ProteoExtract® Cytosol/Mitochondria Fractionation Kit

Кат. номер

QIA88-1KITCN

QIA88-1KIT

This kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. The enriched fractions can be used to study factors of interest using Western blotting, ELISA, or other assays.
Note: 1 Test = reagents sufficient for processing 5 x 107 cells.
1 kit in Fibre case
Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, and a user protocol.
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
The ProteoExtract Cytosol/Mitochondria Fractionation Kit is designed for extraction of mammalian cells to yield enriched mitochondrial and cytosolic fractions for use in downstream analyses, such as Western blotting.
• Protease Inhibitor Cocktail: Reconstitute the lyophilized Protease Inhibitor Cocktail by adding 250 µl DMSO; mix well before use. Dispense into aliquots and store at -20°C.• 1X Cytosol Extraction Buffer Mix: Prepare 1X Cytosol Extraction Buffer by diluting the Cytosol Extraction Buffer 1:4 with ddH2O. Prepare only the amount needed for the assay (5 x 107 cells require 1 ml 1X Cytosol Extraction Buffer Mix). Just prior to use add 2 µl Protease Inhibitor Cocktail and 1 µl DTT to 1 ml 1X Cytosol Extraction Buffer.• Mitochondria Extraction Buffer Mix: Prepare only the amount needed for the assay (5 x 107 pelleted cells require 100 µl Mitochondria Extraction Buffer Mix). Just prior to use, add 2 µl Protease Inhibitor Cocktail and 1 µl DTT to 1 ml of Mitochondria Extraction Buffer.
Upon arrival store the entire contents of the kit at -20°C. Following initial use, store the buffers at 4°C and the DTT and Protease Inhibitor Cocktail at -20°C.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany

storage conditions	-20C
usage	sufficient for 100 extractions
Торговая марка	Calbiochem®
storage condition	OK to freeze, avoid repeated freeze/thaw cycles
application(s)	DNA extraction: suitable, RNA extraction: suitable, nuclear extraction: suitable, protein extraction: suitable
input	sample type cells
shipped in	dry ice
storage temp.	20°C