anti-HA Affinity Gel
Кат. номер E6779-1ML E6779-5X1ML E6779-500UL General descriptionEZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody covalently attached to crosslinked agarose beads.SpecificityBinding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.ApplicationEZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis and immunoprecipitation assay.
Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.Features and Benefits- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Other NotesThe affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).Physical form1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm KathonLegal InformationEZview is a trademark of Sigma-Aldrich Co. LLCПараметрыshelf life 1 yr at 28 °C Quality Level 200 analyte chemical class(es) proteins (hemagglutinin) application(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable matrix crosslinked agarose capacity 0.4 mg/mL, gel binding capacity (HA-tagged fusion protein) storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
EZview™ Red Glutathione Affinity Gel
MDL number: MFCD05665380 NACRES: NA.56
Кат. номер E6406-1ML E6406-5X1ML General descriptionEZviewa Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer.ApplicationThe product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Packaging
1 mL in poly bottle
Features and Benefits- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm KathonLegal InformationEZview is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, analyte chemical class(es) proteins (GST) application(s) immunoprecipitation (IP): suitable, protein purification: suitable storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
EZview™ Red HIS-Select® HC Nickel Affinity Gel
MDL number: MFCD03099545
Кат. номер E3528-5X1ML E3528-1ML
Packaging
1 mL in poly bottleApplicationSmall-scale affinity capture of fusion recombinant proteins with His-tags / histidine-containing tags (His-tagged proteins).
When performing small scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Features and Benefits- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm KathonLegal InformationEZview is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, storage temp. 20°C
Safety Informationpictograms GHS07 signalword Warning hcodes H317 pcodes P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 RIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
FLAG® HA Tandem Affinity Purification Kit TP0010-5RXN
NACRES: NA.32 General descriptionTAP technology is a recent development in protein purification. This technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification of protein complexes by adding a tandem affinity tag to a known "bait" protein to pull down endogenous proteins that interact with the targeted protein of interest.ApplicationThe FLAG® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology.
Sutitable for mass spectrometry analysis and western blotting.
Browse additional application references in our FLAG® Literature portal.Legal InformationFLAG is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 storage temp. 2-8°C
Safety Informationpictograms GHS07,GHS09 signalword Warning hcodes H319 - H411 pcodes P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P391 RIDADR NONH for all modes of transport Узнать цену -
GlutathioneAgarose
MDL number: MFCD00131200 NACRES: NA.56
Кат. номер G4510-50ML G4510-1ML G4510-10ML G4510-5ML ApplicationFor use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins from cell lysates of various kinds.
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.Packaging1, 5, 10, 50 mL in poly bottleQuantityOne g powder swells to ≥10 ml of gelPhysical formLyophilized powder stabilized with lactoseПараметрыform lyophilized powder Quality Level 200 analyte chemical class(es) proteins (GST) extent of labeling ≥10 μmol per mL gel application(s) immunoprecipitation (IP): suitable, protein purification: suitable matrix 4% cross-linked beaded agarose matrix activation epoxy matrix attachment sulfur matrix spacer 12 atoms (10 carbon) storage temp. −20°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
HIS-Select® Elution Buffer
NACRES: NA.56
Кат. номер H5413-250ML H5413-250ML-PW H5413-PH H5413-250ML-KC H5413-500ML General descriptionElution buffer is a major solvent in affinity chromatography. Elution buffer is used to wash away unbound proteins at first and at a greater concentration it releases the desired protein from the ligand. It is important that the elution buffer works quickly without changing the function or activity of the desired protein.
Physical form
Contains 250 mM Imidazole, 0.3 M sodium chloride, 50 mM sodium phosphate, pH 8.0.Legal InformationHIS-Select is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200,
Safety Informationpictograms GHS07,GHS08 signalword Danger hcodes H315 - H319 - H360D pcodes P201 - P302 + P352 - P305 + P351 + P338 - P308 + P313 Personal Protective Equipment Faceshields,, Gloves,, Goggles,, type ABEK (EN14387) respirator filter, RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
HIS-Select® Filter Plate
NACRES: NB.24
Кат. номер H0413-5X1EA H0413-1EA General descriptionThe HIS-Select® Filter Plate is an immobilized metal-ion affinity chromatography (IMAC) product that allows rapid purification of small-scale crude cell extracts containing histidine-tagged proteins. The HIS-Select® Filter Plate contains 20 µm, spherical silica particles (100 nm pore size) with a hydrophilic layer.
The HIS-Select® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.ApplicationThe HIS-Select® Filter Plate is suitable for purification of recombinant proteins with histidine tags.Features and Benefits- High Selectivity for his-tagged proteins
- Ready-to-use plate format
- Compatible with CelLytic™ cell lysis reagents
Legal InformationCovered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Sigma-Aldrich Co. LLCПараметрыgrade for molecular biology form plate storage temp. 2-8°C
Safety Informationpictograms GHS08 signalword Danger hcodes H317 - H334 - H341 - H350 - H360D - H372 - H373 - H412 pcodes P202 - P260 - P273 - P280 - P302 + P352 - P308 + P313 Target Organs Respiratory Tract WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
HIS-Select® Spin Columns
NACRES: NA.56
Кат. номер H7787-50EA H7787-10EA General descriptionKit contains 3 collection tubes for every spin column.ApplicationPre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.Features and Benefits- High Selectivity for higher purity
- Convenient and Ready-to-Use
- Consistent protein binding each use
- Compatible with CelLytic™ cell lysis reagents
Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 shelf life 2 yr at 28 °C capacity >0.5 mg/column storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
HIS-Select® Wash Buffer
NACRES: NA.56
Physical formКат. номер H5288-PH H5288-500ML-PW H5288-500ML H5288-1L
Contains 10 mM imidazole, 0.3 M sodium chloride, 50 mM sodium phosphate, pH 8.0.Legal InformationHIS-Select is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200,
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), RIDADR NONH for all modes of transport WGK Germany nwg Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Monoclonal Anti-HAAgarose antibody produced in mouse
MDL number: MFCD00803873 NACRES: NA.56
Кат. номер A2095-1ML A2095-500UL-KC A2095-5X1ML A2095-500UL General descriptionMonoclonal Anti-HA Agarose Conjugate recognizes native as well as denatured-reduced forms of the HA (hemagglutinin)-tagged proteins and is reactive with N-or C-terminal HA-tagged fusion proteins expressed in E.coli or in mammalian cells.SpecificityThe antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.Immunogensynthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLHApplicationSuitable for use in immunoprecipitation assays and for immunoaffinity purification of HA-tagged fusion proteins.Physical formSuspension in 0.01 M phosphate buffered saline, containing 15 mM sodium azidePreparation NotePrepared using cyanogen bromide-activated agarose. Specific antibody concentration is 2.0-2.4 mg/ml settled resin.ПараметрыQuality Level 200 biological source mouse antibody form purified immunoglobulin antibody product type primary antibodies clone HA-7, monoclonal form PBS suspension isotype IgG1 capacity 20-50 nmol/mL elution capacity (HA-tagged fusion protein), 30-50 nmol/mL binding capacity (HA-tagged fusion protein) conjugate agarose conjugate shipped in wet ice storage temp. 2-8°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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